Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   AAVE_RS02065 Genome accession   NC_008752
Coordinates   450819..452408 (+) Length   529 a.a.
NCBI ID   WP_011793602.1    Uniprot ID   A1TJ88
Organism   Paracidovorax citrulli AAC00-1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 445819..457408
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAVE_RS02040 (Aave_0414) argS 446167..447876 (+) 1710 WP_011793597.1 arginine--tRNA ligase -
  AAVE_RS02045 (Aave_0415) - 447882..448529 (+) 648 WP_041827195.1 SPOR domain-containing protein -
  AAVE_RS02050 (Aave_0416) - 448599..449255 (+) 657 WP_011793599.1 thiol:disulfide interchange protein DsbA/DsbL -
  AAVE_RS02055 (Aave_0417) lptA 449375..450025 (+) 651 WP_011793600.1 lipopolysaccharide transport periplasmic protein LptA -
  AAVE_RS02060 (Aave_0418) lptB 450028..450819 (+) 792 WP_011793601.1 LPS export ABC transporter ATP-binding protein -
  AAVE_RS02065 (Aave_0419) rpoN/rpoN1 450819..452408 (+) 1590 WP_011793602.1 RNA polymerase factor sigma-54 Machinery gene
  AAVE_RS02070 (Aave_0420) - 452433..452975 (-) 543 WP_011793603.1 TspO/MBR family protein -
  AAVE_RS02075 (Aave_0421) - 453036..454892 (-) 1857 WP_011793604.1 methyl-accepting chemotaxis protein -
  AAVE_RS02080 (Aave_0422) - 455047..456372 (+) 1326 WP_011793605.1 THUMP domain-containing protein -
  AAVE_RS02085 (Aave_0423) - 456362..456844 (+) 483 WP_011793606.1 putative toxin-antitoxin system toxin component, PIN family -

Sequence

Protein


Download         Length: 529 a.a.        Molecular weight: 57887.10 Da        Isoelectric Point: 4.6032

>NTDB_id=27199 AAVE_RS02065 WP_011793602.1 450819..452408(+) (rpoN/rpoN1) [Paracidovorax citrulli AAC00-1]
MKPGLSLRISQHLALTPQLQQSIRLLQLSTLELSQEVEQMLDENPFLERNPDEAPREEFGLLQADAPVSDADRGAEESLY
SPASGAVETAASAPGEGASEGSEAPEAPDAPDWEGDGTVEMAPDDGEWGGEAPARTRNQLGDEDDTDATELAREHESLTD
YLHRQALSLRLSEVDRAALRFLIESLNDDGYLDESLEALAVALAGEEDPEQVEELVHRFTVALRLLQSLEPVGVGARGLS
ECLALQLRALQEDGDAEPEVLQAALRICNQPLEMLARRDVRRLAQGCGAGEECTRAAMALIARLEPRPGRAFAQVERNII
VPDVIVKKSSGRGAAHAFTVQLNPDVMPRLRVHDIYAGALRGNRGGEGHQALQQRLQEARWFIKNIQQRFDTILRVSRAI
VDRQKNFFVHGELAMRPLVLRDIADELGLHESTISRVTTAKYMATPQGTYELKYFFGSGLGTETGGNASSTAVRALIKQF
VASESPAKPLSDSQIAEMLKEQGIECARRTVAKYREALKIAPANLRKAL

Nucleotide


Download         Length: 1590 bp        

>NTDB_id=27199 AAVE_RS02065 WP_011793602.1 450819..452408(+) (rpoN/rpoN1) [Paracidovorax citrulli AAC00-1]
ATGAAGCCGGGACTGTCGCTGCGCATCTCGCAGCATCTGGCGCTGACGCCGCAACTGCAGCAATCGATCCGGCTGCTGCA
GCTGTCCACGCTGGAGCTGTCGCAGGAAGTCGAGCAGATGCTCGACGAGAACCCCTTCCTGGAGCGCAACCCGGACGAGG
CACCGCGCGAGGAGTTCGGGCTGCTGCAGGCGGATGCGCCGGTGAGCGACGCCGACCGCGGGGCGGAGGAGTCCCTCTAT
TCGCCGGCCAGCGGGGCGGTGGAGACGGCGGCCTCGGCGCCGGGCGAGGGCGCATCCGAAGGATCGGAGGCGCCGGAAGC
TCCCGATGCGCCGGACTGGGAGGGCGACGGCACCGTCGAGATGGCGCCCGACGACGGCGAGTGGGGCGGCGAGGCGCCCG
CGCGCACGCGCAACCAGCTGGGGGACGAAGACGACACGGACGCGACCGAACTCGCGCGCGAGCACGAGTCGCTCACCGAT
TACCTGCACCGGCAGGCGCTGTCGCTGCGCCTGTCGGAGGTGGACCGCGCGGCGCTGCGCTTCCTGATCGAATCGCTGAA
CGACGACGGCTACCTCGACGAATCGCTGGAGGCACTGGCCGTGGCCCTCGCGGGCGAGGAAGACCCCGAGCAGGTGGAGG
AACTGGTGCATCGCTTCACGGTGGCGCTGCGGCTGCTGCAGAGCCTGGAGCCCGTGGGCGTGGGCGCGCGCGGCCTGAGC
GAATGCCTGGCGCTGCAGCTGCGGGCCCTGCAGGAAGATGGCGACGCGGAGCCCGAAGTGCTGCAGGCGGCGCTGCGCAT
CTGCAATCAGCCGCTGGAGATGCTGGCGCGGCGCGACGTGCGCCGGCTCGCGCAGGGTTGCGGTGCCGGCGAGGAGTGCA
CGCGCGCGGCGATGGCGCTCATCGCGCGGCTGGAGCCCCGGCCGGGCCGGGCTTTCGCGCAGGTCGAGCGCAACATCATC
GTGCCCGACGTGATCGTGAAGAAGAGCAGTGGCCGCGGGGCCGCGCATGCGTTCACCGTGCAGCTCAACCCGGATGTCAT
GCCGCGGCTGCGCGTGCACGACATCTATGCCGGGGCGCTGCGCGGCAACCGCGGCGGCGAGGGGCACCAGGCGCTGCAGC
AGCGGCTGCAGGAAGCGCGTTGGTTCATCAAGAACATCCAGCAGCGGTTCGACACCATCCTGCGCGTCTCGCGCGCCATC
GTGGACCGGCAGAAGAACTTCTTCGTGCATGGCGAGCTGGCCATGCGGCCGCTCGTGCTGCGCGACATCGCCGACGAACT
GGGGCTGCACGAATCGACGATCAGCCGGGTGACCACGGCCAAGTACATGGCCACGCCCCAGGGTACCTACGAGCTGAAGT
ATTTCTTCGGCTCGGGCCTGGGCACGGAAACGGGCGGCAATGCGTCGAGCACGGCCGTGCGTGCGCTGATCAAGCAGTTC
GTCGCGTCGGAGAGCCCGGCCAAGCCGCTGTCGGACAGCCAGATCGCGGAGATGCTCAAGGAGCAGGGCATCGAATGCGC
GCGCCGCACGGTGGCGAAATACCGCGAGGCGCTGAAGATCGCACCGGCCAACCTGCGCAAGGCGCTGTAA

Domains

Predicted by InterProScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(149-356)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(371-527)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

52.731

100

0.529

Multiple sequence alignment