Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   CWR53_RS21185 Genome accession   NZ_CP025035
Coordinates   4719781..4721043 (-) Length   420 a.a.
NCBI ID   WP_100852424.1    Uniprot ID   A0AAD0B3Y3
Organism   Pseudomonas sp. SGAir0191     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4714781..4726043
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CWR53_RS21170 (CWR53_21170) - 4716453..4718045 (-) 1593 WP_100852423.1 SPOR domain-containing protein -
  CWR53_RS21175 (CWR53_21175) aroB 4718061..4719158 (-) 1098 WP_058602920.1 3-dehydroquinate synthase -
  CWR53_RS21180 (CWR53_21180) aroK 4719258..4719776 (-) 519 WP_027913510.1 shikimate kinase AroK -
  CWR53_RS21185 (CWR53_21185) pilQ 4719781..4721043 (-) 1263 WP_100852424.1 type IV pilus secretin PilQ Machinery gene
  CWR53_RS21190 (CWR53_21190) - 4720967..4721983 (-) 1017 WP_100852425.1 pilus assembly protein PilP -
  CWR53_RS21195 (CWR53_21195) - 4721980..4722516 (-) 537 WP_100852426.1 PilN domain-containing protein -
  CWR53_RS21200 (CWR53_21200) pilM 4722513..4723394 (-) 882 WP_100852427.1 type IV pilus biogenesis protein PilM -
  CWR53_RS21205 (CWR53_21205) - 4723569..4726019 (+) 2451 WP_100852428.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 420 a.a.        Molecular weight: 46075.67 Da        Isoelectric Point: 4.9094

>NTDB_id=257550 CWR53_RS21185 WP_100852424.1 4719781..4721043(-) (pilQ) [Pseudomonas sp. SGAir0191]
MSKTWKWLLATLLLPLTAMAAPYQGERLSLNFQDVEVRAVLQVLADHAGINLVASDDVQGSVTLRLEDVPWDQAIDLVLR
SKGLGRRQDGNVLLIAPLSTLSAQSFEDTANSLDVQQQPMRRELMRIHYAKAADLSELLLASLSEDGILAGRGSLGVDER
TNTLVVHQPANRLAEIRQLIAQLDVPVRQVAIEARIVEANVDYQKGLGVRWGGDLYSTVPRPGKDLFVDLGVERGSSLGV
GLLRGDVLLDLELSAMEKSGEGEIISQPKVVTADKETARILKGTEVPYQQSNKNGATTVSFREASLSLEVTPQITPDDSV
VMTVRVTKDEPDYVNALNDVPPIRKNEVNAKVRVENGQTIVIGGVYSTTQNNVVDKVPFLGDLPYVGRLFRRDVLQEKKS
ELLVFLTPRIMSDQTIAVSR

Nucleotide


Download         Length: 1263 bp        

>NTDB_id=257550 CWR53_RS21185 WP_100852424.1 4719781..4721043(-) (pilQ) [Pseudomonas sp. SGAir0191]
ATGAGCAAGACATGGAAATGGCTACTCGCAACCCTGCTGTTGCCGTTGACGGCAATGGCCGCCCCCTACCAGGGTGAACG
GCTGTCACTGAATTTTCAGGATGTCGAGGTACGCGCCGTGCTGCAGGTGCTTGCCGACCATGCCGGTATCAACCTGGTCG
CCAGCGACGACGTCCAAGGGAGCGTCACCTTGCGGCTGGAAGATGTCCCCTGGGATCAAGCGATCGACCTGGTATTGCGC
AGCAAGGGCCTGGGTCGACGCCAGGACGGCAATGTACTGCTGATCGCACCGCTTTCGACCCTGAGCGCGCAGTCGTTCGA
GGACACCGCAAATTCACTCGACGTGCAACAACAGCCCATGCGTCGAGAACTGATGCGTATCCACTATGCCAAGGCCGCAG
ATTTGTCCGAGCTATTGTTGGCGAGCCTGTCTGAGGACGGCATCCTTGCCGGCAGGGGCAGCCTGGGTGTGGATGAGCGT
ACCAACACGCTGGTGGTCCACCAGCCGGCGAATCGACTTGCCGAGATTCGGCAACTGATCGCCCAGCTGGATGTGCCCGT
GCGTCAGGTCGCGATCGAAGCCCGTATCGTGGAGGCCAACGTCGACTACCAGAAAGGCCTGGGGGTGCGATGGGGTGGCG
ACCTGTACAGCACCGTGCCGAGACCGGGCAAGGACCTGTTCGTTGACTTGGGCGTCGAGCGGGGCAGCAGCCTTGGCGTT
GGTCTGTTGCGCGGCGACGTGCTCCTTGATCTTGAGCTCAGCGCCATGGAAAAGAGCGGTGAGGGCGAGATCATCTCGCA
GCCCAAGGTAGTGACCGCTGACAAGGAGACGGCGCGGATACTCAAAGGTACCGAGGTGCCTTACCAGCAGTCCAACAAGA
ACGGCGCTACAACCGTATCGTTTCGCGAGGCTTCGCTTTCACTGGAAGTAACGCCGCAGATCACGCCCGATGACAGTGTC
GTCATGACCGTGAGAGTGACCAAGGACGAACCTGACTACGTCAATGCCCTGAATGACGTCCCGCCCATTCGCAAGAACGA
GGTCAATGCCAAGGTCAGGGTCGAGAACGGGCAGACCATCGTGATCGGTGGCGTTTACTCGACCACGCAAAACAATGTGG
TGGACAAGGTGCCATTTCTTGGCGATCTGCCGTATGTTGGACGGCTGTTTCGACGCGATGTATTACAAGAGAAAAAATCC
GAGCTGCTGGTCTTCCTGACTCCGCGTATCATGAGTGACCAGACGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(125-189)

Secretin and TonB N terminus short domain (STN)

(51-97)

Bacterial type II and III secretion system protein (Secretin)

(255-411)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

54.773

100

0.574
  comQ Acinetobacter baylyi ADP1

42.572

100

0.457
  pilQ Acinetobacter baumannii D1279779

41.83

100

0.457
  pilQ Acinetobacter baumannii strain A118

41.83

100

0.457
  pilQ Legionella pneumophila strain Lp02

41.866

99.524

0.417
  pilQ Legionella pneumophila strain ERS1305867

41.866

99.524

0.417
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

41.148

99.524

0.41
  pilQ Vibrio cholerae strain A1552

41.148

99.524

0.41
  pilQ Vibrio campbellii strain DS40M4

39.906

100

0.405

Multiple sequence alignment