Detailed information    

insolico Bioinformatically predicted

Overview

Name   helD   Type   Machinery gene
Locus tag   DOU34_RS14715 Genome accession   NZ_CP030097
Coordinates   2980135..2982450 (-) Length   771 a.a.
NCBI ID   WP_061047083.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain SH-B74     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2975135..2987450
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DOU34_RS14705 cysI 2976409..2978124 (-) 1716 WP_003151735.1 assimilatory sulfite reductase (NADPH) hemoprotein subunit -
  DOU34_RS14710 - 2978152..2979960 (-) 1809 WP_042635485.1 assimilatory sulfite reductase (NADPH) flavoprotein subunit -
  DOU34_RS14715 helD 2980135..2982450 (-) 2316 WP_061047083.1 RNA polymerase recycling motor HelD Machinery gene
  DOU34_RS14720 - 2982622..2983230 (-) 609 WP_007410017.1 trimeric intracellular cation channel family protein -
  DOU34_RS14725 - 2983453..2984253 (+) 801 WP_061047082.1 zinc-dependent metalloprotease -
  DOU34_RS14730 - 2984287..2984706 (-) 420 WP_012118443.1 disulfide oxidoreductase -
  DOU34_RS14735 - 2984696..2985367 (-) 672 WP_012118444.1 DsbA family protein -

Sequence

Protein


Download         Length: 771 a.a.        Molecular weight: 88154.28 Da        Isoelectric Point: 6.1590

>NTDB_id=258600 DOU34_RS14715 WP_061047083.1 2980135..2982450(-) (helD) [Bacillus amyloliquefaciens strain SH-B74]
MAKRDKEWKEEQERINFVMKELEQKQTHLEASSGGLKQDIVGLRKTFWDDVTVNMDDAHEAAETFSSIKQQAEMLSERER
THRLQYRQLQKIQKLKTSPYFGRVDFTEKGESSSERIYIGLTSLMDENNEQFLIYDWRAPISSLYYQYSPGKARYEVPEE
TIEGDITLKRQFIIKNGTLKAMFNTDMTIGDEMLQEVLSGHSGQHMKNIVSTIQKEQNEIIRDEKSKYLIVQGAAGSGKT
SAALQRAAYLLYRGRGVLDARQIVLFSPNILFNSYVSAVLPELGEENMEQATFQEYILRRLGRKFDCESPYEQLEYCLTE
QNQDVLFVRLAAIAYKASPAFQAMIDRYAGSLSVSGLMCKQISFRGDILISKERIEAYFYSLDQQLPIPNRAELTAEWLL
AEISALEKKERKKEWVLQESELLDKEDYAEVYQTLKKQAADDEPAFDDHQREQRLLGAIIVGKAFKPIKQAVRRLGFLDI
KRMYLRLFSDWGADEKPDHWESIGALTRNAFAERSLPYEDAAPYLYLQDLLEGKRKNTQMKHVFIDEAQDYSPFQLTYIQ
SIFPAAKMTVLGDVSQSIYAHTITGPSSLAGCFRNGAEKYVSLKRTYRSTRQIVEFTKALLADGDDIEPFNRNGEKPRVL
RTDTENARQEQLADTIRELRQKGWETVAVICKTAAEAKKVFQAVSAESGVRLINKENQPFQKGVCVIPVYLAKGIEFDAV
IAADASAVQYKSEHDRKLLYTACTRAMHHLTVLSPGEPSPFIKEVPAGLFV

Nucleotide


Download         Length: 2316 bp        

>NTDB_id=258600 DOU34_RS14715 WP_061047083.1 2980135..2982450(-) (helD) [Bacillus amyloliquefaciens strain SH-B74]
ATGGCAAAACGTGATAAAGAGTGGAAAGAAGAGCAGGAAAGAATCAATTTTGTCATGAAGGAACTGGAACAGAAACAAAC
TCATCTTGAAGCCTCATCGGGCGGATTGAAGCAGGATATTGTCGGGCTGAGAAAAACGTTTTGGGATGATGTCACCGTGA
ATATGGATGACGCCCATGAAGCGGCTGAGACATTTTCAAGCATTAAACAGCAGGCGGAAATGCTTTCAGAACGTGAACGG
ACGCATCGGCTTCAATACCGGCAGCTTCAAAAAATCCAAAAGCTGAAAACCTCTCCTTACTTCGGCCGTGTCGATTTTAC
GGAAAAAGGGGAGTCCTCTTCCGAGCGGATTTATATCGGGCTGACTTCTTTGATGGATGAAAACAACGAGCAATTTCTCA
TTTATGATTGGCGCGCGCCCATCTCATCTCTTTACTATCAATATTCCCCGGGAAAAGCCCGCTATGAAGTTCCCGAAGAA
ACGATAGAAGGAGACATCACACTGAAGCGGCAGTTTATCATTAAAAACGGGACGCTTAAAGCGATGTTTAACACGGATAT
GACGATTGGTGACGAAATGCTGCAAGAGGTGCTGAGCGGGCATTCCGGACAGCATATGAAAAACATCGTGTCAACCATCC
AAAAAGAACAGAACGAAATCATCCGGGATGAAAAAAGCAAATATCTCATCGTTCAGGGAGCCGCCGGAAGCGGAAAAACA
TCCGCGGCGCTTCAGCGGGCCGCTTACTTGCTGTACCGCGGTCGCGGGGTGCTTGATGCCCGTCAGATTGTATTGTTTTC
CCCGAACATTTTGTTCAACAGTTATGTTTCCGCCGTATTGCCGGAATTGGGAGAAGAGAATATGGAGCAGGCCACCTTTC
AGGAATATATCCTCCGCCGGCTCGGACGAAAGTTTGATTGTGAAAGCCCGTACGAGCAGCTTGAATATTGCCTGACTGAA
CAAAATCAGGACGTACTTTTCGTAAGGCTCGCAGCCATTGCTTACAAAGCGTCACCAGCTTTCCAGGCCATGATTGACCG
TTATGCCGGATCGCTTTCGGTCTCCGGCCTGATGTGTAAACAGATCAGCTTCCGGGGAGACATCCTGATTTCAAAAGAGC
GGATTGAAGCCTATTTTTATTCGCTTGATCAGCAGCTGCCGATTCCGAACAGAGCGGAGCTGACGGCGGAATGGCTTCTC
GCGGAAATCAGCGCTCTCGAAAAAAAGGAGCGTAAGAAGGAATGGGTCTTGCAGGAGAGCGAGCTGCTTGATAAGGAAGA
CTATGCTGAGGTTTATCAAACACTCAAGAAGCAGGCGGCAGATGATGAACCTGCATTTGACGACCATCAGCGCGAACAGC
GGCTGCTTGGCGCCATTATTGTCGGCAAAGCCTTTAAACCGATTAAGCAGGCCGTCAGACGGCTCGGCTTTCTCGATATA
AAACGGATGTATCTCCGGCTGTTTTCAGATTGGGGAGCTGATGAAAAGCCCGATCATTGGGAGAGCATCGGCGCTTTGAC
CAGAAACGCATTTGCAGAACGGTCACTTCCGTATGAAGATGCCGCTCCGTATCTGTATTTGCAGGATCTGTTAGAAGGAA
AAAGAAAAAACACACAAATGAAGCACGTCTTCATTGATGAAGCTCAGGATTATTCCCCGTTTCAGCTCACGTATATTCAA
AGCATCTTCCCGGCGGCAAAGATGACGGTGCTCGGTGATGTCAGCCAGTCGATTTATGCCCACACCATTACGGGCCCTTC
TTCCCTCGCCGGCTGCTTTCGCAATGGTGCAGAAAAGTATGTCAGCCTGAAGCGGACTTACAGATCGACGCGGCAGATTG
TTGAATTTACGAAAGCGCTGCTGGCTGACGGCGATGACATTGAGCCTTTTAACCGGAACGGGGAAAAACCGCGTGTTTTG
CGGACGGATACAGAAAATGCGCGGCAGGAACAATTGGCGGATACAATCCGGGAGCTCAGACAAAAAGGCTGGGAGACGGT
CGCCGTCATCTGCAAAACGGCCGCGGAAGCCAAAAAAGTGTTTCAGGCAGTCAGCGCCGAGAGCGGTGTCCGCTTGATCA
ATAAAGAAAACCAGCCTTTTCAAAAAGGGGTATGCGTCATTCCCGTTTATTTGGCAAAAGGCATTGAGTTTGACGCCGTC
ATTGCCGCTGACGCCTCTGCTGTCCAGTACAAAAGCGAACATGACCGTAAGCTGTTATATACGGCCTGTACGCGGGCGAT
GCATCATCTGACCGTTTTGTCTCCGGGAGAACCCTCTCCTTTTATAAAAGAAGTGCCCGCCGGCTTATTCGTGTAA

Domains

Predicted by InterProScan.

UvrD-like helicase C-terminal domain (UvrD_C)

(601-686)

UvrD/REP helicase N-terminal domain (UvrD-helicase)

(415-580)

UvrD-like helicase C-terminal domain (UvrD_C_2)

(704-752)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  helD Bacillus subtilis subsp. subtilis str. 168

66.883

99.87

0.668