Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   CWR52_RS15000 Genome accession   NZ_CP025034
Coordinates   3122215..3123648 (-) Length   477 a.a.
NCBI ID   WP_104950015.1    Uniprot ID   -
Organism   Enterobacter sp. SGAir0187     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3117215..3128648
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CWR52_RS14970 (CWR52_15095) elbB 3118745..3119398 (+) 654 WP_104950927.1 isoprenoid biosynthesis glyoxalase ElbB -
  CWR52_RS14975 (CWR52_15100) mtgA 3119395..3120120 (+) 726 WP_104950014.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  CWR52_RS14980 (CWR52_15105) npr 3120162..3120434 (-) 273 WP_003861872.1 PTS phosphocarrier protein NPr -
  CWR52_RS14985 (CWR52_15110) rapZ 3120431..3121285 (-) 855 WP_014885397.1 RNase adapter RapZ -
  CWR52_RS14990 (CWR52_15115) ptsN 3121331..3121822 (-) 492 WP_008501443.1 PTS IIA-like nitrogen regulatory protein PtsN -
  CWR52_RS14995 (CWR52_15120) hpf 3121905..3122192 (-) 288 WP_013098943.1 ribosome hibernation promoting factor -
  CWR52_RS15000 (CWR52_15125) rpoN/rpoN1 3122215..3123648 (-) 1434 WP_104950015.1 RNA polymerase factor sigma-54 Machinery gene
  CWR52_RS15005 (CWR52_15130) lptB 3123696..3124421 (-) 726 WP_008501446.1 LPS export ABC transporter ATP-binding protein -
  CWR52_RS15010 (CWR52_15135) lptA 3124428..3124982 (-) 555 WP_020883526.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  CWR52_RS15015 (CWR52_15140) lptC 3124951..3125526 (-) 576 WP_021242266.1 LPS export ABC transporter periplasmic protein LptC -
  CWR52_RS15020 (CWR52_15145) kdsC 3125523..3126089 (-) 567 WP_008501449.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  CWR52_RS15025 (CWR52_15150) kdsD 3126104..3127090 (-) 987 WP_008501450.1 arabinose-5-phosphate isomerase KdsD -
  CWR52_RS15030 (CWR52_15155) - 3127104..3128081 (-) 978 WP_045334591.1 calcium/sodium antiporter -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 54125.88 Da        Isoelectric Point: 4.3332

>NTDB_id=257522 CWR52_RS15000 WP_104950015.1 3122215..3123648(-) (rpoN/rpoN1) [Enterobacter sp. SGAir0187]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALDSNPLLEQTDLHDEVDTQQSQDTEALDTADALEQKEMPD
ELPLDASWDEIYTAGTPSGTRADYQDDELPVYQGETTQSLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVTLDD
ILESMGDDEIELEEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFSKETPWLDEARVIISDHLDLLANHDFRSLMRVTR
LKEEVLKEAVNLIQSLDPRPGQSIQTSEPEYVIPDVLVRKHNGRWVVELNSDSIPRLQINQQYASMCTSVRNDSDNQYIR
SNLQEARWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTTMLSDQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=257522 CWR52_RS15000 WP_104950015.1 3122215..3123648(-) (rpoN/rpoN1) [Enterobacter sp. SGAir0187]
ATGAAGCAAGGTTTGCAATTAAGGCTCAGCCAACAACTGGCGATGACGCCGCAGCTACAGCAGGCAATTCGCCTGCTGCA
ACTGTCCACGTTAGAACTTCAGCAGGAGCTCCAGCAGGCGCTGGACAGCAACCCCCTGCTGGAACAAACCGATCTTCATG
ACGAGGTAGATACCCAGCAATCGCAGGATACAGAAGCGCTCGACACCGCGGATGCACTCGAACAAAAAGAGATGCCGGAC
GAGCTTCCGCTGGATGCCAGCTGGGATGAAATCTACACCGCCGGAACCCCTTCCGGTACGCGTGCAGACTACCAGGATGA
TGAGCTACCGGTCTACCAGGGCGAAACCACCCAGTCACTGCAGGATTACCTGATGTGGCAGGTGGAACTTACTCCCTTCT
CCGATACCGACCGCGCGATTGCCACCTCAATTGTCGATGCCGTTGACGACACTGGCTATTTGACCGTCACGCTGGACGAT
ATTCTGGAAAGCATGGGCGATGACGAGATAGAGCTTGAGGAAGTTGAAGCCGTTCTGAAGCGCATCCAGCGTTTCGATCC
GGTAGGCGTGGCCGCAAAAGATCTGCGCGACTGCCTGCTGATCCAGCTTTCTCAGTTCAGCAAAGAGACGCCGTGGCTCG
ACGAGGCGCGCGTAATCATCAGCGATCATCTGGATCTGCTGGCGAACCACGATTTCCGCTCCCTGATGCGCGTCACGCGC
CTGAAGGAAGAGGTGCTGAAAGAAGCGGTGAATCTGATCCAGTCGCTCGATCCGCGCCCGGGACAGTCGATCCAGACCAG
CGAGCCCGAGTACGTCATCCCGGATGTGCTGGTCAGAAAACATAATGGCCGCTGGGTCGTTGAACTGAACTCTGACAGCA
TCCCTCGCCTGCAAATCAACCAGCAGTATGCCTCCATGTGCACCAGCGTGCGTAACGACTCCGACAATCAATATATTCGT
AGCAATTTGCAGGAAGCGCGATGGCTGATCAAAAGTCTGGAGAGCCGAAATGATACGCTGCTACGCGTCAGCCGCTGTAT
TGTCGAACAGCAACAGGCGTTCTTTGAGCAGGGCGAAGAGTATATGAAACCGATGGTGCTGGCGGATATCGCCCAGGCCG
TCGAGATGCATGAATCAACCATTTCCCGTGTCACCACGCAGAAGTATCTGCACAGTCCACGCGGTATTTTTGAGCTTAAG
TATTTCTTCTCCAGCCATGTGAATACCGAGGGCGGCGGCGAAGCGTCGTCCACGGCCATTCGTGCCCTGGTGAAGAAGTT
GATCGCCGCGGAGAACCCCGCGAAGCCGCTAAGCGACAGTAAGTTAACCACCATGCTGTCCGATCAGGGTATTATGGTGG
CACGTCGTACTGTTGCGAAGTATCGAGAGTCTTTATCCATTCCGCCGTCCAACCAGCGTAAACAGCTGGTCTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.347

100

0.48

Multiple sequence alignment