Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   C3F34_RS09320 Genome accession   NZ_CP026412
Coordinates   1975326..1976090 (+) Length   254 a.a.
NCBI ID   WP_171285000.1    Uniprot ID   -
Organism   Acinetobacter sp. ACNIH2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1970326..1981090
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C3F34_RS09290 (C3F34_09290) - 1970636..1971046 (+) 411 WP_016166216.1 hypothetical protein -
  C3F34_RS09295 (C3F34_09295) - 1971199..1972482 (+) 1284 WP_044739036.1 glycosyltransferase family 4 protein -
  C3F34_RS09300 (C3F34_09300) - 1972511..1973062 (+) 552 WP_044739035.1 phosphatase PAP2 family protein -
  C3F34_RS09305 (C3F34_09305) iscX 1973142..1973342 (+) 201 WP_016166213.1 Fe-S cluster assembly protein IscX -
  C3F34_RS09310 (C3F34_09310) ndk 1973469..1973900 (+) 432 WP_016166212.1 nucleoside-diphosphate kinase -
  C3F34_RS09315 (C3F34_09315) rlmN 1974024..1975259 (+) 1236 WP_044739034.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  C3F34_RS09320 (C3F34_09320) pilF 1975326..1976090 (+) 765 WP_171285000.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  C3F34_RS09325 (C3F34_09325) - 1976081..1976875 (+) 795 WP_104441452.1 helix-turn-helix domain-containing protein -
  C3F34_RS09330 (C3F34_09330) ispG 1976896..1978011 (+) 1116 WP_044739032.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  C3F34_RS09335 (C3F34_09335) hisS 1978008..1979303 (+) 1296 WP_104441453.1 histidine--tRNA ligase -
  C3F34_RS09340 (C3F34_09340) - 1979337..1980041 (+) 705 WP_104441454.1 YfgM family protein -

Sequence

Protein


Download         Length: 254 a.a.        Molecular weight: 28608.51 Da        Isoelectric Point: 7.9240

>NTDB_id=269195 C3F34_RS09320 WP_171285000.1 1975326..1976090(+) (pilF) [Acinetobacter sp. ACNIH2]
MSLVGCITTTTSSAPGFKKDPEKAVEVRTQLAAEYIRKGDLDAAKRALDQALEMEPRDSTANMMMGVLLQQEGSPANMEK
AETHFKRAISADPNNAQARNNYGTYLYVVERYNEAIEQLAIAGSTLGYDQRFKALENLGRIYLKLGDTANAEKSFKQALQ
VNPNSYVSMLELSEIFYLRQQIPAATQLYEQFVRLVGQKNQSARALWIGIRIARANGDQMGMQVLVNQLRALFPDSQEYT
RYLQLQYSTEAVWK

Nucleotide


Download         Length: 765 bp        

>NTDB_id=269195 C3F34_RS09320 WP_171285000.1 1975326..1976090(+) (pilF) [Acinetobacter sp. ACNIH2]
ATGTCGTTGGTGGGTTGTATTACCACAACCACCTCATCGGCACCGGGGTTTAAAAAAGACCCCGAGAAAGCAGTAGAAGT
GCGGACTCAATTGGCAGCGGAATATATTCGTAAGGGCGACCTAGATGCGGCAAAACGCGCACTTGATCAAGCATTAGAAA
TGGAACCACGAGATTCCACGGCGAATATGATGATGGGCGTGTTGCTACAGCAAGAAGGTAGCCCTGCCAATATGGAAAAA
GCGGAGACACACTTTAAACGTGCAATTTCTGCCGATCCAAATAATGCGCAAGCTCGTAACAACTATGGTACGTATTTATA
CGTCGTAGAACGTTATAATGAGGCGATTGAACAATTAGCAATTGCTGGTTCGACCTTGGGTTATGATCAGCGATTCAAGG
CATTGGAAAATTTAGGACGTATTTACTTAAAATTGGGTGATACAGCAAATGCTGAAAAATCATTCAAGCAAGCATTGCAA
GTAAACCCGAATTCTTATGTTTCAATGTTAGAGTTGTCGGAAATATTTTATTTAAGACAGCAAATTCCTGCTGCTACACA
GCTATATGAACAATTTGTTCGTCTGGTTGGGCAGAAAAATCAGAGTGCGCGTGCATTATGGATTGGTATTCGTATTGCAC
GAGCCAATGGCGATCAGATGGGGATGCAAGTGTTAGTCAATCAACTGCGCGCATTGTTCCCTGATAGCCAAGAATACACA
CGTTATTTGCAATTACAGTACAGTACTGAGGCCGTATGGAAGTAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_1)

(133-164)

Tetratricopeptide repeat (TPR_17)

(85-117)

Tetratricopeptide repeat (TPR_8)

(26-57)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

77.6

98.425

0.764
  pilF Acinetobacter baylyi ADP1

70.635

99.213

0.701

Multiple sequence alignment