Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   CXB72_RS01850 Genome accession   NZ_CP025200
Coordinates   346415..347791 (+) Length   458 a.a.
NCBI ID   WP_125977856.1    Uniprot ID   -
Organism   Lactobacillus acidophilus strain YT1     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 341415..352791
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CXB72_RS10635 (CXB72_01825) - 342064..342246 (+) 183 WP_003549074.1 hypothetical protein -
  CXB72_RS01825 (CXB72_01830) - 342248..342925 (+) 678 WP_021721658.1 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase -
  CXB72_RS01830 (CXB72_01835) - 343030..343965 (+) 936 WP_003549076.1 AAA family ATPase -
  CXB72_RS01835 (CXB72_01840) pepC 344016..345365 (-) 1350 WP_003549077.1 aminopeptidase C -
  CXB72_RS01840 (CXB72_01845) - 345436..345732 (-) 297 WP_003549078.1 hypothetical protein -
  CXB72_RS01845 (CXB72_01850) - 345864..346415 (+) 552 WP_011254121.1 dUTP diphosphatase -
  CXB72_RS01850 (CXB72_01855) radA 346415..347791 (+) 1377 WP_125977856.1 DNA repair protein RadA Machinery gene
  CXB72_RS01855 (CXB72_01860) gltX 347869..349368 (+) 1500 WP_003549081.1 glutamate--tRNA ligase -
  CXB72_RS01860 (CXB72_01865) cysS 349466..350899 (+) 1434 WP_125977858.1 cysteine--tRNA ligase -
  CXB72_RS01865 (CXB72_01870) - 350892..351335 (+) 444 WP_003549083.1 Mini-ribonuclease 3 -
  CXB72_RS01870 (CXB72_01875) rlmB 351322..352074 (+) 753 WP_125977860.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  CXB72_RS01875 (CXB72_01880) - 352214..352750 (+) 537 WP_125977862.1 sigma-70 family RNA polymerase sigma factor -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 50197.73 Da        Isoelectric Point: 6.9041

>NTDB_id=258743 CXB72_RS01850 WP_125977856.1 346415..347791(+) (radA) [Lactobacillus acidophilus strain YT1]
MARVKTQYKCRSCGYISASYLGRCPNCGAWNQFEKETEEVQKRSTKATASRLIQKTGVNEPVKLDKIKAEKEERISTKSE
ELNRVLGGGIVPGSLVLIGGDPGIGKSTLMLQIMSDLAEKHKVLYVSGEESANQIKLRDDRLGIGQSNMLLYPESDMHDI
RGQINDVKPDFVVIDSIQTMNEPSLDSMTGSASQVREVTSELMKIAKMDAITVFVIGHVTKEGAIAGPKIMEHMVDTVLY
FEGDEHHSYRILHSVKNRFGAANEIGMFEMVNDGLKEVTNPSAIFLDQRLPNSTGSAVVVSLEGTRPLLAEIQALVTPTA
FGYAKRTTSGIDYNRAALLLAVLEKRGNLMLQNQDVYLTATGGIKLNEPAIDLAIAMSVASSYTDKEISPTDCFVGEVGL
TGEIRRVDKIEARVKEAAKVGFKRIFIPKHNMYQGLTNNGIEVIPVSSIPQALKLVFN

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=258743 CXB72_RS01850 WP_125977856.1 346415..347791(+) (radA) [Lactobacillus acidophilus strain YT1]
ATGGCAAGGGTAAAAACTCAATATAAATGTCGCTCCTGTGGTTACATTTCTGCTAGTTATTTAGGTAGATGTCCTAACTG
TGGAGCATGGAATCAATTTGAAAAAGAAACTGAAGAAGTACAAAAACGTTCTACCAAAGCTACAGCTAGCCGTTTAATCC
AAAAAACTGGCGTAAATGAACCAGTAAAGCTAGATAAAATTAAAGCGGAAAAAGAAGAGAGAATTTCTACTAAATCAGAA
GAATTAAATCGTGTTTTAGGTGGCGGAATTGTGCCTGGATCGCTTGTATTAATCGGTGGAGATCCTGGAATTGGTAAATC
AACTTTAATGTTGCAAATTATGAGTGATTTGGCAGAAAAGCATAAAGTATTATACGTTTCCGGAGAAGAATCAGCTAATC
AAATTAAGCTAAGAGATGATCGGCTAGGAATCGGGCAGAGTAACATGCTTTTATATCCTGAAAGTGATATGCATGATATT
CGTGGACAAATTAATGATGTTAAACCTGATTTTGTAGTTATTGATTCAATTCAAACAATGAATGAACCTAGCTTAGATTC
TATGACAGGTTCTGCCTCACAGGTTCGCGAAGTAACTAGTGAATTGATGAAAATCGCAAAAATGGATGCAATTACTGTTT
TTGTAATTGGACATGTAACTAAAGAGGGTGCAATTGCAGGACCTAAAATTATGGAACATATGGTGGATACTGTTCTATAT
TTTGAAGGGGATGAGCACCATTCTTATAGAATTTTACATTCTGTGAAAAACCGTTTTGGTGCCGCTAATGAAATTGGCAT
GTTTGAAATGGTGAATGACGGGTTAAAAGAAGTAACTAATCCGTCAGCTATTTTTCTTGATCAAAGATTACCAAATTCAA
CTGGTTCAGCAGTTGTTGTTTCTTTGGAAGGAACACGACCGCTTTTGGCAGAAATTCAGGCTTTGGTTACGCCAACGGCA
TTTGGTTATGCAAAAAGAACAACGTCAGGGATTGATTATAATAGGGCTGCTCTTTTACTGGCAGTACTTGAAAAGCGTGG
TAATTTGATGTTGCAAAATCAAGATGTATATTTGACTGCTACTGGTGGGATTAAGTTGAATGAGCCTGCGATTGATTTGG
CGATTGCAATGTCTGTTGCATCTAGTTATACAGACAAAGAAATTTCACCAACTGATTGTTTTGTAGGTGAAGTAGGTTTA
ACGGGAGAAATCCGTAGAGTAGATAAAATTGAAGCTAGAGTAAAAGAAGCTGCTAAAGTTGGTTTTAAGCGAATTTTTAT
TCCTAAACACAATATGTATCAAGGATTAACTAATAACGGAATAGAAGTTATTCCAGTTTCAAGCATTCCGCAAGCGTTAA
AATTAGTTTTTAATTAG

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(363-431)

AAA domain (AAA_25)

(66-223)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-34)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus pneumoniae Rx1

57.112

99.782

0.57
  radA Streptococcus pneumoniae D39

57.112

99.782

0.57
  radA Streptococcus pneumoniae R6

57.112

99.782

0.57
  radA Streptococcus pneumoniae TIGR4

57.112

99.782

0.57
  radA Streptococcus mitis SK321

57.112

99.782

0.57
  radA Streptococcus mitis NCTC 12261

57.112

99.782

0.57
  radA Bacillus subtilis subsp. subtilis str. 168

55.604

99.345

0.552

Multiple sequence alignment