Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   ABO_RS11485 Genome accession   NC_008260
Coordinates   2554215..2555279 (-) Length   354 a.a.
NCBI ID   WP_011589513.1    Uniprot ID   Q0VMB3
Organism   Alcanivorax borkumensis SK2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2549215..2560279
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABO_RS11460 (ABO_2232) aroK 2549705..2550241 (-) 537 WP_035459500.1 shikimate kinase AroK -
  ABO_RS11465 (ABO_2233) pilQ 2550238..2552409 (-) 2172 WP_011589509.1 type IV pilus secretin PilQ -
  ABO_RS11470 (ABO_2234) - 2552430..2552957 (-) 528 WP_011589510.1 pilus assembly protein PilP -
  ABO_RS11475 (ABO_2235) - 2552972..2553649 (-) 678 WP_011589511.1 type 4a pilus biogenesis protein PilO -
  ABO_RS11480 (ABO_2236) - 2553649..2554215 (-) 567 WP_011589512.1 PilN domain-containing protein -
  ABO_RS11485 (ABO_2237) pilM 2554215..2555279 (-) 1065 WP_011589513.1 pilus assembly protein PilM Machinery gene
  ABO_RS11490 (ABO_2238) - 2555479..2558043 (+) 2565 WP_011589514.1 penicillin-binding protein 1A -
  ABO_RS11495 (ABO_2239) - 2558154..2559428 (-) 1275 WP_011589515.1 malic enzyme-like NAD(P)-binding protein -
  ABO_RS11500 (ABO_2240) rpmE 2559537..2559755 (-) 219 WP_011589516.1 50S ribosomal protein L31 -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38263.40 Da        Isoelectric Point: 4.1901

>NTDB_id=26159 ABO_RS11485 WP_011589513.1 2554215..2555279(-) (pilM) [Alcanivorax borkumensis SK2]
MLPFLRKKAQTLLGIDISSTAVKLLELSKSGSRYKVESYGVEPLPANAVVEKNISDVEGVGDAIARLVSRTRPGVKAAAV
AVSGSAVITKLIEMDAELDEDALEMQLKSEADQYIPYPMDEVRMDFQVVGPVENNDERVEVLVAASRTENVEMRTDALEI
GGLQPRAVDVEAYAIERAFQLLLPGLPNAEELETVAVFDIGATMTTLNVFHQGRSVYTREQLFGGKQLTEEIQRRYGISM
EEAGLAKKTGELPDDYEGEVLQPFKDAIVQQVNSSLQFFYGSTQFNEVDYILLAGGSSSIADLGDLIQEKVGAGCSVANP
FSDMALANKVNASAIANDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=26159 ABO_RS11485 WP_011589513.1 2554215..2555279(-) (pilM) [Alcanivorax borkumensis SK2]
GTGCTGCCTTTCCTCAGAAAAAAGGCCCAGACACTACTGGGGATCGACATCAGCTCCACGGCGGTCAAGCTGTTGGAACT
GTCCAAATCAGGGAGTCGCTACAAGGTCGAGAGCTATGGGGTTGAGCCCCTGCCGGCCAATGCGGTTGTCGAGAAAAATA
TCAGCGACGTGGAAGGAGTGGGTGATGCTATTGCCCGCTTGGTAAGCCGTACGCGTCCCGGGGTAAAAGCGGCGGCGGTG
GCAGTGTCCGGTTCCGCTGTGATTACTAAGCTCATCGAAATGGATGCCGAGCTGGACGAGGATGCATTGGAAATGCAGCT
CAAGTCTGAGGCAGATCAGTACATCCCGTATCCCATGGATGAGGTCCGTATGGACTTTCAGGTTGTGGGGCCGGTTGAGA
ATAACGATGAGCGCGTGGAGGTGTTGGTTGCCGCCTCACGCACAGAGAACGTGGAGATGCGCACCGATGCGTTGGAAATC
GGTGGCCTACAACCGCGAGCGGTGGATGTGGAAGCGTACGCTATTGAGCGGGCTTTTCAGTTGCTTCTGCCCGGTTTGCC
GAATGCGGAGGAGCTAGAAACCGTGGCGGTATTCGATATCGGCGCCACCATGACCACGCTAAACGTATTTCATCAAGGGC
GTAGCGTTTATACCCGCGAGCAGCTTTTCGGGGGCAAGCAGCTCACTGAAGAAATTCAGCGTCGTTACGGTATTTCTATG
GAAGAGGCGGGGTTGGCCAAGAAAACCGGCGAGCTGCCCGATGATTACGAAGGCGAAGTGCTGCAGCCGTTCAAAGATGC
CATTGTCCAGCAGGTGAATAGTTCATTGCAGTTTTTCTATGGCTCCACGCAATTCAATGAAGTGGATTACATCTTGCTGG
CAGGGGGCTCTTCCTCTATCGCAGACTTGGGCGATCTGATTCAGGAAAAGGTGGGCGCGGGTTGTTCAGTGGCTAACCCT
TTCAGTGACATGGCGCTGGCGAACAAGGTGAATGCCTCTGCCATTGCCAATGATGCCCCGGCATTGATGATTGCCTGTGG
CCTGGCATTGAGGAGCTTTGACTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q0VMB3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

53.955

100

0.54
  comM Acinetobacter nosocomialis M2

53.955

100

0.54
  comM Acinetobacter baylyi ADP1

51.695

100

0.517
  pilM Legionella pneumophila strain ERS1305867

46.328

100

0.463

Multiple sequence alignment