Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   BPRO_RS22435 Genome accession   NC_007948
Coordinates   4739321..4740868 (-) Length   515 a.a.
NCBI ID   WP_011485359.1    Uniprot ID   -
Organism   Polaromonas sp. JS666     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4734321..4745868
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BPRO_RS22410 (Bpro_4479) - 4734432..4734902 (+) 471 WP_011485353.1 OsmC family protein -
  BPRO_RS30190 (Bpro_4481) - 4735836..4736165 (+) 330 WP_198140962.1 hypothetical protein -
  BPRO_RS22420 (Bpro_4482) - 4736267..4737091 (-) 825 WP_011485356.1 UPF0149 family protein -
  BPRO_RS22425 (Bpro_4483) - 4737316..4737726 (-) 411 WP_011485357.1 putative toxin-antitoxin system toxin component, PIN family -
  BPRO_RS22430 (Bpro_4484) - 4737776..4739182 (-) 1407 WP_011485358.1 THUMP domain-containing class I SAM-dependent RNA methyltransferase -
  BPRO_RS22435 (Bpro_4485) rpoN/rpoN1 4739321..4740868 (-) 1548 WP_011485359.1 RNA polymerase factor sigma-54 Machinery gene
  BPRO_RS22440 (Bpro_4486) lptB 4741011..4741808 (-) 798 WP_049764160.1 LPS export ABC transporter ATP-binding protein -
  BPRO_RS22445 (Bpro_4487) lptA 4741831..4742448 (-) 618 WP_011485361.1 lipopolysaccharide transport periplasmic protein LptA -
  BPRO_RS22450 (Bpro_4488) - 4742611..4743273 (-) 663 WP_011485362.1 thiol:disulfide interchange protein DsbA/DsbL -
  BPRO_RS22455 (Bpro_4489) - 4743393..4744013 (-) 621 WP_011485363.1 SPOR domain-containing protein -
  BPRO_RS22460 (Bpro_4490) argS 4744083..4745768 (-) 1686 WP_011485364.1 arginine--tRNA ligase -

Sequence

Protein


Download         Length: 515 a.a.        Molecular weight: 56985.09 Da        Isoelectric Point: 4.7491

>NTDB_id=25685 BPRO_RS22435 WP_011485359.1 4739321..4740868(-) (rpoN/rpoN1) [Polaromonas sp. JS666]
MKQGLSLRVSQHLALTPQLQQSIRLLQLSTLELSQEVEQMLDENPFLEVSEEAAPREEFGLEQTDTPVSQDSREFEFAAD
SVAVNGDESRAAGQNDADIGPEVSSEAKLEESWEGDGSVESSPDDSEWGGDAPARKSNADDSEAEASDLARSHESLQDHL
HRQSLGLRLSEEDQAALHFLIESLNDDGYLEDSLASLAAGLAGDDLEQAEELEQHFAMALKLLQHMEPAGVGARNLAECL
GLQLLDCKDCDETRAAIAMCKQPMELLAKRDVKRLSYLCGFPDSVIRGAIAVIGRLDPKPGRRFVNVERNLIVPDVIVVK
SGRGFKVSLNSEVMPRLRVHDIYANALKQHKGQNGQALQQRLQEARWFIKNIQQRFDTILRVSSAIVERQKNFFTHGELA
MRPLVLREIADELGLHESTISRVTTAKYMSTPFGTFELKYFFGSALGTETGGNASSTAVRALIKQFVSSESLKKPLSDNQ
ISEMLKEQGIECARRTVAKYREALRIAPANLRKSL

Nucleotide


Download         Length: 1548 bp        

>NTDB_id=25685 BPRO_RS22435 WP_011485359.1 4739321..4740868(-) (rpoN/rpoN1) [Polaromonas sp. JS666]
GTGAAGCAAGGGCTTTCTCTCCGCGTCTCGCAGCATCTCGCGCTCACGCCGCAGCTGCAGCAGTCGATTCGCCTGCTGCA
GCTGTCCACGCTTGAGCTGAGCCAGGAAGTCGAGCAGATGCTCGATGAAAATCCGTTTCTGGAGGTGTCTGAAGAAGCCG
CCCCGCGCGAGGAATTTGGCCTGGAACAGACCGACACGCCGGTCAGCCAGGACAGCCGGGAGTTCGAATTTGCTGCAGAT
TCAGTAGCTGTCAATGGCGATGAATCGAGGGCTGCAGGCCAAAATGATGCCGACATTGGCCCCGAGGTCAGTTCCGAGGC
CAAGCTGGAGGAAAGCTGGGAGGGCGACGGCTCGGTGGAGTCCTCGCCTGACGACAGCGAATGGGGCGGCGACGCGCCGG
CCCGCAAGAGCAATGCGGACGACAGCGAGGCCGAGGCTTCCGACCTGGCCCGCAGCCATGAGTCGCTGCAGGACCATTTG
CATCGCCAGTCGCTCGGCCTGCGCCTGTCGGAGGAAGACCAGGCGGCACTGCATTTCCTGATCGAGTCGCTCAATGACGA
CGGCTACCTTGAAGACAGTCTCGCGTCACTGGCCGCTGGCCTGGCGGGCGACGACCTTGAGCAGGCCGAAGAGCTGGAAC
AGCACTTTGCCATGGCCCTGAAGCTGCTGCAGCACATGGAGCCGGCCGGTGTCGGCGCCCGCAACCTGGCCGAGTGCCTC
GGCCTGCAACTGCTCGACTGCAAGGACTGCGACGAGACCCGCGCAGCGATCGCCATGTGCAAGCAGCCCATGGAGCTGCT
GGCCAAGCGGGACGTCAAGCGCCTGTCCTACCTGTGCGGGTTTCCGGACAGCGTGATCAGGGGCGCCATCGCCGTGATCG
GCCGGCTGGATCCCAAGCCCGGGCGGCGGTTTGTCAACGTCGAGCGCAACCTCATCGTGCCCGACGTGATCGTCGTCAAG
TCGGGCCGCGGCTTCAAGGTCTCCCTCAACAGCGAGGTCATGCCCCGCCTGCGCGTGCACGACATCTATGCCAATGCGCT
GAAGCAGCACAAGGGCCAGAACGGGCAGGCCTTGCAGCAGCGCTTGCAGGAAGCGCGCTGGTTCATCAAGAACATCCAGC
AGCGCTTTGACACCATCCTGCGCGTGAGCAGCGCGATTGTGGAGCGGCAAAAGAATTTCTTCACCCACGGCGAGCTGGCC
ATGCGTCCGCTGGTGCTGCGCGAAATTGCCGACGAACTGGGCCTGCACGAGTCCACCATCTCGCGCGTCACCACGGCCAA
GTACATGAGCACGCCGTTCGGCACCTTCGAGCTCAAGTACTTTTTCGGCTCCGCGCTGGGCACCGAGACTGGTGGTAACG
CATCCAGCACCGCGGTGCGCGCACTGATCAAGCAGTTTGTCTCGTCCGAGAGCCTGAAAAAACCGCTCAGCGACAACCAG
ATTTCCGAAATGCTCAAGGAGCAGGGCATTGAATGCGCGCGCCGCACCGTGGCCAAGTACCGCGAAGCTTTGCGCATCGC
GCCTGCCAACCTGCGCAAATCGCTTTGA

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(357-513)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-49)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(148-343)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

52.326

100

0.524

Multiple sequence alignment