Detailed information    

insolico Bioinformatically predicted

Overview

Name   rapF   Type   Regulator
Locus tag   DOU34_RS00110 Genome accession   NZ_CP030097
Coordinates   18867..19988 (+) Length   373 a.a.
NCBI ID   WP_007409040.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain SH-B74     
Function   inhibit the DNA-binding function of ComA (predicted from homology)   
Competence regulation

Genomic Context

Location: 13867..24988
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DOU34_RS00090 - 14178..15172 (-) 995 Protein_16 DUF5105 domain-containing protein -
  DOU34_RS00095 - 15299..16699 (+) 1401 WP_061047164.1 YcdB/YcdC domain-containing protein -
  DOU34_RS00100 - 16900..18291 (+) 1392 WP_113629785.1 YcdB/YcdC domain-containing protein -
  DOU34_RS00105 - 18254..18757 (-) 504 WP_061047166.1 M15 family metallopeptidase -
  DOU34_RS00110 rapF 18867..19988 (+) 1122 WP_007409040.1 tetratricopeptide repeat protein Regulator
  DOU34_RS00115 - 20104..20882 (+) 779 Protein_21 glucose 1-dehydrogenase -
  DOU34_RS00120 - 20965..22641 (+) 1677 WP_032867307.1 alpha-glucosidase -
  DOU34_RS00125 - 22827..23786 (+) 960 WP_032867360.1 metal ABC transporter substrate-binding protein -
  DOU34_RS00130 - 23841..24536 (+) 696 WP_021495283.1 metal ABC transporter ATP-binding protein -

Sequence

Protein


Download         Length: 373 a.a.        Molecular weight: 44333.53 Da        Isoelectric Point: 5.0806

>NTDB_id=258488 DOU34_RS00110 WP_007409040.1 18867..19988(+) (rapF) [Bacillus amyloliquefaciens strain SH-B74]
MKSKIPSEEVAVKMNEWYKLIRAFEADQAEALKREIQYELEDMEENQDLLLYFSLMEFRHRIMLDKLKPMKDGDCQPPFS
EMLTEIEMNQQKLTGLLEYYFYYFRGMYEFKQKNFISAISHYKRAEEKLEYVEDDIEKAEFLYKISEVYYHIKQTFFSMN
YAGQALDIYKKYELYGMRRVQCKFIIAGNLMDAQQHKKALEYLECALEHAERLDDQYMIAAAYYNIGHCMYSLGDFCKAA
EDFERAAAGFEAHSFRQVIQAVFSLTHIYFKTEKYSEAAASCARGMRSAAEWEDEIYMTKFRLIKELYQGEGGQDSLKEY
FDILESKELLADTEELLQDAAHFFHKRENYKAASYFFSRLVGVQKRLQMNNYA

Nucleotide


Download         Length: 1122 bp        

>NTDB_id=258488 DOU34_RS00110 WP_007409040.1 18867..19988(+) (rapF) [Bacillus amyloliquefaciens strain SH-B74]
ATGAAATCCAAGATTCCATCTGAAGAAGTGGCCGTAAAGATGAATGAATGGTATAAGCTGATCCGCGCGTTTGAAGCCGA
TCAAGCTGAAGCGCTGAAGCGGGAGATTCAATATGAACTCGAAGATATGGAAGAGAACCAGGATTTATTGTTGTATTTTT
CATTGATGGAATTTCGGCACCGCATCATGCTGGATAAATTAAAACCCATGAAAGACGGCGACTGCCAGCCTCCGTTCTCT
GAAATGCTCACTGAGATTGAGATGAACCAGCAAAAGCTGACCGGCTTACTGGAATATTATTTTTACTATTTCAGGGGAAT
GTATGAATTTAAGCAGAAAAACTTCATCTCAGCGATTTCTCATTATAAGCGGGCTGAGGAAAAGCTTGAATATGTGGAAG
ATGACATAGAGAAAGCCGAGTTTCTGTATAAAATTTCTGAGGTGTACTATCACATCAAGCAGACTTTCTTTTCGATGAAT
TACGCGGGGCAGGCGCTTGATATTTACAAGAAATATGAATTATACGGAATGAGAAGGGTGCAGTGCAAATTTATCATTGC
CGGAAATCTGATGGACGCCCAGCAGCATAAAAAAGCGCTGGAATACCTCGAATGCGCCTTGGAGCACGCGGAGCGGCTTG
ATGATCAATATATGATTGCTGCGGCCTATTATAATATCGGGCACTGTATGTACAGTCTGGGTGACTTTTGCAAGGCGGCG
GAGGATTTTGAACGGGCGGCTGCCGGATTTGAAGCACACAGCTTCCGGCAGGTCATCCAGGCTGTTTTTTCACTCACGCA
TATCTACTTTAAAACGGAAAAATACAGTGAAGCCGCCGCCAGCTGCGCGCGGGGCATGAGAAGCGCGGCGGAGTGGGAAG
ATGAGATCTATATGACGAAATTCCGTCTGATTAAAGAGCTTTATCAGGGGGAAGGCGGACAGGATTCACTCAAAGAGTAT
TTTGACATTCTGGAATCAAAAGAGCTGTTGGCTGACACAGAGGAACTGCTTCAGGACGCGGCTCATTTCTTTCATAAACG
GGAGAACTACAAAGCGGCATCTTATTTTTTCAGCAGGCTTGTCGGCGTGCAAAAAAGGCTGCAAATGAACAACTACGCAT
AA

Domains

Predicted by InterProScan.

Tetratricopeptide repeat (TPR_12)

(187-251)

response regulator aspartate phosphatase H, N terminal (RapH_N)

(4-65)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rapF Bacillus subtilis subsp. subtilis str. 168

42.588

99.464

0.424
  rapC Bacillus subtilis subsp. subtilis str. 168

40.323

99.732

0.402