Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   H16_RS10545 Genome accession   NC_008313
Coordinates   2297742..2299103 (-) Length   453 a.a.
NCBI ID   WP_010810633.1    Uniprot ID   A0A367PNZ5
Organism   Cupriavidus necator H16     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2292742..2304103
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H16_RS10520 (H16_A2107) - 2293517..2294173 (-) 657 WP_037024914.1 sugar transferase -
  H16_RS35105 (H16_A2108) - 2294445..2294747 (+) 303 WP_011615497.1 hypothetical protein -
  H16_RS10525 (H16_A2109) - 2294755..2296179 (+) 1425 WP_231881346.1 NAD(P)-binding domain-containing protein -
  H16_RS34250 - 2296248..2296424 (+) 177 Protein_2143 transposase -
  H16_RS10535 (H16_A2110) - 2296538..2297023 (-) 486 WP_010810635.1 disulfide bond formation protein B -
  H16_RS10540 (H16_A2111) - 2297181..2297738 (-) 558 Protein_2145 GNAT family N-acetyltransferase -
  H16_RS10545 (H16_A2112) radA 2297742..2299103 (-) 1362 WP_010810633.1 DNA repair protein RadA Machinery gene
  H16_RS10550 (H16_A2113) alr 2299199..2300320 (-) 1122 WP_010810632.1 alanine racemase -
  H16_RS10555 (H16_A2114) lplT 2300894..2302213 (+) 1320 WP_010810631.1 lysophospholipid transporter LplT -
  H16_RS10560 (H16_A2115) - 2302229..2303365 (-) 1137 WP_011615501.1 DUF1853 family protein -

Sequence

Protein


Download         Length: 453 a.a.        Molecular weight: 48373.69 Da        Isoelectric Point: 7.2389

>NTDB_id=26239 H16_RS10545 WP_010810633.1 2297742..2299103(-) (radA) [Cupriavidus necator H16]
MAKTKTVYTCTECGGTTPRWAGQCPHCQQWNTLVETVAESAANKRFQPLAASATVRKLSEIDAADVPRFSSGIDEFDRVL
GGGLVSGGVVLIGGDPGIGKSTLLLQALANLAGQRRVLYVSGEESGAQIALRAQRLGVESPSLGLLAEIQLEKIQATLEV
EKPEVAVIDSIQTLYSEALTSAPGSVAQVRECAAQLTRIAKSSGITIILVGHVTKEGSLAGPRVLEHIVDTVLYFEGDTH
SSHRLIRAFKNRFGAVNELGVFAMTERGLRGISNPSALFLSQHEETVPGSCVLVTQEGTRPLLVEIQALVDTAHVPNPRR
LAVGLEQNRLALLLAVLHRHAGIACFDQDVFLNAVGGVKITEPAADLAVLLSIHSSMRNKPLPRGLVVFGEVGLAGEIRP
SPRGQERLKEAAKLGFTIAVIPKANAPKQKIDGLEVIAVERIEQAIDRVRHLD

Nucleotide


Download         Length: 1362 bp        

>NTDB_id=26239 H16_RS10545 WP_010810633.1 2297742..2299103(-) (radA) [Cupriavidus necator H16]
TTGGCCAAGACCAAGACTGTCTATACATGTACTGAATGCGGCGGCACCACGCCGCGCTGGGCCGGCCAGTGCCCGCATTG
CCAGCAATGGAACACGCTGGTGGAGACGGTGGCCGAGTCCGCCGCGAACAAGCGCTTCCAGCCGCTGGCGGCCTCGGCCA
CGGTGCGCAAGCTGTCGGAGATCGATGCCGCCGACGTGCCGCGCTTTTCCAGCGGCATCGATGAATTCGACCGCGTGCTG
GGCGGCGGCCTGGTGTCGGGCGGGGTGGTGCTGATCGGCGGCGATCCCGGCATCGGCAAGTCCACGCTGCTGCTGCAGGC
ACTGGCCAACCTTGCGGGCCAGCGCCGCGTGCTCTACGTCAGCGGCGAAGAATCCGGTGCGCAGATCGCGCTGCGCGCGC
AGCGGCTGGGCGTGGAAAGCCCGTCACTGGGGCTGCTCGCCGAAATCCAGCTGGAGAAGATCCAGGCCACGCTGGAAGTG
GAAAAGCCCGAAGTCGCGGTGATCGACTCGATCCAGACGCTGTACTCCGAGGCGTTGACCTCCGCGCCGGGCTCGGTCGC
GCAGGTGCGCGAATGCGCGGCGCAGCTGACGCGCATTGCCAAGAGCAGCGGCATCACCATCATCCTGGTCGGCCACGTGA
CCAAGGAAGGCAGCCTGGCCGGTCCGCGCGTGCTGGAGCATATCGTCGATACGGTGCTGTATTTCGAGGGCGATACCCAT
TCCTCGCACCGGCTGATCCGCGCCTTCAAGAACCGCTTCGGCGCGGTCAATGAGCTGGGCGTGTTTGCCATGACCGAGCG
CGGCCTGCGCGGCATCAGCAATCCGTCGGCGCTGTTCCTGTCGCAGCACGAAGAGACCGTGCCGGGCTCATGCGTGCTGG
TGACGCAGGAGGGCACGCGTCCGCTGCTGGTGGAGATCCAGGCGCTGGTCGATACCGCCCATGTGCCCAACCCGCGCCGG
CTGGCGGTGGGCCTTGAGCAGAACCGGCTGGCGCTGCTGCTGGCAGTGCTGCACCGGCACGCGGGCATAGCCTGCTTTGA
CCAGGACGTGTTCCTCAACGCTGTGGGCGGGGTCAAGATCACCGAACCCGCCGCCGATCTCGCGGTGCTGCTGTCGATCC
ACTCGTCGATGCGCAACAAGCCGCTGCCGCGCGGCCTGGTGGTGTTCGGCGAAGTCGGCCTGGCCGGCGAGATCCGGCCC
AGCCCGCGCGGGCAGGAGCGTCTGAAGGAAGCCGCCAAGCTGGGCTTTACGATCGCCGTGATTCCTAAGGCGAACGCGCC
CAAACAGAAGATCGACGGGCTCGAAGTGATCGCGGTGGAACGCATCGAGCAGGCCATCGACCGCGTGCGCCATCTGGACT
GA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(63-217)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A367PNZ5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

51.327

99.779

0.512
  radA Streptococcus pneumoniae Rx1

46.813

100

0.47
  radA Streptococcus pneumoniae D39

46.813

100

0.47
  radA Streptococcus pneumoniae R6

46.813

100

0.47
  radA Streptococcus pneumoniae TIGR4

46.813

100

0.47
  radA Streptococcus mitis SK321

46.916

100

0.47
  radA Streptococcus mitis NCTC 12261

46.593

100

0.468

Multiple sequence alignment