Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   BCEN2424_RS13950 Genome accession   NC_008542
Coordinates   3087596..3089092 (+) Length   498 a.a.
NCBI ID   WP_011546239.1    Uniprot ID   -
Organism   Burkholderia cenocepacia HI2424     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3082596..3094092
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BCEN2424_RS13925 (Bcen2424_2786) kdsD 3083827..3084810 (+) 984 WP_011546235.1 arabinose 5-phosphate isomerase KdsD -
  BCEN2424_RS13930 (Bcen2424_2787) - 3084810..3085346 (+) 537 WP_011546236.1 HAD family hydrolase -
  BCEN2424_RS13935 (Bcen2424_2788) lptC 3085350..3085964 (+) 615 WP_006477790.1 LPS export ABC transporter periplasmic protein LptC -
  BCEN2424_RS13940 (Bcen2424_2789) lptA 3085985..3086650 (+) 666 WP_011546237.1 lipopolysaccharide transport periplasmic protein LptA -
  BCEN2424_RS13945 (Bcen2424_2790) lptB 3086647..3087423 (+) 777 WP_006477788.1 LPS export ABC transporter ATP-binding protein -
  BCEN2424_RS13950 (Bcen2424_2791) rpoN/rpoN1 3087596..3089092 (+) 1497 WP_011546239.1 RNA polymerase factor sigma-54 Machinery gene
  BCEN2424_RS13955 (Bcen2424_2792) raiA 3089251..3089610 (+) 360 WP_006477786.1 ribosome-associated translation inhibitor RaiA -
  BCEN2424_RS13960 (Bcen2424_2793) - 3089805..3090317 (+) 513 WP_027806888.1 PTS sugar transporter subunit IIA -
  BCEN2424_RS13965 (Bcen2424_2794) hprK 3090447..3091415 (+) 969 WP_006483133.1 HPr(Ser) kinase/phosphatase -
  BCEN2424_RS13970 (Bcen2424_2795) rapZ 3091490..3092398 (+) 909 WP_006477783.1 RNase adapter RapZ -
  BCEN2424_RS13975 (Bcen2424_2796) - 3092426..3093061 (+) 636 WP_011546241.1 LON peptidase substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 498 a.a.        Molecular weight: 54397.44 Da        Isoelectric Point: 4.8683

>NTDB_id=26735 BCEN2424_RS13950 WP_011546239.1 3087596..3089092(+) (rpoN/rpoN1) [Burkholderia cenocepacia HI2424]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVAMAVAQNPLLENDDEWIASPLRVAADGSLIAQTPPAQNTEPTA
TNGSSTSSERDEPAGADEYDGYGSGDGNDGPQWNLDEYGRSSGASDDDDLPPLQVQEAATSLRDHLSAQLRVTQAGPRDR
ALVMFLIESLDDDGYLTATLDEVLADLPPELEIDGDELNAALALLHSFDPAGVGARSASECLKLQLLRLDPSPTRTLALE
IVSQHLELLAARDFTRLRKHLKASDDALRDAHALIRSLEPFPGAAYGKAEADYVVPDIIVKKAGQGWLAELNPEVVPRLR
INNLYANILRNSRGDPSAGSLKQQLQEARWLIKNIQQRFDTILRVAQAIVERQKNFFAHGEIAMRPLVLREIADTLGLHE
STVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDPKSPLSDSRIAELLAEQGFVVARRTV
AKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1497 bp        

>NTDB_id=26735 BCEN2424_RS13950 WP_011546239.1 3087596..3089092(+) (rpoN/rpoN1) [Burkholderia cenocepacia HI2424]
ATGAAAGCCAGCCTTCAACTCCGCCTGTCGCAACATCTTGCGCTGACGCCTCAGCTGCAGCAGTCGATCCGGCTCCTGCA
GTTGTCGACGCTCGAATTGCAGCAGGAAGTCGCGATGGCCGTCGCGCAGAACCCGCTGCTCGAGAACGACGACGAATGGA
TCGCCAGCCCGCTGCGCGTCGCGGCGGACGGATCGCTGATCGCGCAGACGCCGCCCGCACAGAACACCGAGCCCACCGCG
ACCAACGGCAGCAGCACGTCCAGCGAGCGCGACGAGCCGGCCGGCGCCGACGAATACGACGGCTACGGGTCCGGTGACGG
CAACGACGGCCCGCAGTGGAATCTCGACGAATACGGCCGCTCGAGCGGCGCGTCCGACGACGACGACCTGCCGCCGCTGC
AGGTGCAGGAAGCCGCGACGTCGCTGCGCGATCATCTCTCGGCGCAGTTGCGCGTCACCCAGGCCGGCCCGCGCGATCGC
GCGCTCGTGATGTTCCTGATCGAATCGCTCGACGACGATGGCTACCTGACCGCCACGCTCGACGAAGTACTCGCCGACCT
GCCGCCCGAACTGGAAATCGACGGCGACGAACTGAATGCGGCGCTCGCGCTGCTGCACAGCTTCGACCCGGCCGGCGTCG
GCGCGCGCTCGGCCTCCGAATGCCTGAAGCTGCAGCTGCTGCGGCTCGATCCGTCCCCCACGCGCACGCTCGCGCTCGAG
ATCGTGTCGCAGCATCTCGAACTGCTCGCCGCACGCGACTTCACACGGTTGCGCAAGCACCTGAAGGCGAGCGACGACGC
GCTGCGCGACGCGCACGCGCTGATCCGCTCGCTGGAGCCGTTCCCCGGCGCCGCCTACGGCAAGGCCGAGGCCGACTACG
TCGTGCCCGACATCATCGTGAAGAAGGCCGGCCAGGGATGGCTCGCGGAGCTCAATCCGGAGGTCGTGCCGCGCCTGCGG
ATCAACAACCTGTACGCGAACATCCTGCGCAACAGCCGCGGCGATCCGTCGGCCGGATCGTTGAAGCAGCAGCTGCAGGA
AGCTCGCTGGTTGATCAAGAACATCCAGCAGCGTTTCGACACGATCCTGCGTGTCGCGCAGGCTATTGTCGAGCGTCAGA
AGAATTTCTTTGCGCACGGTGAAATTGCCATGCGCCCCTTGGTTTTGCGGGAAATAGCTGATACGCTGGGCCTACACGAG
TCGACTGTCAGCCGTGTGACAACCGGGAAGTACATGCTGACCCCGTTCGGGACGCTTGAATTTAAGTACTTCTTCGGATC
TCACGTCTCGACCGACACCGGTGGCGCCGCCTCGTCGACCGCCATCCGAGCCCTCATCAAGCAACTGATAGGAGCTGAAG
ACCCAAAATCGCCACTTTCGGACAGCCGCATTGCCGAGCTGCTGGCAGAACAGGGTTTCGTGGTCGCGCGCCGCACGGTT
GCGAAATATCGCGAAGCCCTCAAGATCCCGGCAGTGAATCTGCGCAAGTCTCTTTAA

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(341-496)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(134-325)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

67.921

100

0.689

Multiple sequence alignment