Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   CXG95_RS06505 Genome accession   NZ_CP025226
Coordinates   1251997..1252626 (+) Length   209 a.a.
NCBI ID   WP_003183693.1    Uniprot ID   Q8VQ77
Organism   Bacillus licheniformis strain PB3     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1246997..1257626
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CXG95_RS06470 (CXG95_06470) aroE 1247639..1248475 (+) 837 WP_003183706.1 shikimate dehydrogenase -
  CXG95_RS06475 (CXG95_06475) yhbY 1248469..1248759 (+) 291 WP_003183704.1 ribosome assembly RNA-binding protein YhbY -
  CXG95_RS06480 (CXG95_06480) - 1248778..1249347 (+) 570 WP_003183702.1 nicotinate-nucleotide adenylyltransferase -
  CXG95_RS06485 (CXG95_06485) yqeK 1249337..1249900 (+) 564 WP_003183701.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  CXG95_RS06490 (CXG95_06490) rsfS 1249914..1250270 (+) 357 WP_003183699.1 ribosome silencing factor -
  CXG95_RS06495 (CXG95_06495) - 1250267..1251022 (+) 756 WP_003183697.1 class I SAM-dependent methyltransferase -
  CXG95_RS06500 (CXG95_06500) comER 1251092..1251913 (-) 822 WP_003183696.1 late competence protein ComER -
  CXG95_RS06505 (CXG95_06505) comEA 1251997..1252626 (+) 630 WP_003183693.1 helix-hairpin-helix domain-containing protein Machinery gene
  CXG95_RS06510 (CXG95_06510) - 1252695..1253264 (+) 570 WP_003183690.1 ComE operon protein 2 -
  CXG95_RS06515 (CXG95_06515) - 1253268..1255655 (+) 2388 WP_061565979.1 DNA internalization-related competence protein ComEC/Rec2 -
  CXG95_RS06520 (CXG95_06520) - 1255680..1255811 (-) 132 WP_003183686.1 YqzM family protein -
  CXG95_RS06525 (CXG95_06525) holA 1256041..1257084 (+) 1044 WP_009327866.1 DNA polymerase III subunit delta -
  CXG95_RS06530 (CXG95_06530) rpsT 1257146..1257412 (-) 267 WP_003183682.1 30S ribosomal protein S20 -

Sequence

Protein


Download         Length: 209 a.a.        Molecular weight: 22468.42 Da        Isoelectric Point: 5.1890

>NTDB_id=259176 CXG95_RS06505 WP_003183693.1 1251997..1252626(+) (comEA) [Bacillus licheniformis strain PB3]
MTDWLKQYKWHAAGGVALVLIISAAFMLLSGKRETSSGLSIPEEASAQTYDKKEEVKREKSAGKEAVIIDLKGAVKNPGV
YQMKEGDRVHDVLKKAGGTEKKADQKQINLAAVLQDGMVVYIPFEGEEAADSFSKAGSRADGASVDIVNINTASSEELQA
IPGIGPSKAEAVIEYREENGPFHTVEDITNVSGIGEKSFERIKSAITVK

Nucleotide


Download         Length: 630 bp        

>NTDB_id=259176 CXG95_RS06505 WP_003183693.1 1251997..1252626(+) (comEA) [Bacillus licheniformis strain PB3]
TTGACAGATTGGCTAAAACAATATAAATGGCATGCTGCGGGAGGCGTTGCACTCGTCTTGATCATCAGCGCTGCCTTCAT
GCTGTTAAGCGGAAAGCGTGAAACTTCATCCGGCCTCTCCATTCCCGAGGAGGCTTCTGCACAGACGTATGACAAGAAGG
AGGAGGTGAAACGGGAAAAAAGCGCGGGAAAAGAGGCGGTTATCATCGATTTGAAAGGCGCTGTGAAAAATCCGGGCGTC
TATCAAATGAAAGAGGGAGACAGGGTCCACGATGTATTGAAAAAAGCAGGCGGCACCGAGAAAAAAGCGGATCAAAAGCA
AATTAACCTTGCAGCCGTTTTGCAGGACGGTATGGTGGTTTACATTCCATTTGAGGGGGAAGAGGCTGCTGATTCTTTCT
CGAAAGCGGGTTCAAGGGCAGATGGCGCTTCCGTCGATATCGTCAATATCAATACGGCTTCCTCTGAGGAGCTTCAGGCG
ATTCCCGGCATCGGCCCTTCAAAAGCGGAAGCGGTTATCGAATACCGCGAGGAGAACGGACCGTTTCACACGGTAGAAGA
CATAACAAACGTTTCGGGAATTGGAGAAAAGTCTTTTGAAAGAATAAAATCTGCAATCACGGTAAAGTAA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(147-207)

SLBB domain (SLBB)

(69-123)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q8VQ77

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

51.402

100

0.526

Multiple sequence alignment