Detailed information    

insolico Bioinformatically predicted

Overview

Name   pepF   Type   Regulator
Locus tag   C0971_RS04815 Genome accession   NZ_CP026143
Coordinates   949129..950946 (+) Length   605 a.a.
NCBI ID   WP_004433617.1    Uniprot ID   I3E7R1
Organism   Bacillus methanolicus strain DFS2     
Function   degradation of XIP; competence shut-off (predicted from homology)   
Competence regulation

Genomic Context

Location: 944129..955946
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C0971_RS04795 (C0971_04850) spxA 944719..945114 (+) 396 WP_004433609.1 transcriptional regulator SpxA -
  C0971_RS04800 (C0971_04855) mecA 945442..946110 (+) 669 WP_004433611.1 adaptor protein MecA Regulator
  C0971_RS04805 (C0971_04860) cls 946195..947706 (+) 1512 WP_004433613.1 cardiolipin synthase -
  C0971_RS04810 (C0971_04865) - 947806..949002 (+) 1197 WP_248893147.1 competence protein CoiA family protein -
  C0971_RS04815 (C0971_04870) pepF 949129..950946 (+) 1818 WP_004433617.1 oligoendopeptidase F Regulator
  C0971_RS04820 (C0971_04875) - 951536..952420 (-) 885 WP_004433620.1 ClpXP adapter SpxH family protein -
  C0971_RS04825 (C0971_04880) - 952417..952824 (-) 408 WP_248893149.1 globin -
  C0971_RS04830 (C0971_04885) - 953057..953686 (-) 630 WP_248893151.1 lytic transglycosylase domain-containing protein -
  C0971_RS04835 (C0971_04890) - 953709..954287 (-) 579 WP_248893153.1 CYTH domain-containing protein -
  C0971_RS04840 (C0971_04895) - 954424..954783 (+) 360 WP_004433628.1 hypothetical protein -
  C0971_RS04845 (C0971_04900) - 954852..955487 (+) 636 WP_004433630.1 GTP pyrophosphokinase family protein -

Sequence

Protein


Download         Length: 605 a.a.        Molecular weight: 69989.95 Da        Isoelectric Point: 5.1656

>NTDB_id=267510 C0971_RS04815 WP_004433617.1 949129..950946(+) (pepF) [Bacillus methanolicus strain DFS2]
MSNNKAVKKLPARNEIPVEETWRLEDIFATDDEWEKEFEEVKKLIPGINRYKGKLGENADELYNALTFQDQLLERLGKLY
TYAHMRYDQDTTNSFYQGMDDRIKNLYSQAASALAFIVPEILSIEEQKVAAFLEEKEELRLYKHALEEINLQRPHVLSAE
QEALLAEAAEVMNASSNTFGMLNNADLVFPSIKDENGEEVEVTHGRYIRFLESSDRRVRKDAFMAVYSTYGKYRNTFAST
LSGTVKKDNFNARVRKYNSARHAALAANNIPESVYDNLVNTINDNLHLLHRYVKLRKKVLGLDKLHMYDLYTPLVKDVKM
EITYEEAKEIIVKGLAPLGEDYVNVLKEGFENRWVDVRENKGKRSGAYSSGAYGTNPYILMNWQDNVNNLFTLAHEFGHS
VHSYFTRKNQPYPYGNYSIFVAEVASTCNEALLNDYLLKTIDDEKKRLYLLNHYLEGFRGTVFRQTMFAEFEHLIHQKAQ
NHEPLTADTLTKEYYELNKKYFGLEDIEIDEEIGLEWARIPHFYYNYYVYQYATGFSAATALSKQILEEGEPAVSRYIEF
LKAGSSDYPIEVLKKAGVDMTSPKPIEEACKVFEEKLNEMESLLS

Nucleotide


Download         Length: 1818 bp        

>NTDB_id=267510 C0971_RS04815 WP_004433617.1 949129..950946(+) (pepF) [Bacillus methanolicus strain DFS2]
ATGTCAAATAATAAAGCAGTGAAAAAATTGCCAGCTAGAAATGAAATTCCAGTTGAAGAAACTTGGAGGCTAGAGGATAT
TTTTGCAACTGATGATGAATGGGAAAAAGAATTTGAAGAAGTAAAGAAGTTAATTCCTGGTATTAATCGTTATAAAGGGA
AGCTCGGAGAAAATGCAGATGAGTTATATAATGCGCTAACATTTCAGGACCAACTATTAGAACGCCTTGGAAAACTGTAT
ACATACGCGCATATGCGTTATGACCAAGATACAACCAATTCTTTTTACCAAGGAATGGATGACAGAATAAAAAACCTTTA
TTCACAAGCAGCGAGCGCTCTTGCTTTTATTGTTCCTGAAATATTGTCAATTGAAGAACAAAAGGTTGCCGCCTTCTTAG
AAGAAAAAGAAGAATTGAGGTTGTATAAACACGCATTAGAAGAAATCAATTTACAAAGGCCTCATGTCCTTTCTGCCGAG
CAGGAGGCACTTTTGGCAGAAGCAGCTGAGGTCATGAATGCATCAAGCAATACATTCGGAATGTTAAATAATGCAGATCT
TGTGTTTCCGAGCATTAAAGATGAGAACGGAGAAGAAGTAGAAGTAACTCATGGCCGGTACATCCGATTCCTTGAAAGCA
GCGACCGCAGGGTTCGAAAAGATGCATTTATGGCAGTTTACAGCACTTATGGGAAGTACCGGAATACATTTGCCAGCACT
TTAAGCGGTACGGTGAAAAAGGATAATTTTAACGCACGAGTTCGTAAATACAACTCGGCCCGCCATGCTGCATTGGCTGC
AAATAACATTCCAGAAAGTGTATACGATAATCTTGTAAATACAATAAATGATAATCTTCATTTATTGCACCGTTACGTCA
AGCTGCGAAAAAAAGTTCTTGGTTTAGATAAACTTCATATGTATGATTTATACACTCCACTTGTTAAAGACGTGAAAATG
GAAATTACTTATGAAGAGGCAAAGGAGATCATTGTAAAGGGCCTGGCCCCGTTGGGAGAAGATTACGTAAATGTGCTAAA
AGAAGGTTTTGAGAACCGCTGGGTTGACGTACGTGAAAATAAGGGCAAGCGAAGCGGGGCCTACTCTTCAGGAGCATATG
GAACAAATCCATATATCTTAATGAATTGGCAGGACAATGTAAATAATTTATTTACACTAGCCCACGAATTTGGCCATTCT
GTTCACAGTTACTTTACACGCAAAAATCAGCCTTACCCTTATGGCAACTATTCAATATTTGTCGCTGAGGTTGCTTCAAC
ATGTAATGAAGCACTGTTGAATGATTATCTTTTAAAAACAATTGATGATGAGAAAAAACGTCTTTATTTGCTGAATCATT
ATTTGGAAGGGTTCAGAGGAACGGTGTTTCGCCAAACGATGTTTGCAGAATTTGAACATCTTATTCATCAAAAAGCACAA
AATCATGAGCCATTGACAGCTGATACGTTAACGAAAGAATATTATGAACTAAATAAGAAATATTTTGGTTTGGAAGATAT
TGAAATTGATGAAGAAATTGGACTCGAGTGGGCCAGAATACCTCATTTTTATTACAATTATTATGTGTACCAATATGCAA
CCGGCTTCAGTGCTGCAACAGCATTAAGCAAACAAATTCTTGAAGAAGGCGAGCCGGCGGTAAGCCGTTATATTGAATTT
TTAAAAGCTGGCAGTTCCGATTATCCGATTGAGGTGCTCAAAAAAGCAGGAGTTGACATGACGAGTCCAAAACCGATTGA
AGAAGCATGTAAAGTTTTTGAAGAAAAATTAAATGAAATGGAAAGTTTGCTTTCATAA

Domains

Predicted by InterproScan.

Peptidase family M3 (Peptidase_M3)

(211-591)

Oligopeptidase F (Peptidase_M3_N)

(120-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB I3E7R1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pepF Streptococcus salivarius strain HSISS4

51.52

97.851

0.504

Multiple sequence alignment