Detailed information    

insolico Bioinformatically predicted

Overview

Name   recF   Type   Machinery gene
Locus tag   C0971_RS00020 Genome accession   NZ_CP026143
Coordinates   3086..4204 (+) Length   372 a.a.
NCBI ID   WP_003347100.1    Uniprot ID   I3EC06
Organism   Bacillus methanolicus strain DFS2     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1..9204
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C0971_RS00005 (C0971_00005) dnaA 1..1347 (+) 1347 WP_003347093.1 chromosomal replication initiator protein DnaA -
  C0971_RS00010 (C0971_00010) dnaN 1524..2660 (+) 1137 WP_003347096.1 DNA polymerase III subunit beta -
  C0971_RS00015 (C0971_00015) yaaA 2852..3067 (+) 216 WP_003347098.1 S4 domain-containing protein YaaA -
  C0971_RS00020 (C0971_00020) recF 3086..4204 (+) 1119 WP_003347100.1 DNA replication/repair protein RecF Machinery gene
  C0971_RS00025 (C0971_00025) gyrB 4571..6478 (+) 1908 WP_248894040.1 DNA topoisomerase (ATP-hydrolyzing) subunit B -

Sequence

Protein


Download         Length: 372 a.a.        Molecular weight: 43095.63 Da        Isoelectric Point: 9.4963

>NTDB_id=267495 C0971_RS00020 WP_003347100.1 3086..4204(+) (recF) [Bacillus methanolicus strain DFS2]
MFIEELSLKNYRNYEELEAQFENKVNVILGENAQGKTNVMESIYVLAMAKSHRTSKDKDLIRWDQDYAKIEGRVKKNHGS
LPIHLVISKKGKKAKVNHIEQQKLSQYIGNMNVVMFAPEDLHLVKGNPQVRRRFIDMEIGQVSAVYLHEMSQYQKILQQR
NHYLKLLQTRKQNDQAILEILTEQFIGIAVRIIKKRFEFLQLLQNWAMPIHKGISRGLETLKIEYKPSVEVSEDQDLSKM
IKVFEDKFLKVKNREIERGVSQFGPHRDDLLFFVNGRDVQTFGSQGQQRTTALSVKLAEIELIHSEIGEYPILLLDDVLS
ELDNYRQSHLLNTIQGKVQTFVTTTSVDGIDHQTLKEASTYKVEAGVMKRVQ

Nucleotide


Download         Length: 1119 bp        

>NTDB_id=267495 C0971_RS00020 WP_003347100.1 3086..4204(+) (recF) [Bacillus methanolicus strain DFS2]
ATGTTTATTGAGGAGCTGTCTTTGAAAAATTATCGGAATTATGAGGAACTCGAAGCCCAATTTGAAAATAAAGTAAATGT
TATTCTTGGTGAAAATGCCCAAGGGAAAACAAACGTTATGGAATCTATTTATGTATTGGCAATGGCCAAATCGCATCGCA
CCTCAAAAGATAAAGATCTTATTCGCTGGGATCAGGATTATGCTAAAATAGAAGGTAGGGTAAAGAAAAATCATGGTTCC
CTGCCTATTCATTTGGTGATTTCCAAAAAAGGGAAAAAAGCAAAAGTAAACCATATCGAACAGCAAAAACTCAGCCAATA
TATTGGAAATATGAATGTTGTAATGTTTGCGCCTGAAGATCTCCATCTTGTAAAAGGGAACCCTCAAGTAAGGCGTCGTT
TTATTGATATGGAAATTGGTCAAGTTTCAGCTGTTTACTTACATGAAATGAGCCAATATCAAAAAATATTACAACAGCGC
AATCATTATTTAAAACTTTTGCAAACCCGAAAGCAAAACGATCAGGCGATTCTTGAAATACTGACGGAGCAATTTATTGG
AATAGCTGTTCGGATTATCAAAAAGCGTTTCGAGTTTTTACAGCTTCTTCAAAACTGGGCAATGCCGATCCATAAAGGGA
TTTCAAGAGGGCTGGAAACGTTAAAAATTGAGTATAAACCATCGGTTGAAGTATCAGAAGACCAAGATTTGTCGAAAATG
ATAAAAGTATTTGAAGATAAATTTTTAAAAGTCAAAAATCGGGAAATAGAACGCGGAGTTTCACAGTTTGGTCCCCATCG
GGATGACCTTCTTTTTTTTGTAAATGGCAGGGATGTACAAACATTTGGTTCGCAAGGACAGCAGCGGACAACAGCCCTGT
CTGTGAAATTGGCAGAAATAGAGCTTATTCATTCGGAGATTGGCGAATATCCAATCTTGCTGTTGGATGATGTTCTCTCT
GAGTTGGATAATTACCGCCAATCTCATTTGTTAAATACCATTCAAGGCAAGGTTCAGACGTTTGTTACAACGACAAGTGT
TGATGGAATTGACCATCAAACTTTAAAAGAAGCTTCCACCTACAAAGTAGAAGCCGGGGTCATGAAACGGGTTCAATGA

Domains

Predicted by InterproScan.

RecF/RecN/SMC N terminal domain (SMC_N)

(2-346)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB I3EC06

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recF Bacillus subtilis subsp. subtilis str. 168

69.293

98.925

0.685

Multiple sequence alignment