Detailed information    

insolico Bioinformatically predicted

Overview

Name   sbcB   Type   Machinery gene
Locus tag   DSB73_RS13050 Genome accession   NZ_CP030767
Coordinates   2273997..2275421 (+) Length   474 a.a.
NCBI ID   WP_000132546.1    Uniprot ID   -
Organism   Escherichia coli strain 2017C-4109     
Function   promote homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2268997..2280421
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DSB73_RS13005 - 2269161..2269376 (+) 216 WP_000188870.1 hypothetical protein -
  DSB73_RS13010 - 2269475..2269696 (+) 222 WP_000763383.1 TraR/DksA family transcriptional regulator -
  DSB73_RS13015 - 2269693..2270640 (+) 948 WP_001289930.1 ead/Ea22-like family protein -
  DSB73_RS28920 - 2270642..2270818 (+) 177 WP_001356547.1 hypothetical protein -
  DSB73_RS13025 - 2271152..2271508 (+) 357 WP_000207903.1 hypothetical protein -
  DSB73_RS13030 - 2271505..2271855 (+) 351 WP_000610373.1 DUF551 domain-containing protein -
  DSB73_RS13035 - 2272043..2272387 (+) 345 WP_001281188.1 hypothetical protein -
  DSB73_RS13040 - 2272465..2272656 (+) 192 WP_000132739.1 AlpA family phage regulatory protein -
  DSB73_RS13045 - 2272637..2273815 (-) 1179 WP_001007946.1 site-specific integrase -
  DSB73_RS13050 sbcB 2273997..2275421 (+) 1425 WP_000132546.1 exodeoxyribonuclease I Machinery gene
  DSB73_RS13055 tsuB 2275464..2275691 (-) 228 WP_000985273.1 thiosulfate utilization sulfurtransferase TsuB/YeeD -
  DSB73_RS13060 tsuA 2275705..2276763 (-) 1059 WP_000492327.1 thiosulfate utilization transporter TsuA/YeeE -
  DSB73_RS13065 plaP 2276942..2278300 (-) 1359 WP_000019197.1 putrescine/proton symporter PlaP -
  DSB73_RS13070 yoeI 2278290..2278352 (-) 63 WP_010723108.1 membrane protein YoeI -
  DSB73_RS13075 yeeY 2278567..2279496 (-) 930 WP_000803334.1 LysR substrate-binding domain-containing protein -
  DSB73_RS13080 yeeZ 2279542..2280366 (-) 825 WP_000754737.1 SDR family oxidoreductase -

Sequence

Protein


Download         Length: 474 a.a.        Molecular weight: 54397.63 Da        Isoelectric Point: 5.2440

>NTDB_id=260291 DSB73_RS13050 WP_000132546.1 2273997..2275421(+) (sbcB) [Escherichia coli strain 2017C-4109]
MTDTDKQPTFLFHDYETFGTHPALDRPAQFAAIRTDDEFNVIGEPEVFYCKPADDYLPQPGAVLITGITPQEARAKGENE
AAFAARIHSLFTVPKTCILGYNNVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDGL
PSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLMALIDVPQMKPLVHVSGMFGAWRG
NTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDSDTLRERLYTAKTDLGDNAAVPVKLVHINKCPVLAQANTLRPEDAD
RLGINRQHCLDNLKILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLPALDITFVDK
RIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYAEEIQMLAQQYADDKEKVALLKALWQYAEEIV

Nucleotide


Download         Length: 1425 bp        

>NTDB_id=260291 DSB73_RS13050 WP_000132546.1 2273997..2275421(+) (sbcB) [Escherichia coli strain 2017C-4109]
ATGACAGACACAGATAAGCAACCTACCTTCCTCTTTCACGATTACGAAACCTTTGGCACGCACCCCGCGTTAGATCGCCC
TGCACAGTTCGCAGCCATTCGCACCGATGACGAATTCAATGTCATCGGCGAACCCGAAGTCTTTTACTGCAAGCCCGCGG
ATGACTATTTACCCCAGCCTGGAGCAGTATTAATTACCGGTATTACCCCGCAGGAAGCTCGGGCGAAAGGAGAAAACGAA
GCCGCATTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGA
CGACGAAGTCACACGCAACGTTTTTTATCGTAATTTCTACGATCCTTACGCCTGGAGCTGGCAGCATGATAACTCGCGCT
GGGATTTACTGGATGTTATGCGTGCCTGCTATGCCCTGCGCCCGGAAGGAATAAACTGGCCTGAAAATGATGACGGTCTA
CCGAGCTTTCGCCTTGAGCATTTAACCAAAGCGAATGGTATTGAACATAGCAACGCCCACGATGCGATGGCTGATGTGTA
CGCCACTATTGCGATGGCGAAACTGGTAAAAACGCGTCAACCACGCCTGTTTGATTATCTCTTTACCCATCGTAATAAAC
ACAAACTGATGGCGTTGATTGATGTTCCGCAGATGAAACCCCTGGTTCACGTTTCCGGAATGTTTGGGGCATGGCGCGGC
AATACCAGCTGGGTGGCACCGCTGGCGTGGCATCCTGAAAATCGCAATGCCGTAATTATGGTGGATTTGGCAGGAGATAT
TTCGCCATTACTGGAACTGGATAGCGACACATTGCGCGAGCGTTTATATACCGCAAAAACCGATCTTGGCGATAACGCCG
CCGTTCCGGTTAAACTGGTACATATCAATAAATGTCCGGTGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGAC
CGACTGGGAATTAATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGTGAAAAAGTGGT
GGCGATATTCGCGGAAGCCGAACCGTTTACGCCTTCAGATAACGTGGATGCACAGCTTTATAATGGCTTTTTCAGTGACG
CCGATCGTGCAGCAATGAAAATTGTGCTTGAAACCGAGCCGCGTAATTTACCGGCACTGGATATCACTTTTGTTGATAAA
CGGATTGAAAAGCTGTTGTTCAATTATCGGGCACGCAACTTCCCGGGGACGCTGGATTATGCCGAGCAGCAACGCTGGCT
GGAGCACCGCCGCCAGGTCTTCACGCCAGAGTTTTTACAAGGCTATGCAGAAGAGATTCAGATGCTGGCGCAGCAGTATG
CCGACGATAAAGAAAAAGTGGCGCTGTTAAAAGCGCTTTGGCAGTACGCGGAAGAGATTGTCTAA

Domains

Predicted by InterProScan.

Exonuclease (RNase_T)

(12-187)

Exonuclease C-terminal (ExoI_SH3)

(211-471)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  sbcB Vibrio cholerae O1 biovar El Tor strain E7946

58.936

99.156

0.584