Detailed information    

insolico Bioinformatically predicted

Overview

Name   radC   Type   Machinery gene
Locus tag   DS732_RS26155 Genome accession   NZ_CP031546
Coordinates   5049285..5049953 (-) Length   222 a.a.
NCBI ID   WP_001297375.1    Uniprot ID   P65957
Organism   Escherichia coli strain cq9     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 5044285..5054953
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DS732_RS26125 (DS732_26125) rfaQ 5044463..5045521 (-) 1059 WP_000360402.1 lipopolysaccharide core heptosyltransferase RfaQ -
  DS732_RS26130 (DS732_26130) waaA 5045934..5047211 (+) 1278 WP_000891564.1 lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase -
  DS732_RS26135 (DS732_26135) coaD 5047219..5047698 (+) 480 WP_001171866.1 pantetheine-phosphate adenylyltransferase -
  DS732_RS26140 (DS732_26140) mutM 5047737..5048546 (-) 810 WP_001114533.1 bifunctional DNA-formamidopyrimidine glycosylase/DNA-(apurinic or apyrimidinic site) lyase -
  DS732_RS26145 (DS732_26145) rpmG 5048644..5048811 (-) 168 WP_001051798.1 50S ribosomal protein L33 -
  DS732_RS26150 (DS732_26150) rpmB 5048832..5049068 (-) 237 WP_000091955.1 50S ribosomal protein L28 -
  DS732_RS26155 (DS732_26155) radC 5049285..5049953 (-) 669 WP_001297375.1 DNA repair protein RadC Machinery gene
  DS732_RS26160 (DS732_26160) coaBC 5050125..5051345 (+) 1221 WP_000050158.1 bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC -
  DS732_RS26165 (DS732_26165) dut 5051323..5051781 (+) 459 WP_000976070.1 dUTP diphosphatase -
  DS732_RS26170 (DS732_26170) slmA 5051888..5052484 (+) 597 WP_000818601.1 nucleoid occlusion factor SlmA -
  DS732_RS26175 (DS732_26175) - 5052530..5053489 (-) 960 WP_000340183.1 reverse transcriptase family protein -
  DS732_RS26180 (DS732_26180) pyrE 5053787..5054428 (-) 642 WP_000806177.1 orotate phosphoribosyltransferase -

Sequence

Protein


Download         Length: 222 a.a.        Molecular weight: 25258.35 Da        Isoelectric Point: 7.9748

>NTDB_id=266310 DS732_RS26155 WP_001297375.1 5049285..5049953(-) (radC) [Escherichia coli strain cq9]
MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTLAKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQL
KGIAELARRYYNVRMREESPLLSPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAERGWI

Nucleotide


Download         Length: 669 bp        

>NTDB_id=266310 DS732_RS26155 WP_001297375.1 5049285..5049953(-) (radC) [Escherichia coli strain cq9]
GTGAAAAACAATGCACAGCTGTTGATGCCGCGCGAAAAAATGCTGAAGTTTGGTATTAGCGCCTTAACGGATGTCGAGCT
GCTGGCGCTATTTCTGCGTACCGGAACGCGCGGTAAAGATGTATTAACCCTGGCAAAAGAGATGTTGGAGAATTTCGGCT
CTCTTTATGGCTTGTTAACCTCTGAATATGAACAATTTAGTGGTGTTCATGGAATTGGCGTAGCGAAATTTGCCCAGTTA
AAGGGGATTGCTGAACTGGCGCGGCGTTACTACAACGTGCGGATGCGTGAAGAAAGCCCTTTACTCAGCCCGGAGATGAC
GCGGGAATTTTTACAAAGCCAGCTCACGGGTGAGGAGCGGGAGATCTTTATGGTGATCTTTCTCGACTCACAACACCGGG
TTATAACGCATAGCCGACTTTTTTCCGGCACGCTAAACCATGTTGAAGTCCATCCTCGGGAAATTATCCGCGAAGCGATA
AAAATAAACGCCTCGGCGCTGATCCTTGCACATAATCACCCTTCGGGTTGTGCTGAACCCAGTAAAGCGGATAAACTCAT
TACTGAACGGATAATAAAGAGTTGTCAGTTCATGGATTTACGCGTGCTCGACCATATCGTGATTGGGCGTGGAGAGTATG
TTTCTTTTGCCGAACGCGGCTGGATTTAA

Domains

Predicted by InterProScan.

RadC-like JAB domain (RadC)

(103-220)

UPF0758 N-terminal (UPF0758_N)

(9-77)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P65957

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radC Haemophilus influenzae Rd KW20

49.55

100

0.496

Multiple sequence alignment