Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   C2H93_RS19520 Genome accession   NZ_CP026036
Coordinates   3812734..3814110 (+) Length   458 a.a.
NCBI ID   WP_072173577.1    Uniprot ID   -
Organism   Bacillus subtilis strain PK5_16     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 3807734..3819110
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C2H93_RS19500 (C2H93_19505) ctsR 3808087..3808551 (+) 465 WP_003225724.1 transcriptional regulator CtsR -
  C2H93_RS19505 (C2H93_19510) mcsA 3808565..3809122 (+) 558 WP_015252993.1 protein-arginine kinase activator protein McsA -
  C2H93_RS19510 (C2H93_19515) mcsB 3809122..3810213 (+) 1092 WP_003235007.1 protein arginine kinase -
  C2H93_RS19515 (C2H93_19520) clpC 3810210..3812642 (+) 2433 WP_014475592.1 ATP-dependent protease ATP-binding subunit ClpC Regulator
  C2H93_RS19520 (C2H93_19525) radA 3812734..3814110 (+) 1377 WP_072173577.1 DNA repair protein RadA Machinery gene
  C2H93_RS19525 (C2H93_19530) disA 3814114..3815196 (+) 1083 WP_003225736.1 DNA integrity scanning diadenylate cyclase DisA -
  C2H93_RS19530 (C2H93_19535) yacL 3815312..3816412 (+) 1101 WP_003235014.1 PIN/TRAM domain-containing protein -
  C2H93_RS19535 (C2H93_19540) ispD 3816427..3817125 (+) 699 WP_249852467.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  C2H93_RS19540 (C2H93_19545) ispF 3817118..3817594 (+) 477 WP_249852468.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 49422.94 Da        Isoelectric Point: 8.2399

>NTDB_id=265658 C2H93_RS19520 WP_072173577.1 3812734..3814110(+) (radA) [Bacillus subtilis strain PK5_16]
MAKTKSKFICQSCGYESPKWMGKCPGCGAWNTMVEEMIKKAPANRRAAFSHSVQTVQKPSPITSIETAEEPRVKTQLGEF
NRVLGGGVVKGSLVLIGGDPGIGKSTLLLQVSAQLSGSSNSVLYISGEESVKQTKLRADRLGINNPSLHVLSETDMEYIS
SAIQEMNPAFVVVDSIQTVYQSDITSAPGSVSQVRECTAELMKIAKTKGIPIFIVGHVTKEGSIAGPRLLEHMVDTVLYF
EGERHHTFRILRAVKNRFGSTNEMGIFEMREEGLTEVLNPSEIFLEERSAGSAGSSITASMEGTRPILVEIQALISPTSF
GNPRRMATGIDHNRVSLLMAVLEKRVGLLLQNQDAYLKVAGGVKLDEPAIDLAIAISIASSFRDTPPNPADCFIGEVGLT
GEVRRVSRIEQRVKEAAKLGFKRMIIPAANLDGWTKPKGIEVIGVANVAEALRTSLGG

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=265658 C2H93_RS19520 WP_072173577.1 3812734..3814110(+) (radA) [Bacillus subtilis strain PK5_16]
ATGGCTAAAACAAAATCGAAATTCATCTGCCAATCCTGCGGCTACGAGTCTCCAAAATGGATGGGGAAATGTCCGGGCTG
CGGTGCTTGGAATACAATGGTGGAAGAAATGATTAAAAAAGCACCGGCCAATCGGAGAGCGGCTTTTTCTCATTCTGTTC
AAACTGTACAGAAACCTTCACCTATTACATCAATCGAAACAGCAGAAGAACCCCGCGTCAAAACCCAGCTTGGCGAGTTT
AACAGAGTACTCGGCGGAGGTGTCGTTAAAGGCTCCCTCGTTTTAATTGGCGGTGATCCTGGTATCGGAAAGTCAACGCT
ATTACTGCAGGTTTCCGCTCAATTATCAGGCTCATCAAACAGTGTCCTGTATATTTCGGGAGAAGAATCTGTAAAGCAAA
CGAAGCTGCGAGCAGACCGTCTTGGCATTAATAATCCGTCACTACATGTTTTATCTGAAACCGATATGGAGTATATTTCG
TCTGCTATACAAGAGATGAATCCAGCGTTTGTCGTTGTTGACTCTATTCAAACGGTTTACCAAAGCGATATTACATCGGC
TCCAGGCAGTGTGTCACAGGTCAGAGAATGTACTGCTGAGCTGATGAAAATCGCAAAAACAAAAGGTATTCCGATTTTTA
TCGTAGGGCACGTGACGAAAGAAGGGTCTATTGCAGGTCCGAGACTGTTGGAGCATATGGTTGACACTGTTTTATATTTT
GAAGGAGAACGCCACCATACTTTCCGTATTTTGCGGGCTGTAAAAAACCGTTTCGGATCTACAAACGAAATGGGCATTTT
CGAAATGCGGGAAGAGGGGCTCACTGAGGTTTTGAATCCTTCGGAAATTTTCTTAGAAGAACGCTCTGCTGGTTCCGCAG
GCTCGAGTATCACTGCCTCTATGGAGGGCACTAGACCGATTCTCGTTGAAATTCAGGCGCTCATCTCGCCAACAAGCTTT
GGCAACCCAAGGCGTATGGCAACGGGAATAGACCATAACAGGGTTTCTCTGTTAATGGCTGTGTTAGAAAAAAGAGTAGG
GCTGCTGCTGCAAAATCAGGATGCATATTTGAAAGTGGCTGGCGGCGTTAAGCTTGATGAACCGGCAATTGATCTTGCCA
TTGCGATTAGTATCGCATCAAGCTTTAGAGACACACCTCCAAATCCTGCAGATTGCTTTATTGGAGAAGTGGGATTAACC
GGAGAAGTCCGGCGGGTTTCAAGAATTGAACAGCGTGTGAAAGAAGCGGCAAAGCTTGGCTTTAAACGGATGATCATACC
CGCGGCAAATCTGGATGGATGGACAAAACCAAAAGGGATTGAGGTTATCGGAGTAGCAAATGTTGCAGAAGCGCTTCGTA
CTTCATTAGGAGGATAA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(365-430)

AAA domain (AAA_25)

(72-222)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

99.345

100

0.993
  radA Streptococcus pneumoniae Rx1

63.355

98.908

0.627
  radA Streptococcus pneumoniae D39

63.355

98.908

0.627
  radA Streptococcus pneumoniae R6

63.355

98.908

0.627
  radA Streptococcus pneumoniae TIGR4

63.355

98.908

0.627
  radA Streptococcus mitis NCTC 12261

63.355

98.908

0.627
  radA Streptococcus mitis SK321

63.135

98.908

0.624

Multiple sequence alignment