Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   CU648_RS06395 Genome accession   NZ_CP025122
Coordinates   1200690..1201124 (-) Length   144 a.a.
NCBI ID   WP_000811492.1    Uniprot ID   A0A0G8EA73
Organism   Bacillus sp. HBCD-sjtu     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1195690..1206124
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CU648_RS06375 (CU648_06375) - 1196454..1197011 (+) 558 WP_017561143.1 PadR family transcriptional regulator -
  CU648_RS06385 (CU648_06385) metE 1197939..1200227 (+) 2289 WP_001007635.1 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase -
  CU648_RS06390 (CU648_06390) comJ 1200264..1200653 (-) 390 WP_000424033.1 competence protein ComJ -
  CU648_RS06395 (CU648_06395) nucA/comI 1200690..1201124 (-) 435 WP_000811492.1 DNA-entry nuclease Machinery gene
  CU648_RS06405 (CU648_06405) - 1201361..1202110 (+) 750 WP_000388464.1 DUF3967 domain-containing protein -
  CU648_RS06410 (CU648_06410) kinB 1202164..1203438 (-) 1275 WP_000420390.1 sporulation sensor histidine kinase KinB -
  CU648_RS06415 (CU648_06415) - 1203749..1203949 (+) 201 WP_000929284.1 hypothetical protein -
  CU648_RS06420 (CU648_06420) - 1204072..1205235 (+) 1164 WP_000434590.1 aminotransferase A -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 16012.35 Da        Isoelectric Point: 8.4331

>NTDB_id=258480 CU648_RS06395 WP_000811492.1 1200690..1201124(-) (nucA/comI) [Bacillus sp. HBCD-sjtu]
MKQLKGIIISIIAILSILVAVYEVLVPEETSTKKTNAYDQVLEFPKERYPETGKHITDAIKEGHSEVCTIDRGGAADRRK
LSLAPYPSKKGYDRDEWPMAMCKEGGKGAHIEYISPADNRGAGSWVGNKLDKYPDGTRVKFDVK

Nucleotide


Download         Length: 435 bp        

>NTDB_id=258480 CU648_RS06395 WP_000811492.1 1200690..1201124(-) (nucA/comI) [Bacillus sp. HBCD-sjtu]
ATGAAGCAATTAAAAGGTATTATCATTTCAATTATTGCAATTCTTTCTATTTTAGTAGCGGTTTATGAAGTACTTGTTCC
AGAGGAAACAAGTACAAAAAAAACAAATGCGTATGATCAAGTTCTAGAATTTCCGAAAGAGAGATATCCAGAAACAGGGA
AACATATTACGGATGCGATAAAGGAAGGACATTCAGAAGTTTGTACAATCGACCGTGGTGGCGCTGCGGATAGAAGAAAG
TTATCGTTAGCTCCATACCCATCAAAAAAAGGGTATGATCGTGATGAATGGCCAATGGCGATGTGTAAAGAAGGCGGAAA
AGGAGCACATATCGAATATATAAGTCCAGCAGATAACCGCGGAGCAGGGTCTTGGGTAGGAAATAAGTTAGATAAATACC
CAGATGGTACGCGTGTGAAATTTGACGTAAAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(48-139)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0G8EA73

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

70.796

78.472

0.556

Multiple sequence alignment