Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   C1N50_RS01715 Genome accession   NZ_CP026321
Coordinates   344346..345362 (+) Length   338 a.a.
NCBI ID   WP_103412635.1    Uniprot ID   -
Organism   Vibrio campbellii strain BoB-53     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 339346..350362
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C1N50_RS01700 (C1N50_01700) - 339711..340439 (-) 729 WP_005432732.1 redoxin family protein -
  C1N50_RS01705 (C1N50_01705) oxyR 340614..341522 (+) 909 WP_005432705.1 DNA-binding transcriptional regulator OxyR -
  C1N50_RS01710 (C1N50_01710) - 341600..344167 (-) 2568 WP_038889585.1 penicillin-binding protein 1A -
  C1N50_RS01715 (C1N50_01715) pilM 344346..345362 (+) 1017 WP_103412635.1 type IV pilus biogenesis protein PilM Machinery gene
  C1N50_RS01720 (C1N50_01720) pilN 345346..345921 (+) 576 WP_038889584.1 PilN domain-containing protein Machinery gene
  C1N50_RS01725 (C1N50_01725) pilO 345914..346507 (+) 594 WP_005432718.1 type 4a pilus biogenesis protein PilO Machinery gene
  C1N50_RS01730 (C1N50_01730) pilP 346497..347012 (+) 516 WP_005432702.1 pilus assembly protein PilP Machinery gene
  C1N50_RS01735 (C1N50_01735) pilQ 347047..348789 (+) 1743 WP_050906101.1 type IV pilus secretin PilQ Machinery gene
  C1N50_RS01740 (C1N50_01740) aroK 348978..349496 (+) 519 WP_010443904.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37846.27 Da        Isoelectric Point: 6.3141

>NTDB_id=268111 C1N50_RS01715 WP_103412635.1 344346..345362(+) (pilM) [Vibrio campbellii strain BoB-53]
MDKLLVTGIDIGHNSLKAVVLKPNGNSYALLGYKEIILKEGIVAENNTINHQEIVKTLKEMKKELPFRARKVAISVPDNA
VISKKLQIEQNLEESEKEFAVVQAFSHQSPFPVEELSLDFIKVDTDEQMRGTDSYQVFATRKDVVETRVEALQKSGLKPV
LVDVHSQSLGHMWKLVAERFPEKNKYCLLDIGSLNSSFTMFTEQGELFHKEFPCGTRSTGSLFQQDPLADDAQVATEQFN
RQIVERVKRQMQLYTSINGSQDIKGVWLSGEGAATPMLAEELSSQLALECELLNPLGLFEMKVSKRKRRAADWQHFTAAA
GLAVRGIQWLGGDNAVSH

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=268111 C1N50_RS01715 WP_103412635.1 344346..345362(+) (pilM) [Vibrio campbellii strain BoB-53]
ATGGATAAGCTATTAGTTACGGGCATAGATATTGGCCATAACAGCCTCAAAGCCGTGGTACTTAAACCCAATGGCAACAG
TTACGCACTACTGGGTTACAAAGAAATAATACTTAAGGAAGGTATTGTCGCTGAAAATAACACCATAAATCATCAAGAAA
TTGTAAAGACCCTTAAAGAGATGAAAAAAGAGCTACCTTTTCGTGCTCGAAAGGTCGCAATTTCAGTTCCAGATAATGCA
GTGATCAGTAAAAAGCTGCAGATAGAGCAGAACTTAGAAGAGAGTGAAAAGGAGTTTGCTGTTGTACAAGCCTTCTCACA
TCAATCTCCATTCCCCGTGGAAGAGCTGAGTCTTGACTTCATCAAGGTTGATACGGATGAGCAGATGCGAGGCACTGACA
GTTATCAGGTCTTCGCGACTCGTAAAGACGTGGTAGAAACCCGAGTTGAGGCATTGCAGAAATCAGGCTTGAAACCAGTA
CTGGTGGACGTGCACTCACAAAGTCTTGGTCATATGTGGAAATTGGTTGCGGAACGTTTTCCTGAAAAGAACAAGTATTG
TTTACTGGACATTGGTTCTTTGAACAGTTCTTTCACTATGTTCACCGAACAAGGCGAATTGTTTCATAAAGAATTTCCCT
GCGGTACTCGCTCTACGGGCAGTTTGTTTCAGCAAGATCCGCTAGCAGACGATGCACAAGTGGCAACGGAGCAATTTAAT
CGACAAATCGTTGAGCGTGTTAAACGTCAGATGCAGCTCTATACCTCAATTAATGGAAGCCAGGATATCAAAGGCGTTTG
GCTTTCGGGAGAGGGCGCTGCAACACCTATGTTGGCCGAGGAGCTGTCTAGCCAGTTGGCACTAGAGTGTGAACTGCTTA
ATCCACTTGGTTTGTTTGAAATGAAGGTGTCTAAACGCAAACGTCGTGCCGCGGATTGGCAACACTTCACTGCTGCAGCC
GGACTGGCTGTACGTGGTATTCAGTGGCTAGGAGGTGACAATGCTGTATCACATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio campbellii strain DS40M4

97.929

100

0.979
  pilM Vibrio cholerae strain A1552

49.415

100

0.5

Multiple sequence alignment