Detailed information NTG0271663

Bioinformatically predicted

Overview

• Name: comFA
• Type: Machinery gene
• Locus tag: C2I25_RS13275
• Genome accession: NZ_CP026678
• Coordinates: 2550941..2552290 (+)
• Length: 449 a.a.
• NCBI ID: WP_000879410.1
• Uniprot ID: -
• Organism: Bacillus cereus strain TG1-6
• Function: ssDNA transport into the cell (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2545941..2557290

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
C2I25_RS13250 (C2I25_13245)	-	2547051..2547686 (-)	636	WP_106823553.1	YigZ family protein	-
C2I25_RS13255 (C2I25_13250)	-	2547923..2548765 (+)	843	WP_000684727.1	DegV family protein	-
C2I25_RS13265 (C2I25_13260)	-	2548938..2549252 (+)	315	WP_000400857.1	helix-turn-helix domain-containing protein	-
C2I25_RS13270 (C2I25_13265)	-	2549401..2550813 (+)	1413	WP_071731492.1	NlpC/P60 family protein	-
C2I25_RS13275 (C2I25_13270)	comFA	2550941..2552290 (+)	1350	WP_000879410.1	ATP-dependent helicase ComFA	Machinery gene
C2I25_RS13280 (C2I25_13275)	-	2552290..2552994 (+)	705	WP_071731494.1	ComF family protein	-
C2I25_RS13285 (C2I25_13280)	cspC	2553121..2553318 (+)	198	WP_001990088.1	cold shock protein CspC	-
C2I25_RS13290 (C2I25_13285)	hpf	2553640..2554182 (+)	543	WP_000671193.1	ribosome hibernation-promoting factor, HPF/YfiA family	-
C2I25_RS13295 (C2I25_13290)	secA	2554456..2556963 (+)	2508	WP_000579373.1	preprotein translocase subunit SecA	-

Sequence

Protein

• Length: 449 a.a.
• Molecular weight: 51104.99 Da
• Isoelectric Point: 10.0088

>NTDB_id=271663 C2I25_RS13275 WP_000879410.1 2550941..2552290(+) (comFA) [Bacillus cereus strain TG1-6]
MLAGKQLLLDELSSDLQRELNDLKKKGEVVCVQGVKKKASKYVCQRCGNIEQRLFASFLCKRCSKVCTYCRKCITMGRVS
ECALLVRGIAERKREKNLNLLRWSGTLSTGQNLAAQGVIEAIKRKESFFIWAVCGAGKTEMLFYGINEALQKGERVCIAT
PRTDVVLELAPRLQEVFPYIKVAALYGGSVDKEKDAVLVVATTHQLLRYYRAFHVMVVDEIDAFPYCADQMLQYAVKQAM
KERAARIYLTATPDETWKRKFRRGEQKGVIVSGRYHRHPLPVPLFCWCGNWKKSLIHKRIPRVLLQWLQTYLNKKYPIFL
FVPHVRYIEEISLLLKSLNKRIEGVHAEDPGRKEKVAAFRKGEIPLLVTTTILERGVTVKNLQVAVLGAEEEIFSESALV
QIAGRAGRSFEAPYGEVVYFHYGKTEAMVRAKKHIQGMNKNAKEQGLID

Nucleotide

• Length: 1350 bp

>NTDB_id=271663 C2I25_RS13275 WP_000879410.1 2550941..2552290(+) (comFA) [Bacillus cereus strain TG1-6]
ATGCTAGCGGGGAAACAGTTGCTATTAGACGAACTTTCTTCAGATTTACAGAGGGAATTAAATGATTTGAAAAAGAAGGG
AGAGGTCGTCTGTGTACAAGGTGTAAAAAAGAAGGCTTCTAAATATGTATGTCAGCGCTGTGGAAATATAGAACAGCGGC
TATTTGCGTCGTTTTTATGTAAAAGATGCAGTAAAGTGTGCACATATTGCCGGAAGTGTATAACGATGGGGAGGGTAAGT
GAATGTGCTTTACTTGTTCGCGGGATTGCTGAAAGAAAGAGAGAAAAGAATTTAAACTTGTTACGGTGGAGCGGAACGTT
GTCTACTGGTCAGAATTTGGCGGCGCAAGGAGTTATAGAGGCTATTAAGCGAAAAGAATCATTTTTTATTTGGGCTGTAT
GCGGGGCTGGGAAAACAGAGATGTTGTTTTACGGTATTAACGAAGCGCTTCAAAAAGGAGAAAGAGTTTGTATCGCAACG
CCGAGAACGGATGTTGTTCTGGAATTAGCACCGAGATTACAAGAAGTATTTCCATATATAAAGGTAGCGGCTTTATATGG
AGGGAGTGTGGATAAAGAAAAAGATGCAGTACTAGTCGTTGCAACCACGCATCAATTATTGCGTTATTATAGGGCGTTTC
ATGTCATGGTTGTAGATGAGATAGATGCTTTTCCATATTGTGCAGATCAAATGTTACAGTACGCGGTAAAACAAGCGATG
AAAGAAAGAGCGGCGCGTATTTATTTAACTGCGACTCCAGATGAAACGTGGAAGCGAAAATTTAGAAGAGGTGAACAAAA
AGGTGTTATTGTTTCTGGACGATATCACCGTCATCCTTTGCCAGTTCCTTTATTTTGTTGGTGCGGGAATTGGAAAAAAA
GCCTCATTCATAAAAGAATTCCTCGAGTTTTACTACAGTGGTTACAAACGTACTTAAATAAAAAATATCCTATTTTTTTA
TTTGTTCCCCATGTGCGATATATAGAAGAGATAAGCCTGTTGTTAAAGTCATTAAACAAGCGAATTGAAGGTGTACATGC
AGAAGATCCGGGTAGAAAAGAAAAAGTAGCGGCTTTCAGAAAAGGAGAAATCCCATTATTAGTTACAACGACAATTTTAG
AGCGAGGCGTAACAGTAAAAAATTTGCAAGTAGCGGTTTTAGGGGCGGAAGAAGAAATATTCTCAGAAAGTGCACTCGTA
CAAATTGCGGGCCGAGCAGGGCGGAGCTTTGAAGCACCGTATGGAGAGGTCGTTTATTTTCACTATGGTAAGACAGAGGC
GATGGTGCGCGCGAAAAAACATATTCAAGGTATGAATAAAAATGCGAAAGAACAAGGATTGATCGATTAA

Domains

Predicted by InterproScan.

• Type III restriction enzyme, res subunit (ResIII) (108-254)

• Helicase conserved C-terminal domain (Helicase_C) (312-408)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comFA	Bacillus subtilis subsp. subtilis str. 168	50.679	98.441	0.499
comFA	Latilactobacillus sakei subsp. sakei 23K	41.709	88.641	0.37