Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   CN246_RS01675 Genome accession   NZ_CP025288
Coordinates   352875..353813 (+) Length   312 a.a.
NCBI ID   WP_013484288.1    Uniprot ID   E6U7X5
Organism   Ethanoligenens harbinense strain X-29     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 347875..358813
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CN246_RS01665 (CN246_01650) - 348438..349148 (+) 711 WP_013484286.1 NAD(P)H-dependent oxidoreductase -
  CN246_RS01670 (CN246_01655) smc 349188..352769 (+) 3582 WP_013484287.1 chromosome segregation protein SMC -
  CN246_RS01675 (CN246_01660) pilA 352875..353813 (+) 939 WP_013484288.1 signal recognition particle-docking protein FtsY Machinery gene
  CN246_RS01680 (CN246_01665) rdgB 353840..354430 (+) 591 WP_013484289.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -
  CN246_RS14945 - 354450..354818 (+) 369 WP_013484290.1 carboxylesterase family protein -
  CN246_RS01690 (CN246_01675) - 354851..355138 (+) 288 WP_013484291.1 YhbY family RNA-binding protein -
  CN246_RS01695 (CN246_01680) nadD 355152..355778 (+) 627 WP_013484292.1 nicotinate-nucleotide adenylyltransferase -
  CN246_RS01700 (CN246_01685) yqeK 355796..356362 (+) 567 WP_041687023.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  CN246_RS01705 (CN246_01690) rsfS 356530..356934 (+) 405 WP_013484294.1 ribosome silencing factor -

Sequence

Protein


Download         Length: 312 a.a.        Molecular weight: 32871.64 Da        Isoelectric Point: 5.0152

>NTDB_id=259871 CN246_RS01675 WP_013484288.1 352875..353813(+) (pilA) [Ethanoligenens harbinense strain X-29]
MGVFDKIRAGLAKTRASVAGRMGQVFHGRGKVDEELFEELEDVLVSSDVGVATSTYLIEKLRGRVKKEHIVDTDGVREAL
KAVMTGELEGDVSLLLAPAPAVLLVIGVNGVGKTTTIGKLAAQLKAEGKAVLLGAADTFRAAAIDQLEIWAERAGVDIVK
QKEGADPAAVVFDAVQAGKARGADVVICDTAGRLHNKKSLMDELSKISRVIDRELPGAARENLLVLDATTGQNAVNQAKQ
FKEAAGLTGIILTKLDGTARGGVVFAIRHELGVPVKYIGVGEQIDDLQPFDPRAFVDALFQGADEEAAGQDG

Nucleotide


Download         Length: 939 bp        

>NTDB_id=259871 CN246_RS01675 WP_013484288.1 352875..353813(+) (pilA) [Ethanoligenens harbinense strain X-29]
ATGGGCGTTTTTGATAAGATCCGCGCGGGGCTGGCCAAGACCCGCGCGTCGGTCGCGGGGCGGATGGGGCAGGTGTTCCA
CGGGCGCGGCAAGGTGGACGAGGAACTGTTCGAAGAACTGGAGGACGTGCTGGTCTCCTCCGACGTGGGTGTGGCAACTT
CCACCTATCTCATCGAAAAGCTGCGCGGCCGGGTGAAAAAAGAACACATCGTCGATACCGACGGCGTGCGCGAGGCGCTC
AAGGCCGTGATGACGGGGGAACTGGAAGGCGATGTGTCGCTCCTGTTGGCTCCCGCACCGGCGGTGCTGCTGGTCATCGG
GGTCAACGGCGTGGGCAAGACCACCACCATCGGCAAGCTGGCCGCCCAGCTTAAAGCCGAGGGAAAAGCGGTGCTGCTGG
GCGCGGCGGATACGTTCCGCGCCGCCGCCATCGACCAGTTGGAGATCTGGGCGGAGCGCGCCGGGGTGGACATCGTCAAG
CAGAAAGAGGGCGCCGATCCCGCCGCCGTAGTGTTCGACGCGGTGCAGGCCGGCAAGGCGCGCGGTGCGGACGTGGTCAT
CTGCGACACGGCCGGCCGCCTGCACAATAAAAAGAGCCTGATGGACGAACTGTCGAAGATTTCCCGCGTGATCGACCGGG
AACTGCCGGGCGCGGCGCGCGAAAACCTGCTTGTGCTCGACGCGACCACCGGGCAGAACGCCGTCAACCAGGCCAAGCAG
TTCAAAGAGGCCGCCGGGCTCACCGGCATCATCCTCACCAAGCTCGACGGCACCGCGCGCGGCGGCGTGGTCTTCGCCAT
CCGCCATGAGCTGGGTGTGCCGGTGAAATACATCGGCGTGGGCGAGCAGATCGACGATTTGCAGCCGTTCGATCCCCGCG
CGTTCGTGGACGCATTGTTCCAGGGTGCGGACGAGGAAGCCGCCGGGCAGGACGGTTGA

Domains

Predicted by InterproScan.

SRP54-type protein, helical bundle domain (SRP54_N)

(20-82)

SRP54-type protein, GTPase domain (SRP54)

(101-300)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB E6U7X5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

50.825

97.115

0.494

Multiple sequence alignment