Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   RMET_RS01540 Genome accession   NC_007973
Coordinates   318618..320096 (-) Length   492 a.a.
NCBI ID   WP_011515189.1    Uniprot ID   Q1LRN7
Organism   Cupriavidus metallidurans CH34     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 313618..325096
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RMET_RS01510 (Rmet_0296) - 313990..314643 (-) 654 WP_011515184.1 LON peptidase substrate-binding domain-containing protein -
  RMET_RS01515 (Rmet_0297) rapZ 314690..315580 (-) 891 WP_011515185.1 RNase adapter RapZ -
  RMET_RS01520 (Rmet_0298) - 315695..316036 (-) 342 WP_017511023.1 PsiF family protein -
  RMET_RS01525 (Rmet_0299) hprK 316242..317213 (-) 972 WP_008650955.1 HPr(Ser) kinase/phosphatase -
  RMET_RS01530 (Rmet_0300) ptsN 317282..317737 (-) 456 WP_017511024.1 PTS IIA-like nitrogen regulatory protein PtsN -
  RMET_RS01535 (Rmet_0302) hpf 318214..318567 (-) 354 WP_008650960.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  RMET_RS01540 (Rmet_0303) rpoN/rpoN1 318618..320096 (-) 1479 WP_011515189.1 RNA polymerase factor sigma-54 Machinery gene
  RMET_RS01545 (Rmet_0304) lptB 320220..321011 (-) 792 WP_011515190.1 LPS export ABC transporter ATP-binding protein -
  RMET_RS01550 (Rmet_0305) lptA 321037..321681 (-) 645 WP_011515191.1 lipopolysaccharide transport periplasmic protein LptA -
  RMET_RS01555 (Rmet_0306) lptC 321744..322370 (-) 627 WP_011515192.1 LPS export ABC transporter periplasmic protein LptC -
  RMET_RS01560 (Rmet_0307) - 322374..323357 (-) 984 WP_011515193.1 SIS domain-containing protein -

Sequence

Protein


Download         Length: 492 a.a.        Molecular weight: 54773.03 Da        Isoelectric Point: 4.9624

>NTDB_id=25779 RMET_RS01540 WP_011515189.1 318618..320096(-) (rpoN/rpoN1) [Cupriavidus metallidurans CH34]
MKPSLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVEQALTENPLLERENDWIESPLRVAADGSVNVQSAPAPASSEPQ
GNGDDRANGANGEDDSYGDSAGDDYGSDWSLDDFARRPQGDDDEKTPMQLREAEPTLREYLMEQLTPLKISTRDKGLAIF
LIESLDDDGYLGATLDEICSELPKELEFEVDEVQAILTLLQSFDPPGVGARNAAECLTLQLRRLQHPQRDLALSIVNHHL
ELLAVRDYTRLKKALQVDEIALKAAHDLIRSLAPYPGHAYSRPEADFVVPDVFVRKSGSSWIAQLNPDVMPRLRINDMYA
QILRGAKGEAGAAGLQQKLQEARWLIKNIQQRFDTILRVAQAIVERQRNFFSHGEIAMRPLVLREIADTLGLHESTISRV
TTNKYMATPMGTFELKYFFGSHVSTETGGAASSTAIRALIKQLIGAEEPRNPLSDSRIAELLGEQGFVVARRTVAKYREA
LKIPAVNLRKTL

Nucleotide


Download         Length: 1479 bp        

>NTDB_id=25779 RMET_RS01540 WP_011515189.1 318618..320096(-) (rpoN/rpoN1) [Cupriavidus metallidurans CH34]
ATGAAACCATCGCTTCAGCTTCGACTTTCCCAGCATCTGGCTCTGACTCCTCAACTGCAGCAGTCCATCCGGCTGCTGCA
GTTATCCACACTGGAGCTGCAACAGGAAGTCGAGCAGGCGCTGACCGAGAATCCACTGCTCGAACGCGAGAACGACTGGA
TCGAAAGCCCGCTGCGCGTGGCCGCGGACGGGTCGGTCAACGTGCAAAGCGCCCCGGCGCCAGCATCTTCCGAACCGCAA
GGTAACGGCGACGATCGCGCGAACGGCGCCAATGGCGAAGACGACAGCTACGGCGACAGCGCGGGTGACGATTACGGCAG
CGACTGGAGCCTGGACGATTTCGCTCGCCGGCCTCAGGGCGATGATGACGAGAAGACGCCGATGCAGCTTCGCGAAGCGG
AACCCACGCTGCGCGAATACCTGATGGAACAGCTCACGCCGCTGAAGATCTCCACGCGTGACAAGGGCCTGGCCATCTTC
CTGATCGAGTCGCTTGACGACGACGGCTACCTGGGCGCAACGCTCGACGAGATCTGTTCGGAACTGCCCAAGGAACTCGA
ATTCGAAGTCGATGAAGTGCAGGCGATACTGACCCTGCTGCAGAGCTTCGACCCGCCTGGCGTGGGTGCGCGCAACGCGG
CGGAATGCCTCACCCTGCAACTGCGCCGTCTGCAACATCCGCAGCGGGACCTTGCATTATCGATCGTCAACCACCACCTG
GAGTTATTGGCCGTGCGTGACTACACGCGGCTCAAGAAAGCCCTGCAGGTTGACGAAATTGCACTTAAGGCCGCGCACGA
TCTGATCCGCTCGCTCGCACCGTATCCCGGACATGCCTACAGCCGTCCGGAAGCGGACTTCGTGGTACCGGATGTGTTCG
TGCGCAAGAGTGGCAGTAGCTGGATCGCACAGCTCAATCCCGACGTGATGCCGCGTCTGCGCATCAACGACATGTACGCG
CAGATTTTGCGCGGTGCAAAAGGGGAAGCGGGCGCGGCCGGGCTGCAGCAGAAGCTCCAGGAGGCGCGGTGGCTCATCAA
GAACATTCAGCAGCGTTTCGACACGATCCTGCGTGTTGCGCAGGCAATTGTGGAGCGTCAAAGGAACTTTTTCTCGCACG
GGGAAATCGCGATGCGCCCCTTGGTTTTGCGGGAGATTGCCGATACACTGGGTCTACACGAGTCGACCATTTCCCGGGTG
ACTACGAATAAATACATGGCCACGCCGATGGGTACTTTCGAGTTGAAGTACTTCTTTGGTAGCCACGTCTCCACGGAGAC
TGGCGGCGCGGCCTCATCCACGGCGATCCGCGCGCTGATCAAGCAACTGATAGGAGCCGAAGAACCCAGGAATCCCCTTT
CCGACAGCCGCATCGCCGAGTTGCTGGGCGAACAGGGCTTTGTGGTCGCACGCCGCACGGTGGCCAAGTACCGCGAAGCG
CTGAAGATCCCCGCAGTCAATCTCCGCAAGACTTTGTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(129-319)

Sigma-54, DNA binding domain (Sigma54_DBD)

(335-490)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q1LRN7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

78

100

0.793

Multiple sequence alignment