Detailed information    

insolico Bioinformatically predicted

Overview

Name   recO   Type   Machinery gene
Locus tag   C2H96_RS19845 Genome accession   NZ_CP026035
Coordinates   3863404..3864171 (+) Length   255 a.a.
NCBI ID   WP_014477369.1    Uniprot ID   A0A0D1L7G7
Organism   Bacillus subtilis strain PK5_68     
Function   ssDNA annealing; plasmid transformation (predicted from homology)   
DNA processing Homologous recombination

Genomic Context

Location: 3858404..3869171
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C2H96_RS19815 (C2H96_19750) pgpH 3858700..3860835 (+) 2136 WP_010886570.1 cyclic-di-AMP phosphodiesterase PgpH -
  C2H96_RS19820 (C2H96_19755) ybeY 3860836..3861309 (+) 474 WP_003230039.1 rRNA maturation RNase YbeY -
  C2H96_RS19825 (C2H96_19760) unpK 3861290..3861661 (+) 372 WP_015384119.1 diacylglycerol kinase family protein -
  C2H96_RS19830 (C2H96_19765) cdd 3861780..3862190 (+) 411 WP_003230049.1 cytidine deaminase -
  C2H96_RS19835 (C2H96_19770) era 3862171..3863076 (+) 906 WP_003230051.1 GTPase Era -
  C2H96_RS19840 (C2H96_19775) yqzL 3863224..3863367 (+) 144 WP_003230053.1 YqzL family protein -
  C2H96_RS19845 (C2H96_19780) recO 3863404..3864171 (+) 768 WP_014477369.1 DNA repair protein RecO Machinery gene
  C2H96_RS19850 (C2H96_19785) glyQ 3864469..3865356 (+) 888 WP_003226213.1 glycine--tRNA ligase subunit alpha -
  C2H96_RS19855 (C2H96_19790) glyS 3865349..3867388 (+) 2040 WP_120028369.1 glycine--tRNA ligase subunit beta -
  C2H96_RS19860 (C2H96_19795) ccpN 3867521..3868159 (+) 639 WP_003237058.1 transcriptional regulator CcpN -
  C2H96_RS19865 (C2H96_19800) ppsR 3868185..3868997 (+) 813 WP_003230064.1 pyruvate, water dikinase regulatory protein -

Sequence

Protein


Download         Length: 255 a.a.        Molecular weight: 29342.05 Da        Isoelectric Point: 8.3080

>NTDB_id=265569 C2H96_RS19845 WP_014477369.1 3863404..3864171(+) (recO) [Bacillus subtilis strain PK5_68]
MLTKCEGIVLRTNDYGETNKIVTLLTREHGKIGVMARGAKKPNSRLSAVSQPFLYGSFLMQKTSGLGTLQQGEMILSMRG
IREDLFLTAYAAYIAELVDRGTEEKKPNPYLFEFILESLKQLNEGTDPDVITFIVQMKMLGVMGLYPELNHCVHCKSQDG
TFHFSVRDNGFICHRCFEKDPYRIPIKPQTARLLRLFYYFDLSRLGNVSLKEETKAELKQVIDLYYEEYSGLYLKSKRFL
DQMESMKHLMGENKS

Nucleotide


Download         Length: 768 bp        

>NTDB_id=265569 C2H96_RS19845 WP_014477369.1 3863404..3864171(+) (recO) [Bacillus subtilis strain PK5_68]
ATGCTGACAAAATGTGAAGGAATCGTTCTTCGCACAAATGATTACGGAGAGACGAATAAAATCGTAACATTACTGACAAG
AGAACACGGAAAAATAGGTGTTATGGCACGAGGCGCCAAAAAACCGAACAGCCGTTTATCAGCAGTCAGCCAGCCCTTTT
TGTATGGTTCTTTCTTAATGCAAAAAACATCGGGCCTCGGAACGCTTCAGCAGGGGGAAATGATCCTCAGCATGAGAGGC
ATCAGGGAAGACTTGTTTTTAACAGCATATGCTGCCTATATTGCTGAATTAGTCGACAGAGGCACAGAAGAAAAAAAGCC
AAACCCTTATTTATTTGAATTCATATTAGAATCATTAAAACAGCTGAATGAAGGAACAGATCCGGATGTTATCACCTTCA
TTGTTCAAATGAAGATGCTCGGTGTCATGGGCCTGTATCCTGAGCTAAATCATTGTGTACATTGTAAAAGCCAAGACGGA
ACCTTTCATTTCTCTGTTCGTGATAATGGTTTTATATGCCACCGCTGTTTTGAAAAAGATCCTTACAGGATACCGATTAA
ACCGCAAACGGCAAGGCTGTTAAGACTCTTTTACTATTTTGATCTGTCGAGGCTCGGCAATGTGTCCTTAAAAGAGGAAA
CAAAAGCTGAATTAAAGCAAGTGATTGATTTATATTACGAAGAATATTCGGGATTGTATTTAAAATCAAAGCGCTTTTTA
GATCAAATGGAAAGCATGAAACATCTTATGGGTGAAAACAAAAGTTGA

Domains

Predicted by InterproScan.

Recombination protein O N terminal (RecO_N)

(1-75)

Recombination protein O C terminal (RecO_C)

(82-237)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0D1L7G7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recO Bacillus subtilis subsp. subtilis str. 168

99.608

100

0.996

Multiple sequence alignment