Detailed information    

insolico Bioinformatically predicted

Overview

Name   recO   Type   Machinery gene
Locus tag   CWI35_RS15995 Genome accession   NZ_CP025074
Coordinates   3081831..3082619 (+) Length   262 a.a.
NCBI ID   WP_011231966.1    Uniprot ID   -
Organism   [Bacillus] caldolyticus strain NEB414     
Function   ssDNA annealing; plasmid transformation (predicted from homology)   
DNA processing Homologous recombination

Genomic Context

Location: 3076831..3087619
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CWI35_RS15965 (CWI35_15935) - 3077209..3079311 (+) 2103 WP_011231972.1 HD family phosphohydrolase -
  CWI35_RS15970 (CWI35_15940) ybeY 3079308..3079778 (+) 471 WP_011231971.1 rRNA maturation RNase YbeY -
  CWI35_RS15975 (CWI35_15945) - 3079778..3080131 (+) 354 WP_011231970.1 diacylglycerol kinase family protein -
  CWI35_RS15980 (CWI35_15950) - 3080255..3080653 (+) 399 WP_021322326.1 cytidine deaminase -
  CWI35_RS15985 (CWI35_15955) era 3080646..3081554 (+) 909 WP_011231968.1 GTPase Era -
  CWI35_RS15990 (CWI35_15960) - 3081656..3081799 (+) 144 WP_011231967.1 YqzL family protein -
  CWI35_RS15995 (CWI35_15965) recO 3081831..3082619 (+) 789 WP_011231966.1 DNA repair protein RecO Machinery gene
  CWI35_RS16000 (CWI35_15970) - 3082722..3083315 (+) 594 WP_011231965.1 helix-turn-helix transcriptional regulator -
  CWI35_RS16005 (CWI35_15975) - 3083338..3084138 (+) 801 WP_014196367.1 pyruvate, water dikinase regulatory protein -
  CWI35_RS16010 (CWI35_15980) dnaG 3084672..3086468 (+) 1797 WP_119877919.1 DNA primase -

Sequence

Protein


Download         Length: 262 a.a.        Molecular weight: 29403.00 Da        Isoelectric Point: 8.3177

>NTDB_id=258048 CWI35_RS15995 WP_011231966.1 3081831..3082619(+) (recO) [[Bacillus] caldolyticus strain NEB414]
MFETCEAIVMRTVDYGETNKIVTLFTREWGKVAAMARGAKKPSSRLSAVTQPLSYGHYVIRRSRGVGVLHQGELIDSMRA
LREDLFAAAYAVYIVELTDKSTEEQKRNPYLFELLLQTLQYMSEGRDLEILTFIYEMKMLAVLGIPPVLDRCARCGATEG
RFSFSVKEAGFLCHRCEASDPHRFPLSSASVRLLRLFFHIDLARLGSISVKESTKMELGAVLSAYYDEYAGLSLKTKRFL
QQIGELKDKLSPDGGQGASFSL

Nucleotide


Download         Length: 789 bp        

>NTDB_id=258048 CWI35_RS15995 WP_011231966.1 3081831..3082619(+) (recO) [[Bacillus] caldolyticus strain NEB414]
ATGTTCGAAACGTGTGAAGCGATCGTCATGCGGACGGTCGATTATGGCGAAACAAATAAAATTGTCACATTGTTTACCCG
AGAATGGGGAAAGGTGGCTGCGATGGCAAGGGGAGCGAAAAAGCCAAGCAGCCGCCTTTCTGCTGTTACGCAGCCGCTCT
CATATGGCCATTATGTCATCCGCCGCAGCCGCGGCGTCGGCGTTCTTCACCAGGGAGAGCTCATCGACTCGATGCGGGCG
CTGCGCGAAGATTTGTTTGCCGCCGCCTATGCGGTGTATATTGTAGAGCTCACCGACAAAAGCACGGAAGAGCAAAAGCG
CAATCCGTACTTGTTTGAGCTTCTTTTGCAGACACTACAATATATGAGCGAAGGCCGCGATTTAGAGATTTTGACGTTCA
TTTACGAAATGAAAATGCTTGCAGTGCTTGGCATTCCGCCGGTGCTGGACCGTTGTGCCCGCTGCGGGGCGACGGAGGGG
CGCTTCTCGTTTTCCGTCAAGGAGGCGGGCTTCCTTTGCCATCGCTGCGAGGCGTCCGATCCCCATCGGTTCCCGCTCTC
TTCGGCTTCCGTCCGGCTGTTGCGCCTCTTTTTTCATATCGATCTTGCTCGGCTTGGATCCATATCGGTGAAAGAAAGCA
CAAAGATGGAGCTGGGCGCCGTATTGTCCGCCTATTATGATGAATATGCCGGCCTGTCGCTGAAGACGAAGCGTTTTTTG
CAGCAAATCGGTGAACTGAAAGACAAGCTCAGCCCCGACGGAGGGCAAGGAGCATCATTTTCATTGTGA

Domains

Predicted by InterproScan.

Recombination protein O C terminal (RecO_C)

(82-237)

Recombination protein O N terminal (RecO_N)

(1-77)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recO Bacillus subtilis subsp. subtilis str. 168

56.275

94.275

0.531

Multiple sequence alignment