Detailed information    

insolico Bioinformatically predicted

Overview


Name   pilH   Type   Machinery gene
Locus tag   DOU34_RS07180 Genome accession   NZ_CP030097
Coordinates   1403906..1404268 (+) Length   120 a.a.
NCBI ID   WP_003154238.1    Uniprot ID   A0A150FA43
Organism   Bacillus amyloliquefaciens strain SH-B74     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context


Location: 1398906..1409268
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DOU34_RS07150 flgD 1399827..1400267 (+) 441 WP_007409782.1 flagellar hook assembly protein FlgD -
  DOU34_RS07155 flgG 1400292..1401068 (+) 777 WP_007409783.1 flagellar basal body rod protein FlgG -
  DOU34_RS07160 - 1401108..1401323 (+) 216 WP_003154243.1 flagellar FlbD family protein -
  DOU34_RS07165 fliL 1401320..1401739 (+) 420 WP_061046787.1 flagellar basal body-associated protein FliL -
  DOU34_RS07170 fliM 1401773..1402771 (+) 999 WP_003154240.1 flagellar motor switch protein FliM -
  DOU34_RS07175 fliY 1402761..1403879 (+) 1119 WP_061046786.1 flagellar motor switch phosphatase FliY -
  DOU34_RS07180 pilH 1403906..1404268 (+) 363 WP_003154238.1 chemotaxis protein CheY Machinery gene
  DOU34_RS07185 fliZ 1404283..1404936 (+) 654 WP_020955822.1 flagella biosynthesis regulatory protein FliZ -
  DOU34_RS07190 fliP 1404929..1405594 (+) 666 WP_007409786.1 flagellar type III secretion system pore protein FliP -
  DOU34_RS07195 fliQ 1405609..1405878 (+) 270 WP_003154234.1 flagellar biosynthesis protein FliQ -
  DOU34_RS07200 fliR 1405885..1406664 (+) 780 WP_061046785.1 flagellar biosynthetic protein FliR -
  DOU34_RS07205 flhB 1406664..1407743 (+) 1080 WP_041482435.1 flagellar biosynthesis protein FlhB -

Sequence


Protein


Download         Length: 120 a.a.        Molecular weight: 13209.53 Da        Isoelectric Point: 4.6805

>NTDB_id=258545 DOU34_RS07180 WP_003154238.1 1403906..1404268(+) (pilH) [Bacillus amyloliquefaciens strain SH-B74]
MAHRILIVDDAAFMRMMIKDILVKNGFDVVAEASDGAQAVEKFKEHSPDLVTMDITMPEMDGITALKEIKQIDPQAKIIM
CSAMGQQSMVIDAIQAGAKDFIVKPFQADRVLEAINKTLS

Nucleotide


Download         Length: 363 bp        

>NTDB_id=258545 DOU34_RS07180 WP_003154238.1 1403906..1404268(+) (pilH) [Bacillus amyloliquefaciens strain SH-B74]
ATGGCACACAGAATTTTAATTGTAGACGATGCAGCATTTATGAGAATGATGATCAAAGATATTTTAGTGAAGAACGGTTT
TGACGTTGTAGCTGAAGCTTCAGACGGAGCGCAGGCAGTCGAAAAATTCAAAGAGCATTCCCCCGACCTTGTGACAATGG
ATATTACAATGCCTGAAATGGACGGGATTACAGCTTTAAAGGAAATTAAACAAATCGATCCGCAGGCGAAAATCATCATG
TGTTCGGCTATGGGCCAGCAGTCAATGGTTATTGACGCGATTCAAGCCGGCGCAAAAGACTTTATCGTGAAACCGTTCCA
AGCGGACCGCGTGCTTGAAGCTATTAATAAAACATTAAGCTAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  AlphaFold DB A0A150FA43

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilH Synechocystis sp. PCC 6803

38.983

98.333

0.383