Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   C2U64_RS18260 Genome accession   NZ_CP026089
Coordinates   3494222..3495022 (-) Length   266 a.a.
NCBI ID   WP_002114699.1    Uniprot ID   -
Organism   Acinetobacter pittii strain WCHAP005069     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3489222..3500022
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C2U64_RS18240 (C2U64_18230) - 3490287..3490985 (-) 699 WP_002114884.1 YfgM family protein -
  C2U64_RS18245 (C2U64_18235) hisS 3491014..3492306 (-) 1293 WP_002114994.1 histidine--tRNA ligase -
  C2U64_RS18250 (C2U64_18240) ispG 3492303..3493418 (-) 1116 WP_002115078.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  C2U64_RS18255 (C2U64_18245) - 3493440..3494231 (-) 792 WP_002114935.1 helix-turn-helix domain-containing protein -
  C2U64_RS18260 (C2U64_18250) pilF 3494222..3495022 (-) 801 WP_002114699.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  C2U64_RS18265 (C2U64_18255) rlmN 3495037..3496272 (-) 1236 WP_002115097.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  C2U64_RS18270 (C2U64_18260) ndk 3496396..3496827 (-) 432 WP_000963851.1 nucleoside-diphosphate kinase -
  C2U64_RS18275 (C2U64_18265) iscX 3496941..3497141 (-) 201 WP_002115051.1 Fe-S cluster assembly protein IscX -
  C2U64_RS18280 (C2U64_18270) - 3497241..3497792 (-) 552 WP_002114791.1 phosphatase PAP2 family protein -
  C2U64_RS18285 (C2U64_18275) - 3497817..3499106 (-) 1290 WP_079377686.1 glycosyltransferase family 4 protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30114.38 Da        Isoelectric Point: 9.0417

>NTDB_id=267028 C2U64_RS18260 WP_002114699.1 3494222..3495022(-) (pilF) [Acinetobacter pittii strain WCHAP005069]
MSIPKLKTVLCMGIAVAFLVSGCQTTHTQKKDPEKAVKVRTQLAAEHIRSGDLDSAKRALDQALSVDSRDATANMMMGIL
LQQEGSKPNLEKAEHYFKRAISSEPDNAQAHNNYGTYLYQMERYNDAVEQFRIAGATLGYDQRYQALENLGRIYLKLGDV
ANAEKTFKQALLANRDSYISMLELAEIFYLQQQIPAATQMYEQYVRTVGQKNQGARALWIGVRVARANADKMGMQVLVNQ
LRALFPESPEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=267028 C2U64_RS18260 WP_002114699.1 3494222..3495022(-) (pilF) [Acinetobacter pittii strain WCHAP005069]
TTGAGTATTCCAAAGTTAAAAACGGTTTTATGTATGGGGATTGCAGTTGCATTTCTGGTAAGTGGTTGCCAGACAACGCA
TACGCAAAAAAAAGACCCTGAGAAAGCGGTAAAGGTGCGTACACAACTTGCTGCTGAACATATCCGTTCGGGTGACTTGG
ACTCAGCAAAGCGAGCTCTTGATCAAGCTTTAAGTGTAGATAGCCGCGATGCTACTGCAAATATGATGATGGGTATTTTG
CTACAGCAAGAAGGTAGCAAACCCAATTTAGAAAAAGCAGAACATTATTTTAAAAGAGCAATTTCATCTGAACCGGATAA
TGCTCAAGCACATAATAATTATGGTACTTATTTATATCAAATGGAGCGTTATAATGATGCGGTTGAGCAATTTCGTATTG
CTGGCGCAACATTAGGTTATGACCAACGCTATCAGGCATTAGAAAATCTGGGGCGTATTTATTTAAAATTGGGTGATGTT
GCGAATGCTGAAAAAACATTCAAACAAGCACTCCTTGCTAACCGCGACTCTTATATTTCAATGTTAGAGTTAGCGGAAAT
TTTTTATTTGCAGCAGCAGATTCCGGCTGCCACACAAATGTATGAGCAATATGTTCGTACAGTGGGGCAGAAAAATCAGG
GTGCAAGAGCACTTTGGATTGGTGTAAGAGTGGCTCGAGCCAATGCGGATAAAATGGGAATGCAAGTGTTAGTAAACCAG
TTACGTGCTTTGTTCCCGGAAAGTCCAGAATATCAACGTTATTTGCAATTACAGTATAGTACTGAGGCCGTATGGAAATA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_16)

(89-134)

Tetratricopeptide repeat (TPR_16)

(149-206)

Tetratricopeptide repeat (TPR_8)

(39-68)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

93.985

100

0.94
  pilF Acinetobacter baylyi ADP1

76.154

97.744

0.744

Multiple sequence alignment