Detailed information NTG0025778

Bioinformatically predicted

Overview

• Name: pilA
• Type: Machinery gene
• Locus tag: RMET_RS01430
• Genome accession: NC_007973
• Coordinates: 296919..298181 (-)
• Length: 420 a.a.
• NCBI ID: WP_029306758.1
• Uniprot ID: -
• Organism: Cupriavidus metallidurans CH34
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 291919..303181

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
RMET_RS01405 (Rmet_0276)	ntrB	292036..292905 (-)	870	WP_049799764.1	nitrate ABC transporter permease	-
RMET_RS01410 (Rmet_0277)	-	293016..294320 (-)	1305	WP_011515166.1	CmpA/NrtA family ABC transporter substrate-binding protein	-
RMET_RS01415 (Rmet_0278)	-	294684..295319 (-)	636	WP_024569516.1	ANTAR domain-containing response regulator	-
RMET_RS01420 (Rmet_0279)	-	295494..296195 (+)	702	WP_029306759.1	fumarylacetoacetate hydrolase family protein	-
RMET_RS01425 (Rmet_0280)	maiA	296200..296847 (+)	648	WP_011515169.1	maleylacetoacetate isomerase	-
RMET_RS01430 (Rmet_0281)	pilA	296919..298181 (-)	1263	WP_029306758.1	signal recognition particle-docking protein FtsY	Machinery gene
RMET_RS01435 (Rmet_0282)	-	298242..299756 (+)	1515	WP_011515172.1	pitrilysin family protein	-
RMET_RS01440 (Rmet_0283)	-	299765..301129 (+)	1365	WP_011515173.1	pitrilysin family protein	-
RMET_RS01445 (Rmet_0284)	rsmD	301238..301870 (+)	633	WP_011515174.1	16S rRNA (guanine(966)-N(2))-methyltransferase RsmD	-
RMET_RS01450 (Rmet_0285)	coaD	302082..302567 (+)	486	WP_011515175.1	pantetheine-phosphate adenylyltransferase	-
RMET_RS01455 (Rmet_0286)	-	302634..302894 (+)	261	WP_008650933.1	YfhL family 4Fe-4S dicluster ferredoxin	-

Sequence

Protein

• Length: 420 a.a.
• Molecular weight: 44097.19 Da
• Isoelectric Point: 6.9898

>NTDB_id=25778 RMET_RS01430 WP_029306758.1 296919..298181(-) (pilA) [Cupriavidus metallidurans CH34]
MFSFWKKRKAEPAPESVEPPAAAPTPAPAPAPAPLPAPAPAAAPAVTPVAPPVAAPVATPVSAPASTPAPVPTPAPSPVH
VAPPVAPPVVPPVASAPPPASYTPPVIEELELTPPPAQTAEAKRGWMSRLRAGLSKTSRNIGVLFVGVKVDEALFEELET
ALLMADAGVEATEYLLGELRRRIKNDRIETAEGVKAALKDLLTQLLKPLEKTMELGREQPLVMMIAGVNGAGKTTSIGKL
CKHFQSYHQSVLLAAGDTFRAAAREQLTIWGERNNVTVVAQESGDPAAVIFDAVNAARARKIDIVMADTAGRLPTQLHLM
EELKKVKRVIGKSMPTAPHEVLLVIDANTGQNALAQTRAFDDALGLTGLIVTKLDGTAKGGILAAIARQRPVPVYFIGVG
EKVEDLQPFKADEFAEALLG

Nucleotide

• Length: 1263 bp

>NTDB_id=25778 RMET_RS01430 WP_029306758.1 296919..298181(-) (pilA) [Cupriavidus metallidurans CH34]
ATGTTTAGTTTCTGGAAGAAGCGCAAGGCCGAACCGGCTCCCGAATCGGTCGAGCCGCCCGCGGCCGCCCCCACACCGGC
GCCTGCTCCCGCGCCGGCCCCGCTTCCCGCCCCCGCCCCGGCTGCGGCACCTGCGGTAACCCCTGTCGCCCCCCCTGTAG
CGGCACCCGTTGCAACCCCGGTTTCGGCCCCGGCTTCAACACCTGCGCCAGTTCCAACGCCCGCCCCGTCGCCGGTCCAT
GTGGCGCCACCCGTGGCGCCTCCGGTCGTGCCGCCCGTGGCGTCGGCTCCGCCTCCGGCCAGCTATACGCCGCCGGTCAT
CGAGGAGCTGGAGCTGACCCCGCCGCCCGCGCAAACCGCCGAAGCCAAGCGCGGCTGGATGTCGCGGCTGCGTGCCGGCC
TGTCGAAGACCAGCCGCAACATCGGCGTGCTGTTCGTCGGCGTCAAGGTCGACGAGGCGCTGTTCGAGGAACTGGAAACA
GCGCTGCTGATGGCCGATGCCGGAGTGGAGGCCACCGAGTACCTGCTGGGCGAACTGCGCCGCCGCATCAAGAATGATCG
TATCGAAACGGCCGAAGGCGTGAAGGCCGCGCTCAAGGATCTGCTGACGCAGCTGCTCAAGCCGCTCGAGAAGACGATGG
AACTGGGCCGCGAGCAGCCGCTGGTGATGATGATTGCCGGCGTCAACGGCGCGGGCAAGACCACCAGCATCGGTAAGCTC
TGCAAGCATTTCCAGTCCTATCACCAGTCCGTGCTGCTGGCCGCCGGCGACACATTCCGCGCCGCCGCGCGCGAACAACT
CACGATCTGGGGCGAGCGCAACAACGTGACCGTGGTGGCCCAGGAAAGTGGCGACCCAGCCGCCGTGATCTTCGATGCCG
TCAACGCGGCGCGCGCGCGCAAGATCGACATCGTGATGGCAGATACCGCTGGCCGCCTGCCCACGCAGCTTCACCTGATG
GAGGAACTCAAGAAGGTCAAGCGCGTGATCGGCAAGTCGATGCCGACCGCACCGCATGAAGTGCTGCTTGTGATCGACGC
CAATACTGGTCAGAACGCACTGGCCCAGACGCGCGCGTTCGATGACGCGCTCGGCCTGACCGGCCTGATCGTGACCAAGC
TCGACGGCACGGCCAAGGGCGGCATCCTGGCCGCGATCGCGCGCCAGCGTCCGGTACCGGTGTACTTCATCGGCGTGGGC
GAGAAGGTCGAGGACCTGCAGCCGTTCAAGGCGGACGAGTTCGCCGAGGCGCTGCTGGGCTGA

Domains

Predicted by InterproScan.

• SRP54-type protein, GTPase domain (SRP54) (221-420)

• SRP54-type protein, helical bundle domain (SRP54_N) (148-200)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilA	Neisseria gonorrhoeae MS11	49.533	100	0.505