Detailed information    

insolico Bioinformatically predicted

Overview

Name   radC   Type   Machinery gene
Locus tag   DSB73_RS22565 Genome accession   NZ_CP030767
Coordinates   4134729..4135397 (-) Length   222 a.a.
NCBI ID   WP_001297375.1    Uniprot ID   P65957
Organism   Escherichia coli strain 2017C-4109     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 4129729..4140397
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DSB73_RS22535 rfaQ 4129907..4130965 (-) 1059 WP_000360402.1 lipopolysaccharide core heptosyltransferase RfaQ -
  DSB73_RS22540 waaA 4131378..4132655 (+) 1278 WP_000891564.1 lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase -
  DSB73_RS22545 coaD 4132663..4133142 (+) 480 WP_001171866.1 pantetheine-phosphate adenylyltransferase -
  DSB73_RS22550 mutM 4133181..4133990 (-) 810 WP_001114533.1 bifunctional DNA-formamidopyrimidine glycosylase/DNA-(apurinic or apyrimidinic site) lyase -
  DSB73_RS22555 rpmG 4134088..4134255 (-) 168 WP_001051798.1 50S ribosomal protein L33 -
  DSB73_RS22560 rpmB 4134276..4134512 (-) 237 WP_000091955.1 50S ribosomal protein L28 -
  DSB73_RS22565 radC 4134729..4135397 (-) 669 WP_001297375.1 DNA repair protein RadC Machinery gene
  DSB73_RS22570 coaBC 4135569..4136789 (+) 1221 WP_000050139.1 bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC -
  DSB73_RS22575 dut 4136767..4137225 (+) 459 WP_000976070.1 dUTP diphosphatase -
  DSB73_RS22580 slmA 4137332..4137928 (+) 597 WP_000818601.1 nucleoid occlusion factor SlmA -
  DSB73_RS22585 pyrE 4137965..4138606 (-) 642 WP_000806161.1 orotate phosphoribosyltransferase -
  DSB73_RS22590 rph 4138672..4139388 (-) 717 WP_001247093.1 ribonuclease PH -
  DSB73_RS22595 yicC 4139515..4140378 (+) 864 WP_000621336.1 YicC/YloC family endoribonuclease -

Sequence

Protein


Download         Length: 222 a.a.        Molecular weight: 25258.35 Da        Isoelectric Point: 7.9748

>NTDB_id=260320 DSB73_RS22565 WP_001297375.1 4134729..4135397(-) (radC) [Escherichia coli strain 2017C-4109]
MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTLAKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQL
KGIAELARRYYNVRMREESPLLSPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAERGWI

Nucleotide


Download         Length: 669 bp        

>NTDB_id=260320 DSB73_RS22565 WP_001297375.1 4134729..4135397(-) (radC) [Escherichia coli strain 2017C-4109]
GTGAAAAACAATGCACAGCTGTTGATGCCGCGCGAAAAAATGCTGAAGTTTGGTATTAGCGCCTTAACTGATGTCGAGCT
GCTGGCGCTATTTCTGCGTACCGGAACGCGCGGTAAAGATGTATTAACCCTGGCAAAAGAGATGCTGGAGAATTTCGGCT
CTCTTTATGGCTTGTTAACCTCTGAATATGAACAATTTAGTGGTGTTCATGGAATTGGCGTAGCGAAATTTGCCCAGTTA
AAGGGGATTGCTGAACTGGCGCGACGTTACTACAACGTGCGGATGCGTGAAGAAAGCCCTTTACTCAGCCCGGAGATGAC
GCGGGAATTTTTACAAAGCCAGCTCACGGGTGAGGAGCGGGAGATCTTTATGGTGATCTTTCTCGACTCCCAACACCGGG
TTATAACGCATAGCCGTCTTTTTTCCGGCACGCTAAACCATGTTGAAGTCCATCCTCGGGAAATTATCCGCGAAGCGATA
AAAATAAACGCCTCGGCGCTGATCCTTGCACATAATCACCCTTCGGGTTGTGCTGAACCCAGTAAAGCGGATAAACTCAT
TACTGAACGGATAATAAAGAGTTGTCAGTTCATGGATTTACGCGTGCTCGACCATATCGTGATTGGGCGTGGAGAGTATG
TTTCTTTTGCCGAACGCGGCTGGATTTAA

Domains

Predicted by InterProScan.

RadC-like JAB domain (RadC)

(103-220)

UPF0758 N-terminal (UPF0758_N)

(9-77)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P65957

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radC Haemophilus influenzae Rd KW20

49.55

100

0.496

Multiple sequence alignment