Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   MC60_RS15230 Genome accession   NZ_CP026055
Coordinates   3356919..3358358 (+) Length   479 a.a.
NCBI ID   WP_042864830.1    Uniprot ID   -
Organism   Aeromonas caviae strain FDAARGOS_72     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3351919..3363358
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MC60_RS15200 (MC60_015200) mlaF 3352497..3353297 (-) 801 WP_029314846.1 phospholipid ABC transporter ATP-binding protein MlaF -
  MC60_RS15205 (MC60_015205) - 3353504..3354499 (+) 996 WP_042864827.1 KpsF/GutQ family sugar-phosphate isomerase -
  MC60_RS15210 (MC60_015210) kdsC 3354499..3355053 (+) 555 WP_042864828.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  MC60_RS15215 (MC60_015215) lptC 3355050..3355610 (+) 561 WP_042864829.1 LPS export ABC transporter periplasmic protein LptC -
  MC60_RS15220 (MC60_015220) lptA 3355597..3356115 (+) 519 WP_010675873.1 lipopolysaccharide transport periplasmic protein LptA -
  MC60_RS15225 (MC60_015225) lptB 3356128..3356853 (+) 726 WP_010675872.1 LPS export ABC transporter ATP-binding protein -
  MC60_RS15230 (MC60_015230) rpoN/rpoN1 3356919..3358358 (+) 1440 WP_042864830.1 RNA polymerase factor sigma-54 Machinery gene
  MC60_RS15235 (MC60_015235) hpf 3358379..3358666 (+) 288 WP_005305957.1 ribosome hibernation promoting factor -
  MC60_RS15240 (MC60_015240) ptsN 3358669..3359115 (+) 447 WP_010675870.1 PTS IIA-like nitrogen regulatory protein PtsN -
  MC60_RS15245 (MC60_015245) rapZ 3359156..3360022 (+) 867 WP_010675869.1 RNase adapter RapZ -
  MC60_RS15250 (MC60_015250) npr 3360043..3360315 (+) 273 WP_010675868.1 PTS phosphocarrier protein NPr -
  MC60_RS15255 (MC60_015255) - 3360331..3362234 (-) 1904 Protein_2937 BglG family transcription antiterminator -
  MC60_RS15260 (MC60_015260) dagF 3362338..3363078 (-) 741 WP_042864831.1 2-dehydro-3-deoxy-phosphogluconate aldolase -

Sequence

Protein


Download         Length: 479 a.a.        Molecular weight: 53775.60 Da        Isoelectric Point: 4.3728

>NTDB_id=266177 MC60_RS15230 WP_042864830.1 3356919..3358358(+) (rpoN/rpoN1) [Aeromonas caviae strain FDAARGOS_72]
MKPTLQLRLGQSLTMTPQLQQAIRLLQLSTLELQQEIQQALDNNPLLEQEENEAQETSQTETQDASQLDSSDAMAQESMP
DELPVDTTWDEVYSAGTAQSAGPIHDGEDSIYQGETTENLQDYLLWQMRLTPFSDVDAAIALAIIDAIDESGYLTATLED
IQAAVSSEEVEVELDEIEAVLKRVQHFDPVGIAARSVQECLLIQLGQYSPDLPWLDEAREVIREHMDLLGNRDYRTLARK
TRLKESDLKEVLDLIQQLEPRPGNGIIQSESQYVIPDVSVVKKGSKWVVELNPDSAPRIRVNEAYAAMARNARSSTDTQF
IRSHLQEAKWFIKSLESRNDTLLKVASCIVEQQQGFFEYGEEAMKPMVLNDIAEAVEMHESTISRVTTQKYMHTPRGIFE
LKFFFSSHVNTEGGGECSSTAIRALIKKLVTAENPAKPLSDSKIADLLAEQGIMVARRTIAKYRESLLIPPSNQRKRLV

Nucleotide


Download         Length: 1440 bp        

>NTDB_id=266177 MC60_RS15230 WP_042864830.1 3356919..3358358(+) (rpoN/rpoN1) [Aeromonas caviae strain FDAARGOS_72]
ATGAAGCCGACATTACAGTTGCGTCTCGGTCAGTCCCTGACCATGACGCCACAATTACAACAAGCGATTCGTCTGCTCCA
GCTCTCCACCCTGGAACTCCAGCAGGAAATTCAGCAAGCGCTCGACAACAACCCCTTGCTGGAGCAAGAGGAGAACGAGG
CGCAGGAAACCAGTCAGACAGAGACACAGGACGCCAGTCAACTCGACTCCAGCGATGCCATGGCCCAGGAGAGCATGCCG
GATGAACTGCCGGTGGACACCACCTGGGACGAGGTCTACTCGGCCGGTACGGCCCAGAGCGCAGGTCCCATCCATGACGG
GGAAGACAGCATCTATCAGGGGGAGACCACCGAGAACCTGCAGGACTACCTGCTGTGGCAGATGCGGCTCACCCCGTTCA
GTGATGTGGATGCTGCCATCGCCCTTGCCATCATCGATGCCATCGACGAATCCGGGTATCTGACTGCCACCCTCGAGGAT
ATTCAGGCTGCAGTCAGCAGCGAGGAGGTAGAAGTCGAACTGGATGAGATAGAAGCCGTGCTCAAGCGGGTGCAGCATTT
CGATCCGGTCGGCATCGCCGCCCGTTCAGTGCAAGAGTGTCTGCTGATCCAGCTCGGCCAGTATTCGCCGGACCTGCCCT
GGCTCGACGAAGCCAGAGAGGTGATCCGCGAACACATGGACCTGCTCGGCAATCGCGACTACCGCACCCTGGCTCGCAAG
ACCCGCCTCAAGGAGAGCGATCTCAAGGAGGTGCTGGATCTCATCCAGCAACTGGAGCCCAGACCCGGCAACGGCATCAT
CCAGAGTGAATCCCAGTACGTGATCCCGGATGTGTCCGTGGTCAAGAAGGGTAGCAAGTGGGTGGTTGAACTCAATCCGG
ATTCTGCTCCGCGCATCCGTGTCAACGAGGCCTATGCGGCCATGGCGCGCAATGCCCGCAGCAGCACGGATACCCAGTTC
ATCCGTTCTCATTTGCAGGAGGCCAAGTGGTTCATCAAGAGCCTGGAGAGTCGTAACGACACCCTGCTCAAGGTGGCCAG
CTGCATCGTCGAGCAGCAGCAGGGCTTTTTCGAGTACGGCGAAGAGGCCATGAAACCCATGGTACTCAACGACATCGCGG
AAGCGGTGGAGATGCATGAGTCGACCATTTCACGGGTCACGACCCAGAAGTACATGCATACGCCCCGCGGCATTTTTGAA
CTGAAGTTTTTTTTCTCCAGTCACGTCAATACGGAGGGAGGAGGAGAGTGCTCATCGACCGCTATTCGCGCCCTGATCAA
GAAGCTGGTGACGGCGGAAAACCCCGCCAAGCCGCTTAGTGATAGCAAGATCGCGGATTTGCTGGCCGAACAGGGTATTA
TGGTGGCGAGACGCACGATCGCAAAGTACCGTGAATCCTTGTTGATCCCGCCATCCAATCAGCGCAAACGCCTGGTTTAG

Domains

Predicted by InterProScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(319-477)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-305)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.472

100

0.482

Multiple sequence alignment