Detailed information NTG0026230

Bioinformatically predicted

Overview

• Name: pilA
• Type: Machinery gene
• Locus tag: H16_RS01785
• Genome accession: NC_008313
• Coordinates: 380549..381709 (-)
• Length: 386 a.a.
• NCBI ID: WP_011614476.1
• Uniprot ID: Q0KEQ7
• Organism: Cupriavidus necator H16
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 375549..386709

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
H16_RS01760 (H16_A0358)	ntrB	375644..376549 (-)	906	WP_010814805.1	nitrate ABC transporter permease	-
H16_RS01765 (H16_A0359)	-	376601..377911 (-)	1311	WP_010814804.1	CmpA/NrtA family ABC transporter substrate-binding protein	-
H16_RS01770 (H16_A0360)	-	378277..378924 (-)	648	WP_037025353.1	ANTAR domain-containing response regulator	-
H16_RS01775 (H16_A0361)	-	379178..379879 (+)	702	WP_011614475.1	fumarylacetoacetate hydrolase family protein	-
H16_RS01780 (H16_A0362)	maiA	379884..380531 (+)	648	WP_010814801.1	maleylacetoacetate isomerase	-
H16_RS01785 (H16_A0363)	pilA	380549..381709 (-)	1161	WP_011614476.1	signal recognition particle-docking protein FtsY	Machinery gene
H16_RS01790 (H16_A0364)	-	381928..383475 (+)	1548	WP_010814799.1	M16 family metallopeptidase	-
H16_RS01795 (H16_A0365)	-	383485..384867 (+)	1383	WP_010814798.1	M16 family metallopeptidase	-
H16_RS01800 (H16_A0366)	rsmD	384950..385642 (+)	693	WP_011614477.1	16S rRNA (guanine(966)-N(2))-methyltransferase RsmD	-
H16_RS01805 (H16_A0367)	coaD	385883..386368 (+)	486	WP_010814369.1	pantetheine-phosphate adenylyltransferase	-
H16_RS01810 (H16_A0368)	-	386437..386697 (+)	261	WP_010814370.1	YfhL family 4Fe-4S dicluster ferredoxin	-

Sequence

Protein

• Length: 386 a.a.
• Molecular weight: 40868.63 Da
• Isoelectric Point: 7.3625

>NTDB_id=26230 H16_RS01785 WP_011614476.1 380549..381709(-) (pilA) [Cupriavidus necator H16]
MFSFWKKRKAEPAPAEAPAPAPAPVEVPAPVPAPAPTPAPVPAPAPAPVPAQVPPPAPAPVAAPIPLPAPAPAEALELVP
PPAQTAEAKLGWMHRLRTGLSKTSRNIGTLFVGVKVDEALFEELETALLMADAGVEATEYLLGELRKRVKAERIETAEGV
KAALRELLTQLLRPLEKTMALGREQPLVMMIAGVNGAGKTTSIGKLCKHFQRYDQKVLLAAGDTFRAAAREQLTIWGERN
NVTVVAQESGDPAAVIFDAVNAAKARGIDIVMADTAGRLPTQLHLMEELKKVKRVISKAMPSAPHEVLLVIDANTGQNAL
QQTRAFDDALGLTGLIVTKLDGTAKGGILAAIARQRPVPVYFIGVGEKVEDLQPFKADEFAEALLG

Nucleotide

• Length: 1161 bp

>NTDB_id=26230 H16_RS01785 WP_011614476.1 380549..381709(-) (pilA) [Cupriavidus necator H16]
ATGTTTAGTTTCTGGAAGAAGCGCAAGGCCGAGCCCGCGCCCGCCGAGGCGCCAGCCCCGGCCCCCGCTCCGGTCGAGGT
CCCCGCCCCCGTACCGGCACCGGCCCCCACGCCTGCGCCAGTCCCTGCGCCCGCACCGGCACCGGTCCCGGCGCAAGTGC
CACCCCCTGCCCCAGCGCCCGTGGCGGCCCCGATCCCCCTGCCGGCGCCCGCACCGGCCGAAGCGCTCGAGCTGGTCCCG
CCGCCCGCCCAGACCGCTGAAGCGAAGCTGGGCTGGATGCATCGGCTGCGTACCGGGCTGTCCAAGACCAGCCGCAACAT
CGGCACGCTGTTCGTCGGCGTCAAGGTCGACGAGGCCCTGTTCGAAGAACTGGAAACCGCGCTGCTGATGGCAGATGCCG
GCGTGGAAGCCACCGAGTACCTGCTGGGCGAGTTGCGCAAGCGCGTCAAGGCCGAGCGCATCGAGACCGCCGAAGGCGTC
AAGGCGGCGCTGCGCGAGCTGCTGACGCAGCTGCTGCGCCCGCTGGAAAAAACCATGGCGCTGGGCCGCGAGCAGCCGCT
GGTGATGATGATCGCGGGCGTCAACGGCGCCGGCAAGACCACCAGCATCGGCAAGCTGTGCAAGCACTTCCAGCGCTATG
ACCAGAAGGTGCTGCTGGCCGCCGGCGATACCTTCCGCGCCGCCGCGCGCGAGCAACTGACGATCTGGGGCGAACGCAAC
AACGTCACCGTGGTGGCGCAGGAAAGCGGCGACCCGGCGGCGGTGATCTTCGACGCCGTCAATGCGGCCAAGGCGCGCGG
CATCGACATCGTCATGGCCGACACCGCCGGCCGCCTGCCGACGCAGCTGCACCTGATGGAAGAGCTCAAGAAGGTCAAGC
GCGTGATCAGCAAGGCCATGCCGAGCGCGCCGCACGAGGTGCTGCTGGTGATCGACGCCAACACCGGCCAGAACGCCCTG
CAGCAAACCCGCGCCTTCGACGACGCGCTGGGCCTGACCGGCCTGATCGTGACCAAGCTCGACGGCACCGCCAAGGGCGG
CATCCTGGCGGCTATCGCGCGCCAGCGCCCGGTGCCGGTTTATTTCATTGGCGTGGGCGAGAAGGTGGAGGACCTGCAGC
CGTTCAAGGCGGATGAGTTCGCCGAAGCGCTGCTGGGGTAA

Domains

Predicted by InterproScan.

• SRP54-type protein, GTPase domain (SRP54) (187-386)

• SRP54-type protein, helical bundle domain (SRP54_N) (111-166)

3D structure

• AlphaFold DB: Q0KEQ7

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilA	Neisseria gonorrhoeae MS11	51.202	100	0.552