Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   CU648_RS07735 Genome accession   NZ_CP025122
Coordinates   1415869..1416912 (-) Length   347 a.a.
NCBI ID   WP_046946783.1    Uniprot ID   -
Organism   Bacillus sp. HBCD-sjtu     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1410869..1421912
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CU648_RS29040 - 1410928..1411032 (+) 105 Protein_1479 IS3 family transposase -
  CU648_RS07690 (CU648_07690) - 1411296..1411496 (-) 201 WP_000106083.1 YqzE family protein -
  CU648_RS07695 (CU648_07695) aroK 1411535..1412032 (-) 498 WP_000836631.1 shikimate kinase AroK -
  CU648_RS07700 (CU648_07700) - 1412153..1412803 (-) 651 WP_149884809.1 2OG-Fe(II) oxygenase -
  CU648_RS07705 (CU648_07705) comGG 1412979..1413350 (-) 372 WP_101194881.1 competence type IV pilus minor pilin ComGG -
  CU648_RS07710 (CU648_07710) comGF 1413347..1413817 (-) 471 WP_000923093.1 competence type IV pilus minor pilin ComGF -
  CU648_RS07715 (CU648_07715) comGE 1413787..1414089 (-) 303 WP_046946785.1 competence type IV pilus minor pilin ComGE -
  CU648_RS07720 (CU648_07720) comGD 1414082..1414537 (-) 456 WP_000810395.1 comG operon protein ComGD -
  CU648_RS07725 (CU648_07725) comGC 1414534..1414833 (-) 300 WP_001178696.1 comG operon protein ComGC -
  CU648_RS07730 (CU648_07730) comGB 1414845..1415876 (-) 1032 WP_017560029.1 comG operon protein ComGB -
  CU648_RS07735 (CU648_07735) comGA 1415869..1416912 (-) 1044 WP_046946783.1 competence protein ComGA Machinery gene
  CU648_RS07740 (CU648_07740) - 1417118..1417813 (+) 696 WP_101194882.1 metalloregulator ArsR/SmtB family transcription factor -
  CU648_RS07745 (CU648_07745) - 1417939..1418181 (+) 243 WP_000440711.1 DUF2626 domain-containing protein -
  CU648_RS07750 (CU648_07750) - 1418289..1419683 (+) 1395 WP_001094329.1 L-cystine transporter -
  CU648_RS07755 (CU648_07755) - 1419859..1420266 (-) 408 WP_017560026.1 hypothetical protein -
  CU648_RS07760 (CU648_07760) - 1420354..1420569 (-) 216 WP_001008320.1 DUF3912 family protein -
  CU648_RS07765 (CU648_07765) - 1420822..1421133 (+) 312 WP_001093243.1 hypothetical protein -
  CU648_RS07770 (CU648_07770) - 1421170..1421658 (-) 489 WP_000764253.1 hypothetical protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 39332.82 Da        Isoelectric Point: 9.0775

>NTDB_id=258483 CU648_RS07735 WP_046946783.1 1415869..1416912(-) (comGA) [Bacillus sp. HBCD-sjtu]
MNGIESFANTILKEACRVQASDLHIVPRQKDVVVQLRIGKDLMTKQCIEKEFGEKLVSHFKFLASMDIGERRKPQNGSLY
LQMDGQEVYLRLSTLPTVYQESLVIRLHLQASIQPLSHLSLFPSTAKILLSFLRYSHGLLVFTGPTGSGKTTTMYALLEV
IRKKKTRRIVTLEDPVEKRNDDVLQIQINEKAGITYEAGLKAILRHDPDVILVGEIRDEETAKIAIRASLTGHLVMTTLH
TNDARGAILRFMDFGITRQEIEQSLLAIAAQRLVELKCPFCKRKCSTLCKSMRQVRQASIYELLYGYELKQAIKEANGEC
VTYKHETLQSSIRKGYALGFLEEDVYV

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=258483 CU648_RS07735 WP_046946783.1 1415869..1416912(-) (comGA) [Bacillus sp. HBCD-sjtu]
ATGAATGGAATTGAAAGCTTTGCGAATACGATTTTGAAAGAAGCGTGCAGGGTGCAAGCGTCGGACTTACATATTGTACC
CCGGCAGAAGGATGTAGTCGTTCAACTGCGTATAGGAAAAGATTTAATGACGAAACAATGCATTGAAAAGGAGTTTGGAG
AAAAACTTGTTTCACACTTTAAATTTTTAGCATCCATGGATATAGGGGAGAGGCGGAAGCCACAAAATGGTTCACTGTAT
TTACAAATGGATGGACAGGAAGTGTATTTGCGCCTTTCCACGCTTCCAACCGTATACCAAGAAAGTCTCGTTATTCGTCT
TCATTTACAAGCATCTATTCAGCCGTTATCTCATCTTTCGTTATTTCCAAGTACAGCGAAGATACTACTTTCTTTTTTAC
GTTATTCTCATGGATTACTCGTATTTACTGGACCGACTGGTTCGGGGAAGACAACAACAATGTATGCATTATTAGAGGTA
ATTAGAAAAAAGAAAACACGTCGCATCGTTACACTGGAAGATCCAGTTGAAAAAAGAAATGACGATGTATTACAAATTCA
AATAAATGAAAAAGCAGGTATCACATATGAGGCTGGACTAAAGGCTATTTTGCGTCATGATCCAGATGTTATTTTAGTCG
GTGAAATTCGTGATGAAGAAACAGCGAAAATTGCTATAAGAGCAAGTTTGACTGGCCATTTAGTAATGACGACATTGCAT
ACGAATGATGCGAGAGGGGCGATACTTCGGTTCATGGATTTTGGCATAACGAGGCAAGAAATCGAACAATCTTTATTAGC
TATAGCTGCACAGCGACTTGTCGAATTAAAGTGTCCGTTTTGCAAAAGAAAGTGCTCAACTTTATGCAAATCAATGAGGC
AAGTAAGGCAAGCGAGTATTTATGAGTTGTTATATGGATATGAGTTAAAACAAGCGATTAAAGAAGCAAATGGGGAATGT
GTCACATACAAGCACGAAACATTACAATCTTCGATACGAAAAGGATACGCTTTAGGGTTTTTAGAAGAAGATGTTTATGT
TTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

57.925

100

0.579
  pilB Vibrio campbellii strain DS40M4

35.511

100

0.36

Multiple sequence alignment