Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   C3F35_RS14525 Genome accession   NZ_CP026387
Coordinates   2987636..2989069 (-) Length   477 a.a.
NCBI ID   WP_032614481.1    Uniprot ID   A0A3E1ZUH7
Organism   Leclercia sp. LSNIH3     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2982636..2994069
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C3F35_RS14495 (C3F35_14500) elbB 2984205..2984864 (+) 660 WP_103792783.1 isoprenoid biosynthesis glyoxalase ElbB -
  C3F35_RS14500 (C3F35_14505) mtgA 2984867..2985586 (+) 720 WP_103792859.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  C3F35_RS14505 (C3F35_14510) npr 2985583..2985855 (-) 273 WP_032614489.1 PTS phosphocarrier protein NPr -
  C3F35_RS14510 (C3F35_14515) rapZ 2985852..2986706 (-) 855 WP_032614487.1 RNase adapter RapZ -
  C3F35_RS14515 (C3F35_14520) ptsN 2986752..2987243 (-) 492 WP_032614485.1 PTS IIA-like nitrogen regulatory protein PtsN -
  C3F35_RS14520 (C3F35_14525) hpf 2987326..2987613 (-) 288 WP_032614483.1 ribosome hibernation promoting factor -
  C3F35_RS14525 (C3F35_14530) rpoN/rpoN1 2987636..2989069 (-) 1434 WP_032614481.1 RNA polymerase factor sigma-54 Machinery gene
  C3F35_RS14530 (C3F35_14535) lptB 2989117..2989842 (-) 726 WP_032614478.1 LPS export ABC transporter ATP-binding protein -
  C3F35_RS14535 (C3F35_14540) lptA 2989849..2990424 (-) 576 WP_032614476.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  C3F35_RS14540 (C3F35_14545) lptC 2990393..2990968 (-) 576 WP_032614475.1 LPS export ABC transporter periplasmic protein LptC -
  C3F35_RS14545 (C3F35_14550) kdsC 2990965..2991531 (-) 567 WP_032614474.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  C3F35_RS14550 (C3F35_14555) kdsD 2991546..2992532 (-) 987 WP_103792784.1 arabinose-5-phosphate isomerase KdsD -
  C3F35_RS14555 (C3F35_14560) - 2992546..2993523 (-) 978 WP_032614470.1 calcium/sodium antiporter -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 54186.01 Da        Isoelectric Point: 4.3155

>NTDB_id=269077 C3F35_RS14525 WP_032614481.1 2987636..2989069(-) (rpoN/rpoN1) [Leclercia sp. LSNIH3]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALDSNPLLEQTDLHDEVETQQTQDNETLDSVDALEQKEMPD
ELPLDASWDEIYTAGTPSGTRADYQDDELPVYQGETTQSLQDYLMWQVELTPFSDTDRAIATSIVDAVDETGYLTVPLDD
ILESIGDDEIDLEEVEAVLKRVQRFDPIGVAARDLRDCLLIQLSQFVKETPWLEEARLIVSEHLDLLANHDFRSLMRVTR
LKEEILKEAVNLIQSLDPRPGQSIQTSEPEYVIPDVLVRKHNGLWTVELNSDSIPRLQINQQYASMCTSSRNDSDNQFIR
SNLQEARWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEFMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTTMLSDQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=269077 C3F35_RS14525 WP_032614481.1 2987636..2989069(-) (rpoN/rpoN1) [Leclercia sp. LSNIH3]
ATGAAGCAAGGTTTGCAATTAAGGCTCAGCCAACAGCTGGCAATGACGCCGCAGTTACAACAGGCCATTCGTCTGTTGCA
GCTGTCGACGCTAGAACTTCAGCAGGAGCTTCAGCAAGCGCTGGACAGTAATCCCCTGCTTGAGCAAACCGATCTTCACG
ACGAGGTAGAAACCCAGCAAACGCAGGATAACGAAACGCTCGACAGCGTCGACGCGCTGGAGCAAAAAGAGATGCCAGAC
GAGCTTCCTCTTGATGCCAGCTGGGATGAGATCTACACCGCCGGCACGCCATCCGGTACGCGCGCTGATTACCAGGATGA
TGAGTTGCCGGTGTATCAGGGCGAAACCACCCAGTCCCTCCAGGATTACCTGATGTGGCAGGTGGAGCTAACCCCGTTTT
CCGATACCGATCGCGCCATTGCCACCTCCATTGTCGATGCCGTCGATGAGACCGGCTATTTAACCGTGCCGCTGGACGAC
ATTCTGGAAAGCATCGGTGATGATGAGATCGATCTTGAAGAGGTCGAAGCGGTCCTGAAGCGCGTGCAGCGTTTTGATCC
GATTGGCGTTGCGGCACGTGATTTACGTGACTGCCTGCTGATTCAGCTTTCGCAGTTCGTCAAAGAGACGCCGTGGCTGG
AAGAGGCTCGTCTGATCGTCAGCGAGCATCTGGATCTGCTGGCCAACCACGACTTCCGCTCGTTGATGCGGGTGACGCGA
TTGAAAGAAGAGATCCTGAAAGAGGCGGTCAATCTTATTCAGTCGTTGGATCCGCGTCCGGGGCAATCCATCCAGACAAG
CGAGCCTGAGTACGTTATTCCCGATGTACTGGTACGCAAGCATAACGGCCTGTGGACCGTGGAGCTGAATTCCGACAGCA
TCCCGCGCCTGCAAATTAATCAGCAATACGCCTCGATGTGCACCAGTTCGCGCAACGACTCGGATAACCAGTTTATCCGC
AGCAACCTGCAGGAGGCGCGCTGGCTGATCAAAAGCCTCGAGAGCCGCAATGATACGCTGCTACGCGTGAGCCGCTGCAT
CGTGGAGCAGCAGCAGGCCTTCTTTGAACAAGGTGAAGAATTTATGAAGCCGATGGTGCTGGCCGATATTGCCCAGGCCG
TCGAAATGCATGAATCAACGATTTCCCGCGTGACGACGCAGAAGTATCTGCACAGCCCGCGCGGTATTTTTGAGCTTAAG
TATTTTTTCTCCAGCCATGTCAATACCGAAGGCGGTGGCGAAGCTTCGTCTACAGCCATCCGGGCGCTGGTGAAAAAATT
GATCGCCGCGGAAAACCCCGCGAAACCACTGAGCGACAGTAAGTTAACCACCATGCTGTCCGATCAAGGTATCATGGTGG
CACGCCGTACTGTTGCGAAGTATCGAGAGTCTTTATCCATTCCGCCGTCCAACCAGCGTAAACAACTGGTCTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3E1ZUH7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.347

100

0.48

Multiple sequence alignment