Detailed information NTG0026411

Bioinformatically predicted

Overview

• Name: pilA
• Type: Machinery gene
• Locus tag: BAMB_RS14495
• Genome accession: NC_008390
• Coordinates: 3162001..3163161 (+)
• Length: 386 a.a.
• NCBI ID: WP_011657978.1
• Uniprot ID: Q0BBP8
• Organism: Burkholderia ambifaria AMMD
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3157001..3168161

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BAMB_RS14465 (Bamb_2863)	-	3157615..3158223 (+)	609	WP_006753282.1	50S ribosomal protein L25/general stress protein Ctc	-
BAMB_RS14470 (Bamb_2864)	pth	3158353..3158952 (+)	600	WP_006760396.1	aminoacyl-tRNA hydrolase	-
BAMB_RS14475 (Bamb_2865)	hisC	3159078..3160145 (+)	1068	WP_011657975.1	histidinol-phosphate transaminase	-
BAMB_RS14480 (Bamb_2866)	-	3160250..3160516 (-)	267	WP_011657976.1	YfhL family 4Fe-4S dicluster ferredoxin	-
BAMB_RS14485 (Bamb_2867)	coaD	3160576..3161073 (-)	498	WP_006398238.1	pantetheine-phosphate adenylyltransferase	-
BAMB_RS14490 (Bamb_2868)	rsmD	3161185..3161799 (-)	615	WP_041491281.1	16S rRNA (guanine(966)-N(2))-methyltransferase RsmD	-
BAMB_RS14495 (Bamb_2869)	pilA	3162001..3163161 (+)	1161	WP_011657978.1	signal recognition particle-docking protein FtsY	Machinery gene
BAMB_RS14500 (Bamb_2870)	maiA	3163271..3163915 (-)	645	WP_041491400.1	maleylacetoacetate isomerase	-
BAMB_RS14505 (Bamb_2871)	ybiB	3164105..3165079 (+)	975	WP_041491401.1	DNA-binding protein YbiB	-
BAMB_RS14510 (Bamb_2872)	leuA	3165470..3167116 (+)	1647	WP_011657981.1	2-isopropylmalate synthase	-
BAMB_RS14515 (Bamb_2873)	-	3167260..3167643 (-)	384	WP_011657982.1	nuclear transport factor 2 family protein	-

Sequence

Protein

• Length: 386 a.a.
• Molecular weight: 41170.99 Da
• Isoelectric Point: 4.8019

>NTDB_id=26411 BAMB_RS14495 WP_011657978.1 3162001..3163161(+) (pilA) [Burkholderia ambifaria AMMD]
MFSFFKRFKKTQESDSTESQSADALQPDEPSDAPAADARQAIEAPPAPAQPQPAAPAVVMTVTPTNDGSDDVVEAVEIVP
PPTQDASAKKSWLTRLKTGLAKTGSSITGVFVNTKIDEDLYEELEAALLMSDAGVDATEYLLGALREKVKTGRLTDPQQV
KSALHDLLVELLKPLEKSLMLGRAQPLVMMITGVNGAGKTTSIGKLAKHLQSFDQSVLLAAGDTFRAAAREQLAVWGERN
NVTVVQQESGDPAAVIFDAVSAARARKIDVMMADTAGRLPTQLHLMEELKKVKRVISKAHDGAPHEVLLVIDANTGQNAL
TQVKAFDDALGLTGLIVTKLDGTAKGGILAAIARQRPVPVYFIGVGEKVEDLQPFNAVEFADALFD

Nucleotide

• Length: 1161 bp

>NTDB_id=26411 BAMB_RS14495 WP_011657978.1 3162001..3163161(+) (pilA) [Burkholderia ambifaria AMMD]
ATGTTCAGCTTCTTCAAACGATTCAAGAAAACGCAGGAGTCCGACTCCACGGAATCGCAATCGGCCGACGCGCTGCAACC
GGACGAGCCGTCCGATGCGCCCGCCGCCGATGCCCGTCAGGCGATCGAAGCCCCGCCAGCCCCCGCGCAACCGCAACCGG
CCGCGCCGGCCGTCGTGATGACGGTCACGCCGACCAACGACGGCAGCGACGACGTCGTCGAAGCGGTCGAGATCGTGCCG
CCGCCGACGCAGGACGCCTCCGCGAAGAAATCGTGGCTCACCCGCCTGAAAACGGGGCTCGCGAAGACGGGTTCGAGCAT
CACCGGCGTGTTCGTCAACACGAAGATCGACGAGGATCTGTACGAAGAACTCGAAGCCGCGCTGCTGATGTCCGACGCCG
GCGTCGATGCGACCGAGTACCTGCTCGGTGCGCTGCGCGAGAAAGTGAAAACGGGCCGGCTGACCGATCCGCAGCAGGTG
AAGAGCGCGCTGCACGATCTGCTCGTCGAACTGCTGAAGCCGCTCGAGAAATCGCTGATGCTCGGCCGCGCGCAGCCGCT
CGTGATGATGATCACGGGCGTGAACGGCGCGGGCAAGACCACCAGCATCGGCAAGCTCGCGAAGCATCTGCAGAGCTTCG
ACCAATCGGTGCTGCTGGCCGCGGGCGACACGTTCCGCGCGGCCGCGCGCGAACAGCTGGCGGTCTGGGGCGAGCGCAAC
AACGTGACGGTCGTCCAGCAGGAAAGCGGCGATCCGGCCGCGGTGATCTTCGACGCGGTCAGCGCCGCGCGCGCGCGCAA
GATCGACGTGATGATGGCCGACACGGCCGGCCGCCTGCCGACGCAGCTGCACCTGATGGAAGAGCTGAAGAAGGTGAAGC
GCGTGATCTCGAAGGCGCACGACGGCGCGCCGCACGAGGTGCTGCTCGTGATCGACGCGAACACCGGCCAGAACGCGCTC
ACGCAGGTGAAGGCCTTCGACGATGCGCTCGGCCTCACGGGCCTGATCGTCACGAAGCTCGACGGCACCGCCAAGGGCGG
GATTCTGGCGGCGATCGCGCGGCAGCGCCCGGTGCCCGTCTACTTCATCGGCGTCGGCGAGAAGGTCGAGGACCTGCAGC
CGTTCAACGCCGTGGAATTCGCGGACGCGCTATTCGACTGA

Domains

Predicted by InterproScan.

• SRP54-type protein, GTPase domain (SRP54) (187-385)

• SRP54-type protein, helical bundle domain (SRP54_N) (104-166)

3D structure

• AlphaFold DB: Q0BBP8

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilA	Neisseria gonorrhoeae MS11	52.632	100	0.57