Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   HLI_RS10845 Genome accession   NZ_CP026118
Coordinates   2150741..2152120 (-) Length   459 a.a.
NCBI ID   WP_128524988.1    Uniprot ID   A0A410MD79
Organism   Halobacillus litoralis strain ERB 031     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2145741..2157120
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HLI_RS10830 (HLI_11015) - 2147114..2148310 (-) 1197 WP_347232244.1 bifunctional 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase/2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  HLI_RS10835 (HLI_11020) - 2148412..2149512 (-) 1101 WP_128524986.1 PIN/TRAM domain-containing protein -
  HLI_RS10840 (HLI_11025) disA 2149667..2150740 (-) 1074 WP_128524987.1 DNA integrity scanning diadenylate cyclase DisA -
  HLI_RS10845 (HLI_11030) radA 2150741..2152120 (-) 1380 WP_128524988.1 DNA repair protein RadA Machinery gene
  HLI_RS10850 (HLI_11035) clpC 2152290..2154755 (-) 2466 WP_128524989.1 ATP-dependent protease ATP-binding subunit ClpC -
  HLI_RS10855 (HLI_11040) - 2154775..2155845 (-) 1071 WP_128524990.1 protein arginine kinase -
  HLI_RS10860 (HLI_11045) - 2155845..2156393 (-) 549 WP_128524991.1 UvrB/UvrC motif-containing protein -
  HLI_RS10865 (HLI_11050) - 2156414..2156875 (-) 462 WP_128524992.1 CtsR family transcriptional regulator -

Sequence

Protein


Download         Length: 459 a.a.        Molecular weight: 50137.62 Da        Isoelectric Point: 7.3912

>NTDB_id=267275 HLI_RS10845 WP_128524988.1 2150741..2152120(-) (radA) [Halobacillus litoralis strain ERB 031]
MAKRKTKFVCQECGYETPKWMGKCPGCQQWNTLVEEMSAPAANSRHVFQTSSTSATTKPEKITEIKSDKEPRLPTDMPEF
NRVLGGGIVAGSLVLIGGDPGIGKSTLLLQVSAQIARKDFPVLYISGEESSRQTKLRAERLKITSDDLYVLSETNLQDVS
NQIENINPKFVVIDSIQTIFKEDVTSAPGSVSQVRECTSHLMRLAKNKGIPIFIVGHVTKEGSIAGPRLLEHMVDAVLYF
EGERHHTFRILRSVKNRFGSTHEMGIFEMKEKGLEEVLNPSEIFLEERSQGAAGSIVVASMEGTRPVLVEIQALISPTAY
GNPRRMATGLDNSRVPLLMAVLEKRAGLLLQNQDAYVKVAGGVKLDEPAIDLAVAISIASSFRDQSSNGDDVVVGEVGLT
GEIRRVARIEQRVQEAAKLGFKRVIIPKKNIGGWTPPSDIEVIGVSTVQEAMKVTLGDA

Nucleotide


Download         Length: 1380 bp        

>NTDB_id=267275 HLI_RS10845 WP_128524988.1 2150741..2152120(-) (radA) [Halobacillus litoralis strain ERB 031]
TTGGCAAAAAGAAAAACAAAATTCGTTTGCCAGGAATGTGGGTATGAGACACCGAAATGGATGGGGAAATGCCCAGGGTG
TCAGCAATGGAATACACTTGTTGAAGAAATGAGTGCACCAGCGGCGAATTCACGTCATGTATTCCAGACAAGCTCCACTT
CCGCAACTACGAAACCAGAAAAAATAACAGAGATCAAATCTGATAAAGAACCCCGCTTACCTACTGACATGCCTGAATTT
AACCGCGTGCTTGGTGGTGGGATCGTGGCCGGCTCACTGGTTTTGATAGGTGGTGATCCGGGTATCGGGAAATCAACCTT
ACTCCTTCAAGTGTCTGCACAGATTGCCAGAAAGGATTTTCCAGTCTTATATATATCTGGAGAAGAATCTTCCAGGCAGA
CGAAATTGAGGGCAGAACGGTTGAAAATAACTTCAGATGACCTGTACGTATTATCAGAAACCAATCTACAAGATGTAAGT
AATCAAATTGAAAACATAAATCCGAAATTCGTCGTCATCGATTCAATCCAGACGATTTTCAAGGAAGATGTCACGTCTGC
GCCAGGGAGTGTTTCTCAAGTAAGAGAGTGTACAAGCCACTTGATGAGGCTGGCAAAAAATAAAGGGATTCCAATTTTCA
TCGTTGGGCATGTTACTAAAGAAGGCTCCATCGCGGGTCCAAGGCTGCTGGAACACATGGTGGATGCAGTTCTTTATTTT
GAAGGTGAAAGGCACCATACCTTCAGAATCTTAAGAAGTGTGAAGAACCGCTTCGGAAGTACCCATGAGATGGGCATTTT
CGAAATGAAAGAGAAAGGATTAGAAGAAGTCCTGAACCCTTCTGAAATCTTTTTAGAGGAACGGTCCCAGGGTGCGGCTG
GATCGATTGTGGTCGCTTCAATGGAAGGTACACGCCCTGTCCTGGTCGAAATCCAAGCACTTATTTCACCGACTGCTTAT
GGGAATCCCAGACGTATGGCTACCGGCTTGGATAATAGTCGTGTTCCCTTACTGATGGCCGTTTTGGAGAAAAGAGCTGG
ACTTCTTTTACAGAACCAGGATGCTTATGTGAAAGTGGCAGGAGGAGTGAAGCTGGATGAGCCTGCCATTGACTTGGCCG
TAGCGATCAGTATAGCTTCAAGCTTCCGTGATCAGTCATCAAACGGTGATGATGTCGTTGTCGGCGAAGTCGGTTTGACA
GGTGAAATCCGGAGAGTCGCAAGGATTGAACAAAGGGTACAGGAAGCGGCGAAATTAGGATTCAAGCGTGTCATCATCCC
GAAGAAGAATATAGGTGGATGGACACCGCCTTCTGATATTGAGGTGATTGGTGTCAGTACTGTACAGGAAGCGATGAAGG
TCACATTGGGGGACGCATAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(65-222)

Subunit ChlI of Mg-chelatase (ChlI)

(363-430)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A410MD79

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

72.648

99.564

0.723
  radA Streptococcus mitis NCTC 12261

62.914

98.693

0.621
  radA Streptococcus mitis SK321

62.914

98.693

0.621
  radA Streptococcus pneumoniae Rx1

62.693

98.693

0.619
  radA Streptococcus pneumoniae D39

62.693

98.693

0.619
  radA Streptococcus pneumoniae R6

62.693

98.693

0.619
  radA Streptococcus pneumoniae TIGR4

62.693

98.693

0.619

Multiple sequence alignment