Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   RMAG_RS03860 Genome accession   NC_008610
Coordinates   825783..826739 (+) Length   318 a.a.
NCBI ID   WP_011738131.1    Uniprot ID   A1AX49
Organism   Candidatus Ruthia magnifica str. Cm (Calyptogena magnifica)     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 820783..831739
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RMAG_RS03835 (Rmag_0775) - 821283..821576 (+) 294 WP_011738127.1 hypothetical protein -
  RMAG_RS03840 (Rmag_0776) - 822013..822348 (-) 336 WP_011738128.1 gamma-glutamylcyclotransferase family protein -
  RMAG_RS03845 (Rmag_0777) - 822359..823648 (-) 1290 WP_011738129.1 pitrilysin family protein -
  RMAG_RS03850 (Rmag_0778) - 823645..824970 (-) 1326 WP_011738130.1 pitrilysin family protein -
  RMAG_RS03860 (Rmag_0781) pilA 825783..826739 (+) 957 WP_011738131.1 signal recognition particle-docking protein FtsY Machinery gene
  RMAG_RS03865 (Rmag_0782) - 827882..828088 (+) 207 WP_011738132.1 hypothetical protein -
  RMAG_RS03870 (Rmag_0783) hemC 829347..830261 (-) 915 WP_011738133.1 hydroxymethylbilane synthase -

Sequence

Protein


Download         Length: 318 a.a.        Molecular weight: 34797.80 Da        Isoelectric Point: 5.5156

>NTDB_id=27018 RMAG_RS03860 WP_011738131.1 825783..826739(+) (pilA) [Candidatus Ruthia magnifica str. Cm (Calyptogena magnifica)]
MFNFFKKNQPQSENLDVDKSTSLKQLLNKSRQKLGSSLSSLLLGEKEINEDLLETLETLLITADIGINTTDKILESVRKN
ASRKTLKDTESLYQFLKDTLDKLLIEDNQLKTNINETFVILIVGVNGAGKTTLIGKLAKSFQNQGKSIMLAAGDTFRAAA
VEQLKIWGERNQIPVIAQTTGADSASVIFDAYQSAQAKNIDILIADTAGRLHTQDNLMQELAKIKRVIAKQNADAPHETM
LVIDGSSGQNAINQAREFNKAVVLSGISITKLDGTAKGGVLFAIADELNLPIRYIGVGEGIDDLKVFQSKEFINALFD

Nucleotide


Download         Length: 957 bp        

>NTDB_id=27018 RMAG_RS03860 WP_011738131.1 825783..826739(+) (pilA) [Candidatus Ruthia magnifica str. Cm (Calyptogena magnifica)]
ATGTTTAATTTTTTTAAAAAAAATCAACCCCAATCAGAAAATTTAGACGTGGATAAGTCAACTTCATTAAAGCAACTCCT
AAATAAATCTAGACAAAAGTTAGGGTCTAGCTTATCTTCTTTATTATTAGGTGAAAAAGAAATTAATGAAGATTTATTAG
AAACACTAGAAACGCTATTAATCACTGCTGATATTGGCATTAATACTACAGATAAAATACTAGAATCTGTACGTAAAAAT
GCCTCTAGAAAAACCTTAAAAGATACTGAAAGCTTATATCAATTCTTAAAAGATACATTGGACAAATTATTGATCGAAGA
TAATCAACTTAAGACTAATATTAATGAAACTTTTGTTATTCTAATTGTGGGTGTTAATGGTGCTGGTAAAACCACCTTGA
TTGGTAAATTGGCAAAATCTTTTCAAAATCAAGGCAAGTCGATTATGTTAGCAGCAGGCGATACCTTTCGTGCTGCAGCC
GTTGAACAACTTAAAATATGGGGTGAGCGTAATCAAATACCCGTGATTGCACAAACAACGGGTGCCGATTCAGCTTCGGT
TATCTTTGATGCTTATCAATCAGCACAAGCTAAGAATATTGATATTCTAATTGCCGACACTGCAGGACGATTACACACGC
AAGACAACCTTATGCAAGAACTGGCCAAAATTAAGCGCGTCATTGCCAAACAAAATGCAGACGCACCACATGAAACTATG
CTGGTTATTGACGGCAGTTCAGGGCAAAATGCTATCAATCAAGCCCGAGAATTTAACAAAGCAGTTGTTCTAAGTGGTAT
TAGCATAACTAAGCTAGATGGTACTGCCAAAGGTGGGGTATTATTTGCGATTGCCGATGAACTTAATCTACCTATTCGTT
ATATTGGCGTAGGTGAAGGGATTGATGATCTTAAAGTCTTTCAATCCAAGGAATTCATCAACGCTTTATTTGATTAA

Domains

Predicted by InterproScan.

SRP54-type protein, helical bundle domain (SRP54_N)

(34-100)

SRP54-type protein, GTPase domain (SRP54)

(118-317)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A1AX49

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

54.153

94.654

0.513

Multiple sequence alignment