Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   CXG95_RS18270 Genome accession   NZ_CP025226
Coordinates   3515332..3515778 (+) Length   148 a.a.
NCBI ID   WP_003178810.1    Uniprot ID   A0A8B5YEZ5
Organism   Bacillus licheniformis strain PB3     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 3510332..3520778
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CXG95_RS18255 (CXG95_18250) - 3511493..3512233 (+) 741 WP_003178817.1 LXG domain-containing protein -
  CXG95_RS18260 (CXG95_18255) - 3512424..3514033 (+) 1610 Protein_3570 methyl-accepting chemotaxis protein -
  CXG95_RS18265 (CXG95_18260) - 3514053..3514856 (-) 804 WP_017473997.1 protein kinase -
  CXG95_RS21925 - 3514862..3515032 (-) 171 WP_011197533.1 hypothetical protein -
  CXG95_RS18270 (CXG95_18265) nucA/comI 3515332..3515778 (+) 447 WP_003178810.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  CXG95_RS18275 (CXG95_18270) nin/comJ 3515796..3516194 (+) 399 WP_003178808.1 competence protein ComJ Regulator
  CXG95_RS18280 (CXG95_18275) - 3516233..3517669 (-) 1437 WP_003178806.1 family 1 glycosylhydrolase -
  CXG95_RS18285 (CXG95_18280) - 3517739..3518464 (-) 726 WP_003178804.1 AAA family ATPase -
  CXG95_RS18290 (CXG95_18285) - 3518561..3519112 (-) 552 WP_003178803.1 NAD(P)H-dependent oxidoreductase -
  CXG95_RS18295 (CXG95_18290) - 3519296..3520171 (+) 876 WP_003178801.1 transporter substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 148 a.a.        Molecular weight: 16224.12 Da        Isoelectric Point: 4.9954

>NTDB_id=259216 CXG95_RS18270 WP_003178810.1 3515332..3515778(+) (nucA/comI) [Bacillus licheniformis strain PB3]
MDKIKSLLITLLAVAVVIIGIVSGDFFTSEHQPSGRQNGSNYDEVLIFPSDRYPETGAHIRKAIKKGHSEICTIDRDGAD
ERRKDSLKDVPSKSGYDRDEWPMAMCEEGGTGASVEYISPSDNRGAGSWVGNQVSDYPDGTKVLFKIN

Nucleotide


Download         Length: 447 bp        

>NTDB_id=259216 CXG95_RS18270 WP_003178810.1 3515332..3515778(+) (nucA/comI) [Bacillus licheniformis strain PB3]
ATGGACAAGATTAAATCTTTGCTGATCACCTTGCTCGCCGTCGCCGTCGTGATCATCGGTATTGTATCTGGCGACTTTTT
CACTTCAGAACACCAGCCTTCGGGCCGGCAAAACGGCTCAAACTACGATGAAGTGCTGATTTTTCCTTCGGACCGCTATC
CTGAAACAGGCGCACACATTCGAAAGGCTATCAAAAAAGGGCACTCCGAAATCTGTACGATCGACAGGGACGGAGCCGAC
GAACGCCGAAAGGATTCATTGAAAGATGTTCCTTCCAAATCAGGCTATGACCGCGACGAGTGGCCGATGGCCATGTGCGA
AGAAGGCGGAACAGGTGCTTCTGTCGAGTATATCAGCCCGTCTGATAACCGTGGAGCAGGATCTTGGGTGGGCAATCAGG
TAAGCGATTATCCGGACGGGACGAAAGTACTTTTCAAGATTAATTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(53-143)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A8B5YEZ5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

64.626

99.324

0.642

Multiple sequence alignment