Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   C2E15_RS02575 Genome accession   NZ_CP026377
Coordinates   563963..565399 (-) Length   478 a.a.
NCBI ID   WP_104955999.1    Uniprot ID   -
Organism   Mixta gaviniae strain DSM 22758     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 558963..570399
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C2E15_RS02550 (C2E15_02550) mtgA 561071..561787 (+) 717 WP_104959051.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  C2E15_RS02555 (C2E15_02555) npr 561792..562064 (-) 273 WP_104955995.1 PTS phosphocarrier protein NPr -
  C2E15_RS02560 (C2E15_02560) rapZ 562061..562915 (-) 855 WP_104955996.1 RNase adapter RapZ -
  C2E15_RS02565 (C2E15_02565) ptsN 562989..563471 (-) 483 WP_174705710.1 PTS IIA-like nitrogen regulatory protein PtsN -
  C2E15_RS02570 (C2E15_02570) hpf 563652..563939 (-) 288 WP_104955998.1 ribosome hibernation promoting factor -
  C2E15_RS02575 (C2E15_02575) rpoN/rpoN1 563963..565399 (-) 1437 WP_104955999.1 RNA polymerase factor sigma-54 Machinery gene
  C2E15_RS02580 (C2E15_02580) lptB 565448..566173 (-) 726 WP_104956000.1 LPS export ABC transporter ATP-binding protein -
  C2E15_RS02585 (C2E15_02585) lptA 566179..566730 (-) 552 WP_104956001.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  C2E15_RS02590 (C2E15_02590) lptC 566702..567277 (-) 576 WP_104956002.1 LPS export ABC transporter periplasmic protein LptC -
  C2E15_RS02595 (C2E15_02595) kdsC 567274..567840 (-) 567 WP_104956003.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  C2E15_RS02600 (C2E15_02600) kdsD 567857..568843 (-) 987 WP_104956004.1 arabinose-5-phosphate isomerase KdsD -
  C2E15_RS02605 (C2E15_02605) - 568858..569838 (-) 981 WP_104956005.1 calcium/sodium antiporter -

Sequence

Protein


Download         Length: 478 a.a.        Molecular weight: 53961.79 Da        Isoelectric Point: 4.3602

>NTDB_id=268973 C2E15_RS02575 WP_104955999.1 563963..565399(-) (rpoN/rpoN1) [Mixta gaviniae strain DSM 22758]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQEIQLALESNPLLEQTDNVHEEVDSREYVESEALDTREALEQKDMP
EELPLDATWDEIYTAGTPSGTGTDYRDDELPVYQGETTQTLQDYLMWQVELTPFTDTDRAIATSIVDAIDDTGYLTVSLD
EIRDSMGDDEVALDEVEAVLKRIQRFDPIGVGARDLRDCLLVQLSQFASDTPWLTEAQLIVSEHLDLLANHDFRSLMRVT
RLKEDVLKEAMALIQSLDPRPGQSVNTSEPEYVIPDVLVRKIGTRWTVELNGDSVPRLNINQQYAALGSSARNDSDSQFI
RTNLQEAKWLIKSLESRNDTLLKVTRCIVEQQQAFFEQGEEFMRPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFEL
KYFFSSHVNTEGGGEASSTAIRALVKKLISAENPAKPLSDSKLTSMLSEQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1437 bp        

>NTDB_id=268973 C2E15_RS02575 WP_104955999.1 563963..565399(-) (rpoN/rpoN1) [Mixta gaviniae strain DSM 22758]
ATGAAGCAAGGTTTGCAACTCAGGCTCAGCCAACAGCTCGCCATGACGCCGCAGTTGCAGCAGGCTATTCGCCTGCTGCA
GCTTTCCACGCTGGAGCTTCAACAGGAGATTCAGCTGGCGCTGGAGAGCAATCCGTTGCTTGAGCAGACCGACAATGTCC
ATGAAGAGGTGGATTCCCGCGAATATGTCGAGAGCGAAGCGCTCGATACGCGGGAGGCGCTGGAGCAAAAGGATATGCCG
GAAGAGTTGCCGCTGGACGCCACCTGGGACGAAATCTACACTGCCGGCACCCCGTCGGGCACCGGGACGGATTATCGCGA
TGACGAGCTGCCGGTTTATCAGGGCGAAACCACCCAAACGCTACAGGATTACCTGATGTGGCAGGTGGAGCTGACCCCTT
TTACCGATACCGATCGCGCCATCGCCACCTCGATTGTCGACGCCATTGATGATACCGGTTACCTCACCGTTTCGCTGGAC
GAGATCCGCGACAGCATGGGCGATGACGAGGTGGCGCTGGACGAAGTCGAAGCGGTGCTGAAGCGCATTCAGCGTTTCGA
TCCTATCGGCGTCGGCGCCCGCGATCTGCGTGACTGCCTGTTGGTACAGCTTTCACAGTTCGCCAGCGATACGCCATGGC
TGACGGAGGCGCAGCTGATCGTCAGCGAGCATCTCGATCTGCTGGCGAACCACGATTTCCGCAGCCTGATGCGCGTTACC
CGCCTGAAAGAGGATGTGCTGAAAGAGGCGATGGCGCTGATTCAGTCACTCGATCCACGGCCGGGGCAGTCGGTAAATAC
CAGCGAACCGGAATATGTGATCCCCGATGTGCTGGTGCGCAAAATCGGCACGCGCTGGACAGTGGAGCTTAACGGCGACA
GCGTGCCGCGTCTGAACATCAACCAGCAGTATGCGGCACTGGGCAGTTCGGCGCGCAATGACAGTGACAGCCAGTTTATT
CGCACCAATCTGCAGGAAGCGAAGTGGCTGATCAAAAGCCTGGAAAGCCGCAACGATACGCTGCTGAAAGTGACGCGCTG
CATCGTCGAGCAGCAGCAGGCATTCTTTGAACAGGGCGAAGAGTTTATGCGTCCGATGGTGCTGGCGGATATCGCGCAGG
CCGTTGAGATGCATGAATCGACCATTTCCCGCGTGACCACCCAAAAATACCTGCACAGCCCGCGCGGTATTTTCGAACTG
AAATATTTTTTCTCCAGCCACGTCAATACCGAGGGCGGCGGCGAAGCCTCCTCCACGGCGATCCGTGCGCTGGTGAAAAA
ACTCATCTCGGCGGAAAACCCCGCCAAACCGCTGAGCGACAGTAAGCTCACCTCCATGCTGTCCGAACAGGGCATTATGG
TGGCGCGCCGCACCGTCGCGAAGTACCGAGAGTCTTTATCCATCCCGCCATCAAATCAGCGTAAACAGCTGGTTTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(318-476)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(117-304)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.635

100

0.481

Multiple sequence alignment