Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA/sms   Type   Machinery gene
Locus tag   DRF63_RS04680 Genome accession   NZ_CP030753
Coordinates   1028174..1029553 (+) Length   459 a.a.
NCBI ID   WP_005597398.1    Uniprot ID   A0ABN5MKE6
Organism   Actinobacillus pleuropneumoniae strain S4074     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1023174..1034553
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DRF63_RS04650 (DRF63_04645) yacG 1023453..1023641 (-) 189 WP_005597388.1 DNA gyrase inhibitor YacG -
  DRF63_RS04655 (DRF63_04650) coaE 1023623..1024261 (-) 639 WP_005597390.1 dephospho-CoA kinase -
  DRF63_RS04660 (DRF63_04655) - 1024283..1024921 (-) 639 WP_005597392.1 prepilin peptidase -
  DRF63_RS04665 (DRF63_04660) pilC 1024948..1026150 (-) 1203 WP_005597394.1 type II secretion system F family protein Machinery gene
  DRF63_RS04670 (DRF63_04665) pilB 1026134..1027528 (-) 1395 WP_005597396.1 GspE/PulE family protein Machinery gene
  DRF63_RS04675 (DRF63_04670) pilA 1027546..1027992 (-) 447 WP_005597397.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  DRF63_RS04680 (DRF63_04675) radA/sms 1028174..1029553 (+) 1380 WP_005597398.1 DNA repair protein RadA Machinery gene
  DRF63_RS04685 (DRF63_04680) - 1029597..1029830 (-) 234 WP_005597399.1 YgjV family protein -
  DRF63_RS04690 (DRF63_04685) folA 1029957..1030445 (-) 489 WP_005597400.1 type 3 dihydrofolate reductase -
  DRF63_RS04695 (DRF63_04690) proB 1030532..1031623 (+) 1092 WP_005597401.1 glutamate 5-kinase -
  DRF63_RS04700 (DRF63_04695) - 1031636..1032412 (+) 777 WP_005597402.1 TSUP family transporter -
  DRF63_RS04705 (DRF63_04700) nth 1032413..1033045 (+) 633 WP_005597404.1 endonuclease III -

Sequence

Protein


Download         Length: 459 a.a.        Molecular weight: 49691.24 Da        Isoelectric Point: 7.9094

>NTDB_id=260239 DRF63_RS04680 WP_005597398.1 1028174..1029553(+) (radA/sms) [Actinobacillus pleuropneumoniae strain S4074]
MAKAPKTAYVCNDCGAEYSRWMGQCKECKSWNTVSEIRLISSKEATKSDRFSGYAGETSGKVQTLSEISLQEVPRFSSGF
NELDRVLGGGVVPGSAILIGGHPGAGKSTLLLQVMCGLSKDLPTLYVTGEESLQQVAMRANRLGLPTDNLKMLSETSVEH
ICNIADQEKPKIMVIDSIQVMHLADIQSSPGSVSQVRECASFLTRYAKNRQVAIIMVGHVTKDGTLAGPKVLEHCIDASI
LLEGESDSRFRTLRSQKNRFGAVNELGVFAMTEQGLREVKNPSAIFLSRSDEQTPGSSVMVLWEGTRPLLVEIQALVDHS
MLANPRRVAVGLDHNRLSLLLAVLHRHGGLQMSDQDVFVNVVGGVKVTETSADLALILALISSFRNRALPQDLVVFGEVG
LAGEIRPVPSGQERISEAAKHGFKRAIIPHGNAPKKPIKGMEVFTVKKLSDALDILGNL

Nucleotide


Download         Length: 1380 bp        

>NTDB_id=260239 DRF63_RS04680 WP_005597398.1 1028174..1029553(+) (radA/sms) [Actinobacillus pleuropneumoniae strain S4074]
ATGGCTAAAGCACCAAAAACAGCTTATGTATGTAATGATTGCGGCGCGGAATATTCTCGTTGGATGGGGCAATGCAAAGA
ATGTAAGTCTTGGAATACCGTGAGTGAAATCAGACTTATCTCGAGTAAAGAAGCAACAAAAAGCGACCGCTTCAGCGGAT
ATGCCGGCGAAACCTCCGGTAAAGTTCAAACTTTATCCGAAATCAGCTTACAAGAAGTGCCTCGTTTTAGCAGCGGCTTT
AATGAATTGGATCGCGTACTGGGCGGCGGTGTCGTGCCGGGTTCGGCAATTTTAATCGGCGGCCATCCCGGTGCCGGTAA
ATCCACTCTGCTTTTACAAGTAATGTGCGGTTTGTCTAAAGATTTGCCGACCCTTTACGTGACCGGTGAAGAATCCCTAC
AACAAGTGGCGATGCGAGCGAATCGTTTAGGCTTACCGACCGACAATCTCAAGATGCTTTCAGAAACTTCGGTCGAGCAT
ATTTGTAATATCGCCGACCAAGAAAAGCCGAAAATTATGGTAATCGACTCAATTCAGGTCATGCACCTTGCCGATATTCA
ATCTTCTCCCGGTAGCGTTTCGCAAGTGCGGGAATGCGCTTCGTTTCTGACTCGCTATGCAAAAAACCGCCAAGTAGCGA
TTATTATGGTCGGACACGTCACCAAAGACGGGACACTTGCCGGCCCGAAAGTGTTGGAACACTGTATTGACGCCTCAATT
TTACTGGAAGGCGAATCGGACTCTCGATTCCGTACCTTACGCAGCCAGAAAAACCGTTTCGGTGCGGTCAATGAATTGGG
CGTATTTGCCATGACCGAACAAGGCTTACGAGAAGTAAAAAACCCTTCCGCGATTTTCCTCAGCCGTAGCGATGAACAAA
CGCCGGGCAGTTCCGTGATGGTGCTTTGGGAAGGAACCCGTCCGTTATTAGTCGAAATTCAAGCGTTGGTTGATCATTCG
ATGTTGGCTAATCCTCGCCGTGTCGCCGTAGGTTTAGATCACAACCGTTTATCGCTCTTACTTGCGGTGTTACATCGCCA
CGGCGGTTTGCAAATGTCGGATCAAGATGTGTTCGTCAATGTGGTCGGCGGGGTAAAAGTGACCGAGACCAGTGCCGATC
TCGCCCTAATTTTGGCGTTAATTTCCAGTTTTAGAAATCGTGCGTTACCGCAAGATTTGGTCGTATTCGGCGAGGTGGGA
TTAGCCGGAGAAATTCGTCCGGTACCGAGCGGTCAAGAACGTATCAGCGAAGCGGCGAAGCACGGTTTTAAACGTGCGAT
TATTCCTCACGGCAACGCACCTAAAAAACCGATTAAAGGTATGGAAGTCTTTACCGTTAAGAAACTAAGCGACGCACTAG
ATATTCTCGGCAATCTCTAA

Domains

Predicted by InterProScan.

Subunit ChlI of Mg-chelatase (ChlI)

(347-432)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(9-36)

AAA domain (AAA_25)

(83-224)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA/sms Bacillus subtilis subsp. subtilis str. 168

48.14

99.564

0.479
  radA Streptococcus mitis NCTC 12261

44.273

98.911

0.438
  radA Streptococcus mitis SK321

44.053

98.911

0.436
  radA Streptococcus pneumoniae Rx1

45.349

93.682

0.425
  radA Streptococcus pneumoniae D39

45.349

93.682

0.425
  radA Streptococcus pneumoniae R6

45.349

93.682

0.425
  radA Streptococcus pneumoniae TIGR4

45.349

93.682

0.425