Detailed information NTG0258027

Bioinformatically predicted

Overview

• Name: recF
• Type: Machinery gene
• Locus tag: CWI35_RS10550
• Genome accession: NZ_CP025074
• Coordinates: 2020745..2021863 (-)
• Length: 372 a.a.
• NCBI ID: WP_011229520.1
• Uniprot ID: -
• Organism: [Bacillus] caldolyticus strain NEB414
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 2015745..2026863

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
CWI35_RS10540 (CWI35_10520)	gyrA	2016169..2018625 (-)	2457	WP_011229522.1	DNA gyrase subunit A	-
CWI35_RS10545 (CWI35_10525)	gyrB	2018776..2020692 (-)	1917	WP_011229521.1	DNA topoisomerase (ATP-hydrolyzing) subunit B	-
CWI35_RS10550 (CWI35_10530)	recF	2020745..2021863 (-)	1119	WP_011229520.1	DNA replication/repair protein RecF	Machinery gene
CWI35_RS10555 (CWI35_10535)	yaaA	2021875..2022105 (-)	231	WP_012820418.1	S4 domain-containing protein YaaA	-
CWI35_RS10560 (CWI35_10540)	dnaN	2022294..2023430 (-)	1137	WP_011229518.1	DNA polymerase III subunit beta	-
CWI35_RS10565 (CWI35_10545)	dnaA	2023603..2024955 (-)	1353	WP_011229517.1	chromosomal replication initiator protein DnaA	-
CWI35_RS10570 (CWI35_10550)	rpmH	2025434..2025568 (+)	135	WP_003253900.1	50S ribosomal protein L34	-
CWI35_RS10575 (CWI35_10555)	rnpA	2025648..2026013 (+)	366	WP_011232960.1	ribonuclease P protein component	-
CWI35_RS10580 (CWI35_10560)	spoIIIJ	2026086..2026850 (+)	765	WP_011232959.1	YidC family membrane integrase SpoIIIJ	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 42582.66 Da
• Isoelectric Point: 6.8368

>NTDB_id=258027 CWI35_RS10550 WP_011229520.1 2020745..2021863(-) (recF) [[Bacillus] caldolyticus strain NEB414]
MFLTNLTLTNYRNYEYETLNFGEGVNVILGENAQGKTNMMEAIYVLAMAKSHRTSNDKDLIRWNEEYAKIEGRAEKRSGS
LTLELLISKKGKKARCNHIEQQRLSQYVGHLNVVMFAPEDLNLVKGSPQVRRRFVDMEIGQVSPVYIHDLSQYQKLLQQR
NHYLKMMQARERSDEAVLDVLTEQLVLLAAKITLRRRQFLSLLEQWAMPIHYEISRGAEQLCIRYEPSVDVSEKAELSRI
VEAYSETFAAMREREVQRGTTLVGPHRDDIAFFVNGKNVQTFGSQGQQRTTALAVKLAELELIFSELGDYPILLLDDVLS
ELDDFRQTHLLDAIRKKVQTFVTTTSIDGIKHDIIQEAAIYRVHSGSVTAPS

Nucleotide

• Length: 1119 bp

>NTDB_id=258027 CWI35_RS10550 WP_011229520.1 2020745..2021863(-) (recF) [[Bacillus] caldolyticus strain NEB414]
GTGTTTTTGACCAACTTAACGTTGACGAATTACCGGAATTATGAGTATGAAACGCTGAATTTTGGTGAGGGCGTGAACGT
CATCTTAGGAGAAAATGCCCAAGGAAAGACGAACATGATGGAGGCCATTTATGTTTTGGCTATGGCGAAGTCGCACCGCA
CGTCCAACGATAAAGACCTTATCCGTTGGAATGAAGAGTATGCTAAAATAGAAGGAAGAGCGGAAAAACGAAGCGGGTCG
TTAACGCTTGAGTTGCTTATTTCAAAAAAAGGAAAAAAAGCAAGATGCAACCATATTGAACAGCAGCGGCTCAGCCAATA
CGTCGGCCATTTGAATGTCGTCATGTTTGCTCCCGAAGACTTGAATTTAGTCAAGGGAAGTCCGCAAGTGCGGCGCCGAT
TTGTCGATATGGAAATCGGGCAAGTGTCGCCTGTGTATATACATGATTTGAGCCAATACCAAAAGCTGCTGCAACAACGC
AATCATTACTTAAAAATGATGCAGGCGCGCGAGCGGAGCGATGAGGCGGTTCTTGATGTGTTAACTGAGCAGCTTGTATT
GCTGGCGGCCAAAATTACGCTGCGGCGCCGCCAGTTTTTGTCGCTGCTTGAGCAATGGGCGATGCCAATCCACTATGAAA
TCAGCCGCGGCGCCGAACAGCTTTGCATCCGATACGAACCGTCGGTCGACGTATCAGAAAAGGCGGAATTGTCGAGAATA
GTAGAAGCATACAGTGAAACGTTCGCCGCCATGAGGGAACGGGAAGTTCAGCGGGGAACAACGCTGGTCGGCCCGCATCG
TGATGACATCGCGTTTTTCGTGAATGGAAAAAACGTGCAAACTTTCGGTTCCCAGGGGCAACAACGTACAACCGCGCTAG
CGGTCAAGCTGGCAGAACTTGAGCTCATCTTTTCCGAGTTAGGCGATTACCCCATTTTATTGCTGGATGATGTGTTGTCA
GAACTTGATGACTTTCGGCAAACCCACCTCCTTGATGCCATCCGGAAAAAAGTGCAAACTTTTGTCACAACAACAAGCAT
TGACGGCATTAAGCACGATATCATTCAAGAAGCGGCTATTTATCGAGTCCATTCTGGTTCGGTAACCGCTCCTTCTTGA

Domains

Predicted by InterproScan.

• RecF/RecN/SMC N terminal domain (SMC_N) (2-346)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
recF	Bacillus subtilis subsp. subtilis str. 168	64.946	98.925	0.642