Detailed information    

insolico Bioinformatically predicted

Overview

Name   radC   Type   Machinery gene
Locus tag   DWB25_RS00710 Genome accession   NZ_CP031293
Coordinates   144854..145522 (+) Length   222 a.a.
NCBI ID   WP_001297375.1    Uniprot ID   P65957
Organism   Escherichia coli strain EC17GD31     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 139854..150522
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DWB25_RS00680 (DWB25_00720) yicC 139873..140736 (-) 864 WP_000621328.1 YicC/YloC family endoribonuclease -
  DWB25_RS00685 (DWB25_00730) rph 140863..141579 (+) 717 WP_001247093.1 ribonuclease PH -
  DWB25_RS00690 (DWB25_00735) pyrE 141645..142286 (+) 642 WP_000806177.1 orotate phosphoribosyltransferase -
  DWB25_RS00695 (DWB25_00740) slmA 142323..142919 (-) 597 WP_000818603.1 nucleoid occlusion factor SlmA -
  DWB25_RS00700 (DWB25_00745) dut 143026..143484 (-) 459 WP_000976070.1 dUTP diphosphatase -
  DWB25_RS00705 (DWB25_00750) coaBC 143462..144682 (-) 1221 WP_000050149.1 bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC -
  DWB25_RS00710 (DWB25_00755) radC 144854..145522 (+) 669 WP_001297375.1 DNA repair protein RadC Machinery gene
  DWB25_RS00715 (DWB25_00760) rpmB 145739..145975 (+) 237 WP_000091955.1 50S ribosomal protein L28 -
  DWB25_RS00720 (DWB25_00765) rpmG 145996..146163 (+) 168 WP_001051798.1 50S ribosomal protein L33 -
  DWB25_RS00725 (DWB25_00770) mutM 146261..147070 (+) 810 WP_001114533.1 bifunctional DNA-formamidopyrimidine glycosylase/DNA-(apurinic or apyrimidinic site) lyase -
  DWB25_RS00730 (DWB25_00775) coaD 147109..147588 (-) 480 WP_001171866.1 pantetheine-phosphate adenylyltransferase -
  DWB25_RS00735 (DWB25_00780) waaA 147596..148873 (-) 1278 WP_000891574.1 lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase -
  DWB25_RS00740 (DWB25_00785) rfaQ 149286..150344 (+) 1059 WP_000360395.1 lipopolysaccharide core heptosyltransferase RfaQ -

Sequence

Protein


Download         Length: 222 a.a.        Molecular weight: 25258.35 Da        Isoelectric Point: 7.9748

>NTDB_id=264004 DWB25_RS00710 WP_001297375.1 144854..145522(+) (radC) [Escherichia coli strain EC17GD31]
MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTLAKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQL
KGIAELARRYYNVRMREESPLLSPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAERGWI

Nucleotide


Download         Length: 669 bp        

>NTDB_id=264004 DWB25_RS00710 WP_001297375.1 144854..145522(+) (radC) [Escherichia coli strain EC17GD31]
GTGAAAAACAATGCACAGCTGTTGATGCCGCGCGAAAAAATGCTGAAGTTTGGTATTAGCGCCTTAACGGATGTCGAGCT
GCTGGCGCTATTTCTGCGTACCGGAACGCGCGGTAAAGATGTATTAACCCTGGCAAAAGAGATGCTGGAGAATTTCGGCT
CTCTTTATGGCTTGTTAACCTCTGAATATGAACAGTTTAGTGGCGTTCATGGAATTGGCGTGGCGAAATTCGCCCAGTTA
AAGGGGATTGCCGAACTGGCGCGGCGTTACTACAACGTGCGGATGCGTGAAGAAAGCCCTTTACTCAGCCCGGAGATGAC
GAGGGAATTTTTACAAAGCCAGCTTACGGGCGAGGAGCGGGAGATCTTTATGGTGATCTTTCTCGACTCACAACACCGGG
TTATAACGCATAGCCGTCTTTTTTCCGGCACGCTAAACCATGTTGAAGTCCATCCTCGGGAAATTATCCGCGAAGCGATA
AAAATAAATGCCTCGGCGCTGATCCTTGCACATAATCACCCTTCGGGTTGTGCTGAACCCAGTAAAGCGGATAAACTCAT
TACTGAACGGATAATAAAGAGTTGTCAGTTCATGGATTTACGCGTGCTCGACCATATCGTGATTGGGCGTGGAGAGTATG
TTTCTTTTGCCGAACGCGGCTGGATTTAA

Domains

Predicted by InterProScan.

RadC-like JAB domain (RadC)

(103-220)

UPF0758 N-terminal (UPF0758_N)

(9-77)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P65957

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radC Haemophilus influenzae Rd KW20

49.55

100

0.496

Multiple sequence alignment