Detailed information    

insolico Bioinformatically predicted

Overview

Name   recU   Type   Machinery gene
Locus tag   CU648_RS22715 Genome accession   NZ_CP025122
Coordinates   4181618..4182220 (-) Length   200 a.a.
NCBI ID   WP_000155594.1    Uniprot ID   Q81SS2
Organism   Bacillus sp. HBCD-sjtu     
Function   plasmid transformation; homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 4176618..4187220
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CU648_RS22695 (CU648_22695) dnaD 4176943..4177650 (+) 708 WP_000728549.1 DNA replication protein DnaD -
  CU648_RS22700 (CU648_22700) nth 4177666..4178313 (+) 648 WP_000933025.1 endonuclease III -
  CU648_RS22705 (CU648_22705) - 4178317..4178835 (+) 519 WP_017561031.1 YpoC family protein -
  CU648_RS22710 (CU648_22710) - 4178859..4181552 (-) 2694 WP_101195577.1 PBP1A family penicillin-binding protein -
  CU648_RS22715 (CU648_22715) recU 4181618..4182220 (-) 603 WP_000155594.1 Holliday junction resolvase RecU Machinery gene
  CU648_RS22720 (CU648_22720) - 4182324..4183349 (+) 1026 WP_070171200.1 DUF2515 domain-containing protein -
  CU648_RS28650 (CU648_22725) - 4183473..4183649 (-) 177 WP_001982922.1 hypothetical protein -
  CU648_RS22730 (CU648_22730) - 4183735..4183977 (+) 243 WP_001252043.1 hypothetical protein -
  CU648_RS22735 (CU648_22735) - 4183993..4184337 (+) 345 WP_000872959.1 YppE family protein -
  CU648_RS22740 (CU648_22740) - 4184363..4184554 (-) 192 WP_000242663.1 YppF family protein -
  CU648_RS22745 (CU648_22745) - 4184732..4185211 (+) 480 WP_000487704.1 YppG family protein -
  CU648_RS22750 (CU648_22750) - 4185367..4185795 (+) 429 WP_000546294.1 CotD family spore coat protein -
  CU648_RS22755 (CU648_22755) - 4185852..4186406 (+) 555 WP_000862921.1 DUF1273 domain-containing protein -
  CU648_RS22760 (CU648_22760) gpsB 4186504..4186842 (+) 339 WP_000622430.1 cell division regulator GpsB -

Sequence

Protein


Download         Length: 200 a.a.        Molecular weight: 23342.66 Da        Isoelectric Point: 9.5601

>NTDB_id=258519 CU648_RS22715 WP_000155594.1 4181618..4182220(-) (recU) [Bacillus sp. HBCD-sjtu]
MTIRYPNGKRYNQASQPHKTPIKKHTYSNRGMSLEEELNETNEYYLTHNIACVHKKPTPLQIVKVDYPARSAAVVKEAYF
KQPSTTDYNGVYKGKYIDFEAKETKNKTSFPLQNFHLHQIEHMKQVIAHNGIAFVIIKFTLFDELYLLDAKHIITFWNRQ
NTGGRKSITKEEIVEHGSLLSCGYHPRIDYIRVLDTVYFS

Nucleotide


Download         Length: 603 bp        

>NTDB_id=258519 CU648_RS22715 WP_000155594.1 4181618..4182220(-) (recU) [Bacillus sp. HBCD-sjtu]
ATGACCATTCGTTACCCAAATGGAAAACGGTACAATCAAGCTTCACAACCTCATAAAACACCAATTAAAAAACATACTTA
TAGTAACAGAGGTATGTCCCTTGAAGAGGAATTGAATGAAACAAATGAATATTACTTAACCCATAATATTGCATGTGTAC
ATAAAAAACCTACACCTCTTCAAATTGTAAAAGTAGATTACCCCGCTCGAAGTGCTGCAGTGGTAAAAGAGGCGTATTTT
AAACAACCTTCTACAACAGATTACAACGGTGTATATAAAGGGAAATACATCGATTTTGAAGCGAAAGAAACAAAAAATAA
AACCAGCTTTCCACTTCAAAACTTCCACCTTCATCAAATTGAACATATGAAGCAAGTAATTGCTCATAATGGAATTGCAT
TTGTTATTATTAAATTTACACTTTTTGATGAACTTTATTTATTAGATGCAAAACATATTATTACATTTTGGAATCGTCAA
AATACTGGTGGACGAAAATCGATTACGAAAGAAGAAATAGTAGAGCATGGATCTTTATTATCATGCGGTTATCACCCTCG
GATTGATTATATCCGTGTACTAGACACGGTTTATTTTTCGTGA

Domains

Predicted by InterproScan.

Recombination protein U (RecU)

(30-195)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q81SS2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recU Bacillus subtilis subsp. subtilis str. 168

62.5

100

0.625

Multiple sequence alignment