Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   C2I25_RS11120 Genome accession   NZ_CP026678
Coordinates   2120527..2121903 (-) Length   458 a.a.
NCBI ID   WP_106823379.1    Uniprot ID   -
Organism   Bacillus cereus strain TG1-6     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2115527..2126903
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C2I25_RS11100 (C2I25_11095) ispF 2116896..2117372 (-) 477 WP_000488386.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  C2I25_RS11105 (C2I25_11100) ispD 2117489..2118169 (-) 681 WP_000288307.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  C2I25_RS11110 (C2I25_11105) - 2118186..2119289 (-) 1104 WP_053565451.1 PIN/TRAM domain-containing protein -
  C2I25_RS11115 (C2I25_11110) disA 2119450..2120523 (-) 1074 WP_000392164.1 DNA integrity scanning diadenylate cyclase DisA -
  C2I25_RS11120 (C2I25_11115) radA 2120527..2121903 (-) 1377 WP_106823379.1 DNA repair protein RadA Machinery gene
  C2I25_RS11125 (C2I25_11120) clpC 2121999..2124434 (-) 2436 WP_000971170.1 ATP-dependent protease ATP-binding subunit ClpC Regulator
  C2I25_RS11130 (C2I25_11125) - 2124457..2125521 (-) 1065 WP_000050836.1 protein arginine kinase -
  C2I25_RS11135 (C2I25_11130) - 2125526..2126074 (-) 549 WP_000128385.1 UvrB/UvrC motif-containing protein -
  C2I25_RS11140 (C2I25_11135) ctsR 2126245..2126706 (-) 462 WP_001244560.1 transcriptional regulator CtsR -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 49927.61 Da        Isoelectric Point: 6.9054

>NTDB_id=271653 C2I25_RS11120 WP_106823379.1 2120527..2121903(-) (radA) [Bacillus cereus strain TG1-6]
MAKKKTKFICQECGYQSPKYMGKCPGCGQWNTLVEEMEPVVSSRRLNYANAIQTEVTKPRRLTEVETRSEARIETEFQEF
NRVLGGGIVDGSLVLIGGDPGIGKSTLLLQISSQLADSSYDVLYISGEESAKQIKLRADRLHVKGSNLFVVAETDLQRIA
AHIEEMNPAFVVIDSIQTIHLPEVTSAPGSVAQVRECTAELMKLAKTKGIPIFIVGHVTKEGAIAGPRMLEHMVDAVLYF
EGDRHHTYRILRAVKNRFGSTNEMGIFEMKELGLAEVLNPSEIFLEERPVGVAGSTVVASMEGTRPVLVEIQALISPTSF
GNPRRMATGIDHNRVSLIMAVLEKRTGLLLQNQDAYLKVAGGLKLDEPAIDLAVALSIASSFRDKSTAPTDAVIGEVGLT
GEIRRVSRIEQRVQEAAKLGFQRVIIPRKNLGGWTIPDGIEVVGVSNLGEALRLTLGG

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=271653 C2I25_RS11120 WP_106823379.1 2120527..2121903(-) (radA) [Bacillus cereus strain TG1-6]
ATGGCTAAAAAGAAAACGAAATTTATATGTCAAGAATGTGGTTATCAGTCACCAAAATATATGGGTAAATGTCCTGGATG
TGGTCAATGGAATACACTTGTTGAAGAAATGGAGCCAGTTGTATCATCTAGGCGCCTTAATTACGCGAATGCAATTCAAA
CGGAAGTAACAAAACCAAGACGTCTTACCGAAGTAGAAACAAGGTCTGAGGCACGTATTGAAACGGAATTTCAAGAGTTT
AATCGTGTGCTTGGTGGTGGAATTGTAGATGGGTCCTTAGTGCTTATTGGTGGAGACCCTGGGATTGGAAAATCAACATT
ACTATTACAGATTTCATCACAATTAGCAGATTCTTCATATGATGTATTATACATATCGGGTGAGGAGTCGGCAAAGCAGA
TTAAACTTCGTGCAGATCGTTTGCATGTAAAGGGTAGCAATCTATTTGTTGTAGCAGAGACAGACCTGCAGCGTATTGCA
GCACATATTGAAGAGATGAATCCAGCTTTTGTTGTTATTGATTCTATTCAAACGATACATTTACCTGAAGTGACGTCAGC
ACCAGGAAGTGTGGCACAAGTACGTGAATGTACAGCGGAATTAATGAAACTTGCAAAAACAAAAGGAATCCCTATTTTCA
TTGTCGGGCATGTGACAAAAGAAGGAGCAATTGCAGGACCTCGTATGTTAGAACATATGGTCGATGCAGTTCTTTACTTT
GAAGGAGATCGACACCATACGTATCGTATTTTACGGGCTGTGAAGAACCGTTTTGGTTCCACGAATGAAATGGGTATCTT
TGAAATGAAAGAGCTTGGTCTTGCGGAAGTTTTAAATCCTTCTGAAATCTTCCTTGAGGAAAGGCCGGTTGGTGTTGCAG
GATCAACAGTAGTTGCTTCAATGGAAGGAACAAGACCGGTTTTAGTAGAAATACAAGCATTAATCTCCCCTACTAGTTTT
GGAAATCCTCGAAGAATGGCGACGGGAATTGATCATAACCGTGTTTCGCTTATTATGGCAGTATTAGAAAAAAGAACAGG
TTTACTATTGCAAAATCAAGATGCATATTTAAAAGTAGCTGGTGGTTTGAAATTAGACGAACCGGCAATTGATTTAGCAG
TGGCTTTAAGTATAGCCTCAAGTTTTAGGGATAAATCTACGGCACCAACTGATGCAGTAATAGGAGAAGTAGGATTAACT
GGAGAAATAAGAAGAGTATCAAGAATCGAACAACGTGTACAAGAAGCGGCTAAATTAGGATTTCAACGTGTTATTATTCC
TAGAAAAAACTTAGGAGGATGGACAATTCCGGATGGGATTGAGGTTGTAGGTGTTTCTAATTTAGGAGAAGCGCTTCGTT
TGACATTAGGAGGCTAG

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(61-222)

Subunit ChlI of Mg-chelatase (ChlI)

(363-430)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

74.946

100

0.751
  radA Streptococcus pneumoniae Rx1

64.238

98.908

0.635
  radA Streptococcus pneumoniae D39

64.238

98.908

0.635
  radA Streptococcus pneumoniae R6

64.238

98.908

0.635
  radA Streptococcus pneumoniae TIGR4

64.238

98.908

0.635
  radA Streptococcus mitis SK321

64.238

98.908

0.635
  radA Streptococcus mitis NCTC 12261

64.018

98.908

0.633

Multiple sequence alignment