Detailed information    

insolico Bioinformatically predicted

Overview

Name   radC   Type   Machinery gene
Locus tag   B4O06_RS03305 Genome accession   NZ_CP029981
Coordinates   430356..431024 (+) Length   222 a.a.
NCBI ID   WP_001297375.1    Uniprot ID   P65957
Organism   Escherichia coli strain 99-3165     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 425356..436024
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B4O06_RS03270 yicC 425375..426238 (-) 864 WP_000621336.1 YicC/YloC family endoribonuclease -
  B4O06_RS03280 rph 426365..427081 (+) 717 WP_001247089.1 ribonuclease PH -
  B4O06_RS03285 pyrE 427147..427788 (+) 642 WP_000806177.1 orotate phosphoribosyltransferase -
  B4O06_RS03290 slmA 427825..428421 (-) 597 WP_000818601.1 nucleoid occlusion factor SlmA -
  B4O06_RS03295 dut 428528..428986 (-) 459 WP_000976070.1 dUTP diphosphatase -
  B4O06_RS03300 coaBC 428964..430184 (-) 1221 WP_000050139.1 bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC -
  B4O06_RS03305 radC 430356..431024 (+) 669 WP_001297375.1 DNA repair protein RadC Machinery gene
  B4O06_RS03310 rpmB 431241..431477 (+) 237 WP_000091955.1 50S ribosomal protein L28 -
  B4O06_RS03315 rpmG 431498..431665 (+) 168 WP_001051798.1 50S ribosomal protein L33 -
  B4O06_RS03320 mutM 431763..432572 (+) 810 WP_001114533.1 bifunctional DNA-formamidopyrimidine glycosylase/DNA-(apurinic or apyrimidinic site) lyase -
  B4O06_RS03325 coaD 432611..433090 (-) 480 WP_001171873.1 pantetheine-phosphate adenylyltransferase -
  B4O06_RS03330 waaA 433098..434375 (-) 1278 WP_000891562.1 lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase -
  B4O06_RS03335 rfaQ 434788..435846 (+) 1059 WP_000360402.1 lipopolysaccharide core heptosyltransferase RfaQ -

Sequence

Protein


Download         Length: 222 a.a.        Molecular weight: 25258.35 Da        Isoelectric Point: 7.9748

>NTDB_id=257856 B4O06_RS03305 WP_001297375.1 430356..431024(+) (radC) [Escherichia coli strain 99-3165]
MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTLAKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQL
KGIAELARRYYNVRMREESPLLSPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAERGWI

Nucleotide


Download         Length: 669 bp        

>NTDB_id=257856 B4O06_RS03305 WP_001297375.1 430356..431024(+) (radC) [Escherichia coli strain 99-3165]
GTGAAAAACAATGCACAGCTGTTGATGCCGCGCGAAAAAATGCTGAAGTTTGGTATTAGCGCCTTAACGGATGTCGAGCT
GCTGGCGTTATTTCTGCGTACCGGAACGCGCGGTAAAGATGTATTAACCCTGGCAAAAGAGATGCTGGAGAATTTCGGCT
CTCTTTATGGCTTGTTAACCTCTGAATATGAACAATTTAGTGGCGTTCATGGAATTGGCGTAGCGAAATTTGCCCAGTTA
AAGGGGATTGCTGAACTGGCGCGGCGTTACTACAACGTGCGGATGCGTGAAGAAAGCCCTTTACTCAGCCCGGAGATGAC
GCGGGAATTTTTACAAAGCCAGCTCACGGGTGAGGAGCGGGAGATCTTTATGGTGATCTTTCTCGACTCACAACACCGGG
TTATAACGCATAGCCGTCTTTTTTCCGGCACGCTAAACCATGTTGAAGTCCATCCTCGGGAAATTATCCGCGAAGCGATA
AAAATAAACGCCTCGGCGCTGATCCTTGCACATAATCACCCTTCGGGTTGTGCTGAACCCAGTAAAGCGGATAAACTCAT
TACTGAACGGATAATAAAGAGTTGTCAGTTCATGGATTTACGCGTGCTCGACCATATCGTGATTGGGCGTGGAGAGTATG
TTTCTTTTGCCGAACGCGGCTGGATTTAA

Domains

Predicted by InterProScan.

RadC-like JAB domain (RadC)

(103-220)

UPF0758 N-terminal (UPF0758_N)

(9-77)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P65957

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radC Haemophilus influenzae Rd KW20

49.55

100

0.496

Multiple sequence alignment