Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   CYK55_RS12720 Genome accession   NZ_CP025473
Coordinates   2614975..2616345 (-) Length   456 a.a.
NCBI ID   WP_019723034.1    Uniprot ID   A0A1L8V0P3
Organism   Enterococcus mundtii strain Pe161     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2609975..2621345
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CYK55_RS12705 (CYK55_12790) epsC 2611154..2611702 (-) 549 WP_034688627.1 serine O-acetyltransferase EpsC -
  CYK55_RS12710 (CYK55_12795) gltX 2612013..2613473 (-) 1461 WP_010734061.1 glutamate--tRNA ligase -
  CYK55_RS12715 (CYK55_12800) - 2613800..2614948 (-) 1149 WP_019723035.1 PIN/TRAM domain-containing protein -
  CYK55_RS12720 (CYK55_12805) radA 2614975..2616345 (-) 1371 WP_019723034.1 DNA repair protein RadA Machinery gene
  CYK55_RS12725 (CYK55_12810) - 2616411..2616938 (-) 528 WP_034688630.1 dUTP diphosphatase -
  CYK55_RS12730 (CYK55_12815) proB 2617154..2617957 (+) 804 WP_034688632.1 glutamate 5-kinase -
  CYK55_RS12735 (CYK55_12820) - 2617957..2619204 (+) 1248 WP_034688634.1 glutamate-5-semialdehyde dehydrogenase -
  CYK55_RS12740 (CYK55_12825) - 2619385..2619693 (-) 309 WP_034688677.1 hypothetical protein -
  CYK55_RS12745 (CYK55_12830) - 2619778..2620218 (-) 441 WP_019723030.1 DUF2798 domain-containing protein -
  CYK55_RS12750 (CYK55_12835) - 2620435..2620878 (+) 444 WP_164546252.1 hypothetical protein -

Sequence

Protein


Download         Length: 456 a.a.        Molecular weight: 49898.54 Da        Isoelectric Point: 7.0288

>NTDB_id=261856 CYK55_RS12720 WP_019723034.1 2614975..2616345(-) (radA) [Enterococcus mundtii strain Pe161]
MAKKAKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTDRRVRTSLTGEKAKPTKIAEVVPKKEPRVKTKLEELN
RVLGGGVVPGSMVLIGGDPGIGKSTLLLQVSQQLAAIGGKVLYVSGEESADQIKLRAERLGSIDTEFYLYAETDMNEISR
AIEQISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKIAKTNGIAIFIVGHVTKEGSIAGPRMLEHMVDTVLYFE
GEQHHSFRILRAVKNRFGSTNEIGIFEMHAHGLEEVANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFG
NAKRTTTGLDFNRVSLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECFIGEIGLTG
EIRRVNQIEQRVKEVQKLGFTKVYLPKNNLGGWNIPEGIEVVGVATLDETLRRVFR

Nucleotide


Download         Length: 1371 bp        

>NTDB_id=261856 CYK55_RS12720 WP_019723034.1 2614975..2616345(-) (radA) [Enterococcus mundtii strain Pe161]
ATGGCCAAAAAAGCAAAAGTTCAATTTGTTTGTCAGAATTGTGGCTATGTGTCGCCTAAATTTTTAGGACGTTGCCCAAA
TTGTGGCAAATGGAATACCATGGTTGAAGAAATCGAACAAGATACAACGGACCGCAGAGTACGAACGAGTTTGACTGGAG
AAAAAGCCAAGCCAACAAAAATCGCTGAGGTGGTTCCTAAGAAAGAACCACGAGTAAAGACGAAGTTAGAAGAATTGAAC
CGTGTTTTAGGTGGTGGTGTGGTTCCTGGCTCGATGGTTTTGATCGGTGGCGATCCTGGTATCGGAAAATCCACCTTACT
CTTGCAAGTATCACAGCAACTGGCTGCTATTGGTGGAAAAGTCCTTTACGTATCGGGAGAAGAGAGTGCGGACCAAATCA
AGTTGCGTGCAGAACGACTAGGTTCGATCGACACAGAGTTTTACTTGTATGCAGAGACAGATATGAATGAAATCAGTCGA
GCCATCGAACAAATTTCACCTGATTATGTCATCATCGATTCGATCCAAACAATGACTCAACCTGATATTACGAGTGTTGC
TGGGAGTGTTAGTCAAGTGCGAGAAACCACAGCTGAGCTATTGAAAATCGCAAAAACAAACGGTATTGCTATCTTTATAG
TAGGTCATGTCACGAAAGAAGGCTCGATTGCAGGTCCGCGCATGTTAGAGCACATGGTTGATACGGTCTTGTATTTTGAA
GGTGAGCAACATCATAGTTTTCGCATTTTGCGTGCCGTCAAAAATCGTTTTGGTTCCACCAATGAGATCGGTATTTTTGA
GATGCACGCCCATGGCCTTGAAGAAGTAGCCAATCCTTCACAGATTTTCTTAGAAGAACGATTGGATGGGGCGACAGGTT
CAGCGATCGTTGTCGCCATGGAGGGTACACGCCCAATCTTGGTTGAGATCCAAGCCTTAGTCACGCCCACGATGTTCGGA
AATGCCAAACGAACAACGACTGGTCTAGACTTTAATCGGGTGTCATTGATCATGGCAGTCTTAGAAAAACGGGCAGGATT
ATTGTTGCAAAATCAAGATGCTTACTTAAAAGCAGCTGGTGGGGTAAAATTAAATGAACCTGCGATCGATTTAGCGATTG
CAGTCAGTATTGCGTCTAGTTACAAAGAAAAAGGAACGCAACCAACGGAGTGTTTTATTGGAGAAATCGGTCTGACTGGT
GAAATCAGAAGAGTCAACCAAATTGAACAACGAGTCAAAGAAGTACAAAAATTAGGTTTTACAAAAGTATATTTACCTAA
AAATAATTTAGGTGGTTGGAATATTCCAGAGGGTATCGAAGTCGTAGGTGTTGCTACACTTGATGAAACTCTTAGACGAG
TATTTCGCTAA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(9-36)

Subunit ChlI of Mg-chelatase (ChlI)

(362-429)

AAA domain (AAA_25)

(69-221)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1L8V0P3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus pneumoniae Rx1

74.945

99.781

0.748
  radA Streptococcus pneumoniae D39

74.945

99.781

0.748
  radA Streptococcus pneumoniae R6

74.945

99.781

0.748
  radA Streptococcus pneumoniae TIGR4

74.945

99.781

0.748
  radA Streptococcus mitis NCTC 12261

74.945

99.781

0.748
  radA Streptococcus mitis SK321

74.945

99.781

0.748
  radA Bacillus subtilis subsp. subtilis str. 168

67.184

98.904

0.664

Multiple sequence alignment