Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   AZO_RS02540 Genome accession   NC_008702
Coordinates   541320..542780 (-) Length   486 a.a.
NCBI ID   WP_011764239.1    Uniprot ID   -
Organism   Azoarcus olearius     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 536320..547780
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AZO_RS02515 (azo0499) - 536846..537652 (-) 807 WP_011764234.1 ABC transporter substrate-binding protein -
  AZO_RS02520 (azo0500) ilvA 537808..539328 (-) 1521 WP_011764235.1 threonine ammonia-lyase, biosynthetic -
  AZO_RS02525 (azo0501) hprK 539453..540394 (-) 942 WP_011764236.1 HPr(Ser) kinase/phosphatase -
  AZO_RS02530 (azo0502) ptsN 540363..540848 (-) 486 WP_011764237.1 PTS IIA-like nitrogen regulatory protein PtsN -
  AZO_RS02535 (azo0503) hpf 540982..541305 (-) 324 WP_011764238.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  AZO_RS02540 (azo0504) rpoN/rpoN1 541320..542780 (-) 1461 WP_011764239.1 RNA polymerase factor sigma-54 Machinery gene
  AZO_RS02545 (azo0505) lptB 542790..543512 (-) 723 WP_011764240.1 LPS export ABC transporter ATP-binding protein -
  AZO_RS02550 (azo0506) recX 543499..543948 (-) 450 WP_011764241.1 recombination regulator RecX -
  AZO_RS02555 (azo0507) recA 543971..545005 (-) 1035 WP_011764242.1 recombinase RecA Machinery gene
  AZO_RS02560 (azo0508) - 545130..545615 (-) 486 WP_011764243.1 CinA family protein -
  AZO_RS02565 (azo0509) - 545618..546100 (-) 483 WP_011764244.1 phosphatidylglycerophosphatase A -
  AZO_RS02570 (azo0510) thiL 546104..547114 (-) 1011 WP_011764245.1 thiamine-phosphate kinase -
  AZO_RS02575 (azo0511) - 547249..547530 (-) 282 WP_011764246.1 RnfH family protein -

Sequence

Protein


Download         Length: 486 a.a.        Molecular weight: 54033.96 Da        Isoelectric Point: 4.8528

>NTDB_id=27082 AZO_RS02540 WP_011764239.1 541320..542780(-) (rpoN/rpoN1) [Azoarcus olearius]
MKPTLQLKLSQHLTLTPQLQQSIKLLQLSTLELNTEIERFLLENPMLEREEMDGGGHDFQAQLSSGTTTASTTTESSGES
GGEAESRADSAESGSFDEGMDWSSGSGSGSSSNRDDDDDVDFQEFQAAGISLRDHLDQQVALSPLSDRDRALVRFLIEAL
DDDGYLHQELEELLELLPPELDVELDDLCIALKHVQSLEPAGIGARTPQECLALQLRAMPAEPVRDLALQIVSQHLELLA
ERNFAKLKKLTGCDDDALRAAHALICRLDPHPGAQHSVEETRYVLPDVVVRKLRGRWTVQLNPEAMPRLRINQLYASILQ
QNRGQGGGLTSQLQEARWLIKNVQQRFDTILRVSQAIVDQQRQFFDHGEVAMRPLTLREIADQLELHESTVSRVTTQKFM
ATPRGVFELKYFFGSHVATDTGGAASSTAIRALIRQLVDAEDRKKPLSDARIAELLGQQGIVVARRTIAKYRESLNIPPV
SLRKTI

Nucleotide


Download         Length: 1461 bp        

>NTDB_id=27082 AZO_RS02540 WP_011764239.1 541320..542780(-) (rpoN/rpoN1) [Azoarcus olearius]
ATGAAGCCCACGCTCCAACTCAAGCTCTCCCAGCACCTGACGCTGACGCCGCAGTTGCAGCAGTCGATCAAGCTGCTGCA
GCTCTCCACGCTCGAACTCAACACCGAGATCGAACGCTTCCTGCTCGAAAACCCGATGCTCGAGCGCGAGGAGATGGACG
GCGGCGGGCACGACTTCCAGGCGCAGCTCTCTTCCGGCACCACCACGGCCTCGACCACGACCGAAAGCAGTGGCGAAAGT
GGCGGCGAAGCGGAGTCGCGCGCCGACAGCGCGGAAAGCGGCAGCTTCGACGAGGGCATGGACTGGTCGTCCGGCTCGGG
CAGCGGCTCGTCGAGCAACCGCGACGACGATGATGACGTCGATTTTCAGGAGTTCCAGGCCGCGGGCATCTCGCTGCGCG
ACCATCTCGACCAGCAGGTCGCGCTGTCTCCGCTGTCCGACCGCGACCGCGCGCTGGTGCGCTTCCTGATCGAGGCGCTC
GACGACGACGGCTACCTGCACCAGGAGCTGGAAGAGTTGCTCGAGCTGCTGCCGCCCGAACTCGATGTCGAACTCGACGA
CCTCTGCATCGCGCTGAAGCATGTGCAAAGCCTCGAGCCGGCCGGCATCGGCGCCCGCACCCCGCAGGAATGCCTCGCGC
TGCAGCTGCGCGCGATGCCGGCCGAGCCGGTACGCGACCTCGCCCTGCAGATCGTCAGCCAGCACCTCGAACTGCTCGCC
GAGCGCAACTTCGCCAAACTCAAGAAACTCACCGGTTGCGACGACGACGCGCTGCGCGCCGCCCACGCGCTGATCTGCCG
GCTCGATCCGCATCCCGGCGCGCAGCATTCGGTCGAGGAAACCCGCTACGTCCTGCCCGACGTCGTGGTGCGCAAGCTGC
GCGGGCGCTGGACCGTGCAACTCAACCCGGAAGCGATGCCGCGCCTGCGCATCAACCAGCTCTACGCCAGCATCCTGCAG
CAGAACCGGGGCCAGGGCGGCGGCCTGACCAGCCAGCTGCAGGAGGCGCGCTGGCTGATCAAGAATGTGCAGCAGCGCTT
CGACACCATCCTGCGTGTCTCCCAGGCCATCGTTGACCAGCAACGGCAGTTCTTCGATCATGGGGAGGTGGCCATGCGGC
CGCTTACACTCCGGGAGATCGCAGACCAGCTCGAGCTGCACGAATCCACCGTATCGCGCGTCACCACCCAGAAGTTCATG
GCGACCCCGCGCGGCGTGTTCGAACTCAAGTATTTCTTCGGCAGCCATGTCGCCACCGATACCGGTGGCGCGGCCTCCTC
CACGGCGATTCGCGCGCTGATTCGCCAGTTGGTGGATGCCGAGGACAGGAAGAAACCCCTGTCCGACGCCCGGATTGCCG
AATTGTTGGGCCAGCAGGGTATCGTGGTCGCCAGGCGGACCATCGCCAAGTATCGCGAATCGCTCAATATCCCGCCGGTC
AGTCTGCGCAAAACGATCTAA

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(330-484)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(122-315)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

57.341

100

0.595

Multiple sequence alignment