Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   CYK59_RS04555 Genome accession   NZ_CP025476
Coordinates   840448..841119 (+) Length   223 a.a.
NCBI ID   WP_004270751.1    Uniprot ID   A0A1B2A7J9
Organism   Latilactobacillus curvatus strain IRG2     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 835448..846119
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CYK59_RS04525 (CYK59_04545) - 835659..836846 (+) 1188 WP_004270744.1 FtsW/RodA/SpoVE family cell cycle protein -
  CYK59_RS04530 (CYK59_04550) - 836859..837947 (+) 1089 WP_126133317.1 CAP-associated domain-containing protein -
  CYK59_RS04535 (CYK59_04555) - 837967..838257 (+) 291 WP_004270748.1 YlbG family protein -
  CYK59_RS04540 (CYK59_04560) rsmD 838263..838817 (+) 555 WP_004270750.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  CYK59_RS04545 (CYK59_04565) coaD 838829..839323 (+) 495 WP_065825725.1 pantetheine-phosphate adenylyltransferase -
  CYK59_RS04550 (CYK59_04570) - 839325..840371 (+) 1047 WP_080563410.1 SepM family pheromone-processing serine protease -
  CYK59_RS04555 (CYK59_04575) comEA 840448..841119 (+) 672 WP_004270751.1 helix-hairpin-helix domain-containing protein Machinery gene
  CYK59_RS04560 (CYK59_04580) - 841178..841666 (+) 489 WP_126133318.1 ComE operon protein 2 -
  CYK59_RS04565 (CYK59_04585) comEC 841807..843921 (+) 2115 WP_241231961.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  CYK59_RS04570 (CYK59_04590) holA 843922..844950 (+) 1029 WP_076786858.1 DNA polymerase III subunit delta -
  CYK59_RS04575 (CYK59_04595) rpsT 845006..845260 (-) 255 WP_004271117.1 30S ribosomal protein S20 -
  CYK59_RS04580 (CYK59_04600) rpsO 845537..845806 (+) 270 WP_004271112.1 30S ribosomal protein S15 -

Sequence

Protein


Download         Length: 223 a.a.        Molecular weight: 24849.51 Da        Isoelectric Point: 9.9048

>NTDB_id=261900 CYK59_RS04555 WP_004270751.1 840448..841119(+) (comEA) [Latilactobacillus curvatus strain IRG2]
MWRDYWQRYRRFWWVPCLVIILLGGGVFWQHSRQQRFLSTNQQLMAQSTKRKVKAKPVKQKKSVSKGGFVDIKGAVAHPG
IYPICVAKTRLFEVLKQAGDVTEAADTSQLNLAQTLSDQLIIYVPTKGEAVQAEELVKAADEPQAATDNQAGASPISTDA
KINLNQADATQLQTLNGIGPKKAEQIIAYRDEQGQFKQIEDLQKVGGIGPKTFEQLRPQICVN

Nucleotide


Download         Length: 672 bp        

>NTDB_id=261900 CYK59_RS04555 WP_004270751.1 840448..841119(+) (comEA) [Latilactobacillus curvatus strain IRG2]
ATGTGGCGCGACTATTGGCAACGGTATCGTCGATTCTGGTGGGTACCTTGTTTGGTGATAATTTTACTTGGTGGTGGTGT
GTTTTGGCAGCATAGTCGGCAACAACGTTTTCTATCAACCAATCAACAATTGATGGCGCAATCAACTAAGCGAAAAGTGA
AGGCAAAACCCGTCAAACAGAAGAAAAGCGTCTCAAAAGGTGGGTTTGTCGATATCAAAGGCGCTGTGGCACATCCAGGG
ATTTATCCGATTTGTGTTGCAAAGACGCGTTTATTTGAGGTGCTGAAACAAGCTGGGGATGTGACCGAAGCAGCGGATAC
GAGTCAGTTGAACTTAGCGCAAACATTGAGTGACCAACTTATTATCTATGTGCCAACTAAGGGAGAAGCGGTGCAAGCCG
AAGAATTAGTGAAAGCGGCAGACGAGCCACAGGCTGCGACTGACAATCAAGCGGGAGCCAGTCCGATATCAACTGATGCT
AAAATCAACCTAAACCAAGCGGATGCGACACAATTGCAAACGCTAAATGGCATCGGTCCTAAAAAAGCTGAACAAATAAT
TGCATATCGGGATGAGCAAGGGCAGTTTAAACAAATCGAAGATCTTCAAAAAGTTGGGGGAATTGGGCCGAAAACGTTTG
AACAATTACGGCCACAAATTTGTGTTAATTAG

Domains

Predicted by InterproScan.

SLBB domain (SLBB)

(70-115)

Helix-hairpin-helix motif (HHH_3)

(160-221)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1B2A7J9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Latilactobacillus sakei subsp. sakei 23K

64.53

100

0.677
  comEA Lactococcus lactis subsp. cremoris KW2

36.283

100

0.368

Multiple sequence alignment