Detailed information    

insolico Bioinformatically predicted

Overview

Name   radC   Type   Machinery gene
Locus tag   DTO54_RS00380 Genome accession   NZ_CP030939
Coordinates   71998..72666 (+) Length   222 a.a.
NCBI ID   WP_001298959.1    Uniprot ID   Q31UY8
Organism   Escherichia coli strain AMSHJX01     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 66998..77666
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DTO54_RS00345 (DTO54_00345) yicC 67017..67880 (-) 864 WP_000621336.1 YicC/YloC family endoribonuclease -
  DTO54_RS00355 (DTO54_00355) rph 68007..68723 (+) 717 WP_001247093.1 ribonuclease PH -
  DTO54_RS00360 (DTO54_00360) pyrE 68789..69430 (+) 642 WP_000806162.1 orotate phosphoribosyltransferase -
  DTO54_RS00365 (DTO54_00365) slmA 69467..70063 (-) 597 WP_000818601.1 nucleoid occlusion factor SlmA -
  DTO54_RS00370 (DTO54_00370) dut 70170..70628 (-) 459 WP_000976070.1 dUTP diphosphatase -
  DTO54_RS00375 (DTO54_00375) coaBC 70606..71826 (-) 1221 WP_000050139.1 bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC -
  DTO54_RS00380 (DTO54_00380) radC 71998..72666 (+) 669 WP_001298959.1 DNA repair protein RadC Machinery gene
  DTO54_RS00385 (DTO54_00385) rpmB 72883..73119 (+) 237 WP_000091955.1 50S ribosomal protein L28 -
  DTO54_RS00390 (DTO54_00390) rpmG 73140..73307 (+) 168 WP_001051798.1 50S ribosomal protein L33 -
  DTO54_RS00395 (DTO54_00395) mutM 73405..74214 (+) 810 WP_001114546.1 bifunctional DNA-formamidopyrimidine glycosylase/DNA-(apurinic or apyrimidinic site) lyase -
  DTO54_RS00400 (DTO54_00400) coaD 74253..74732 (-) 480 WP_001171866.1 pantetheine-phosphate adenylyltransferase -
  DTO54_RS00405 (DTO54_00405) waaA 74740..76017 (-) 1278 WP_000891564.1 lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase -
  DTO54_RS00410 (DTO54_00410) rfaQ 76430..77488 (+) 1059 WP_000360412.1 lipopolysaccharide core heptosyltransferase RfaQ -

Sequence

Protein


Download         Length: 222 a.a.        Molecular weight: 25274.35 Da        Isoelectric Point: 7.9748

>NTDB_id=261949 DTO54_RS00380 WP_001298959.1 71998..72666(+) (radC) [Escherichia coli strain AMSHJX01]
MKNNSQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTLAKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQL
KGIAELARRYYNVRMREESPLLSPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAERGWI

Nucleotide


Download         Length: 669 bp        

>NTDB_id=261949 DTO54_RS00380 WP_001298959.1 71998..72666(+) (radC) [Escherichia coli strain AMSHJX01]
GTGAAAAACAATTCACAGCTGTTGATGCCGCGCGAAAAAATGCTGAAGTTTGGTATTAGCGCCTTAACGGATGTCGAGCT
GCTGGCGCTATTTCTGCGTACCGGAACGCGCGGTAAAGATGTATTAACCCTGGCAAAAGAGATGCTGGAGAATTTCGGCT
CTCTTTATGGCTTGTTAACCTCTGAATATGAACAATTTAGTGGCGTTCATGGAATTGGCGTGGCGAAATTTGCCCAGTTA
AAGGGGATTGCTGAACTGGCGCGGCGTTACTACAACGTGCGGATGCGTGAAGAAAGCCCTTTACTCAGCCCGGAGATGAC
GAGGGAATTTTTACAAAGCCAGCTCACGGGTGAGGAGCGGGAGATCTTTATGGTGATCTTTCTCGACTCCCAACACCGGG
TTATAACGCATAGCCGTCTTTTTTCCGGCACGCTAAACCATGTTGAAGTCCATCCTCGGGAAATTATCCGCGAAGCGATA
AAAATAAACGCCTCGGCGCTGATCCTTGCACATAATCACCCTTCGGGTTGTGCTGAACCCAGTAAAGCGGATAAACTCAT
TACTGAACGGATAATAAAGAGTTGTCAGTTCATGGATTTACGCGTGCTCGACCATATCGTGATTGGGCGTGGAGAGTATG
TTTCTTTTGCCGAACGCGGCTGGATTTAA

Domains

Predicted by InterProScan.

UPF0758 N-terminal (UPF0758_N)

(9-77)

RadC-like JAB domain (RadC)

(103-220)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q31UY8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radC Haemophilus influenzae Rd KW20

49.55

100

0.496