Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   D0808_RS15330 Genome accession   NZ_CP031783
Coordinates   2996860..2997270 (-) Length   136 a.a.
NCBI ID   WP_009967785.1    Uniprot ID   P42983
Organism   Bacillus subtilis strain MENO2 voucher National Research Centre     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2991860..3002270
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D0808_RS15300 (D0808_15415) - 2991947..2992087 (+) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -
  D0808_RS15305 (D0808_15420) yqeF 2992393..2993124 (-) 732 WP_003229964.1 SGNH/GDSL hydrolase family protein -
  D0808_RS15310 (D0808_15425) cwlH 2993376..2994128 (-) 753 WP_153256220.1 N-acetylmuramoyl-L-alanine amidase CwlH -
  D0808_RS15315 (D0808_15430) yqeD 2994315..2994941 (+) 627 WP_038429330.1 TVP38/TMEM64 family protein -
  D0808_RS15320 (D0808_15435) gnd 2994960..2995853 (-) 894 WP_130572588.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  D0808_RS15325 (D0808_15440) yqeB 2996105..2996827 (+) 723 WP_024572277.1 hypothetical protein -
  D0808_RS15330 (D0808_15445) nucA/comI 2996860..2997270 (-) 411 WP_009967785.1 sporulation-specific Dnase NucB Machinery gene
  D0808_RS15335 (D0808_15450) - 2997466..2997813 (+) 348 Protein_2984 sigma-70 family RNA polymerase sigma factor -
  D0808_RS15340 (D0808_15455) spoIVCA 2997844..2999304 (-) 1461 WP_250635637.1 site-specific DNA recombinase SpoIVCA -
  D0808_RS21095 - 2999262..2999440 (-) 179 Protein_2986 hypothetical protein -
  D0808_RS15345 (D0808_15460) - 2999621..3000316 (-) 696 WP_153256222.1 DUF6022 family protein -
  D0808_RS15350 (D0808_15465) - 3000672..3001619 (-) 948 WP_153256223.1 zinc-binding alcohol dehydrogenase family protein -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14967.97 Da        Isoelectric Point: 5.1853

>NTDB_id=271167 D0808_RS15330 WP_009967785.1 2996860..2997270(-) (nucA/comI) [Bacillus subtilis strain MENO2 voucher National Research Centre]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=271167 D0808_RS15330 WP_009967785.1 2996860..2997270(-) (nucA/comI) [Bacillus subtilis strain MENO2 voucher National Research Centre]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAACAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGAGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGATAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACG
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCGGTCTGCGAGGAAGGCGGCGCAGGGGCTGATGTCCGATATGTGAC
ACCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTATCCTGACGGCACCAGAGTGCTGTTTA
TTGTGCAGTAA

Domains

Predicted by InterProScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(40-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P42983

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

62.609

84.559

0.529