Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   C2H91_RS17960 Genome accession   NZ_CP026030
Coordinates   3591403..3591813 (+) Length   136 a.a.
NCBI ID   WP_009967785.1    Uniprot ID   A0A6I4D881
Organism   Bacillus subtilis strain PK3_9     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 3586403..3596813
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C2H91_RS17925 (C2H91_17950) yrkP 3587079..3587774 (+) 696 WP_021480140.1 two-component response regulator YrkP -
  C2H91_RS17930 (C2H91_17955) yrkQ 3587761..3589059 (+) 1299 WP_072174302.1 two-component system sensor histidine kinase YrkQ -
  C2H91_RS17935 (C2H91_17960) psiE 3589105..3589521 (+) 417 WP_003246154.1 phosphate-starvation-inducible protein PsiE -
  C2H91_RS17940 (C2H91_17965) - 3590065..3590243 (+) 179 Protein_3482 hypothetical protein -
  C2H91_RS17945 (C2H91_17970) - 3590201..3590377 (+) 177 Protein_3483 recombinase family protein -
  C2H91_RS17950 - 3590364..3590479 (-) 116 Protein_3484 hypothetical protein -
  C2H91_RS17955 (C2H91_17975) sigK 3590479..3591207 (-) 729 WP_013308023.1 RNA polymerase sporulation sigma factor SigK -
  C2H91_RS17960 (C2H91_17980) nucA/comI 3591403..3591813 (+) 411 WP_009967785.1 sporulation-specific Dnase NucB Machinery gene
  C2H91_RS17965 (C2H91_17985) yqeB 3591846..3592568 (-) 723 WP_249850930.1 DUF308 domain-containing protein -
  C2H91_RS17970 (C2H91_17990) gnd 3592820..3593713 (+) 894 WP_041850033.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  C2H91_RS17975 (C2H91_17995) yqeD 3593733..3594359 (-) 627 WP_021480068.1 TVP38/TMEM64 family protein -
  C2H91_RS17980 (C2H91_18000) cwlH 3594546..3595298 (+) 753 WP_124072948.1 N-acetylmuramoyl-L-alanine amidase CwlH -
  C2H91_RS17985 (C2H91_18005) yqeF 3595550..3596281 (+) 732 WP_249850931.1 SGNH/GDSL hydrolase family protein -
  C2H91_RS17990 (C2H91_18010) - 3596587..3596727 (-) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14967.97 Da        Isoelectric Point: 5.1853

>NTDB_id=265299 C2H91_RS17960 WP_009967785.1 3591403..3591813(+) (nucA/comI) [Bacillus subtilis strain PK3_9]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=265299 C2H91_RS17960 WP_009967785.1 3591403..3591813(+) (nucA/comI) [Bacillus subtilis strain PK3_9]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAACAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGAGATGCAATTGCAG
AGGGACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACC
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCGGTCTGCGAGGAAGGCGGCGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTATCCTGACGGTACCAGAGTGCTGTTTA
TTGTGCAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(40-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6I4D881

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

62.609

84.559

0.529

Multiple sequence alignment