Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   MFLA_RS06810 Genome accession   NC_007947
Coordinates   1413883..1415244 (+) Length   453 a.a.
NCBI ID   WP_011479554.1    Uniprot ID   Q1H1N7
Organism   Methylobacillus flagellatus KT     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1408883..1420244
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MFLA_RS06780 (Mfla_1326) rpsF 1409899..1410294 (+) 396 WP_011479548.1 30S ribosomal protein S6 -
  MFLA_RS06785 (Mfla_1327) priB 1410304..1410597 (+) 294 WP_048811608.1 primosomal replication protein N -
  MFLA_RS06790 (Mfla_1328) rpsR 1410610..1410837 (+) 228 WP_011479550.1 30S ribosomal protein S18 -
  MFLA_RS06795 (Mfla_1329) rplI 1410849..1411295 (+) 447 WP_011479551.1 50S ribosomal protein L9 -
  MFLA_RS06800 (Mfla_1330) dnaB 1411419..1412807 (+) 1389 WP_011479552.1 replicative DNA helicase -
  MFLA_RS06805 (Mfla_1331) alr 1412810..1413883 (+) 1074 WP_011479553.1 alanine racemase -
  MFLA_RS06810 (Mfla_1332) radA 1413883..1415244 (+) 1362 WP_011479554.1 DNA repair protein RadA Machinery gene
  MFLA_RS14995 - 1415939..1416061 (-) 123 WP_267864486.1 hypothetical protein -
  MFLA_RS06820 (Mfla_1333) - 1416234..1416728 (-) 495 WP_011479555.1 type II secretion system protein -
  MFLA_RS06825 (Mfla_1334) - 1416715..1417116 (-) 402 WP_011479556.1 type II secretion system protein -
  MFLA_RS06830 (Mfla_1335) - 1417119..1417604 (-) 486 WP_011479557.1 type II secretion system protein -
  MFLA_RS06835 (Mfla_1336) - 1417601..1419508 (-) 1908 WP_195742153.1 secretin N-terminal domain-containing protein -
  MFLA_RS06840 (Mfla_1337) - 1419559..1420092 (-) 534 WP_011479559.1 hypothetical protein -

Sequence

Protein


Download         Length: 453 a.a.        Molecular weight: 48326.82 Da        Isoelectric Point: 7.6655

>NTDB_id=25654 MFLA_RS06810 WP_011479554.1 1413883..1415244(+) (radA) [Methylobacillus flagellatus KT]
MAKAKTAYSCTECGGQTPKWQGQCPSCMAWNTLVESVVEAAPSQNRYAALAQSSGLQKLADVEAADVPRHPTGIHEFDRV
LGGGLVPGGVVLIGGDPGIGKSTLLLQTLCHIGGMRKAVYVSGEESAQQIAMRAKRLGLDAGPVELLAEISLEKILAILQ
QHKPEVAVIDSIQTLYSEALQSAPGSVAQVRECSAQLTRFAKQTGVTMILVGHVTKEGSLAGPRVLEHIVDTVLYFEGGQ
NSSFRLVRAFKNRFGAVNELGVFAMTEKGLREVSNPSALFLSHHAEQVAGSCITVTQEGSRPLLVEVQALVDEAHAPNPK
RLCVGLEQNRLAMLLAVLHRHAGVACFDQDVFINAVGGVRISEPAVDLAVLLAIVSSLKNKPLNNKLIVFGEVGLAGEVR
PVQRGQERLREAAKLGFTHAIVPKANLPKQVIDGLDVKGVERLDQALQLLRNG

Nucleotide


Download         Length: 1362 bp        

>NTDB_id=25654 MFLA_RS06810 WP_011479554.1 1413883..1415244(+) (radA) [Methylobacillus flagellatus KT]
ATGGCGAAGGCAAAAACGGCGTATAGCTGTACCGAGTGCGGCGGACAAACCCCGAAGTGGCAAGGGCAATGTCCCTCGTG
CATGGCATGGAATACATTGGTCGAAAGCGTGGTGGAAGCTGCCCCCAGCCAGAACCGTTATGCCGCGCTTGCGCAATCCA
GCGGTTTGCAGAAGTTGGCCGATGTCGAGGCTGCGGATGTGCCGCGCCACCCAACGGGCATCCACGAATTCGATCGGGTT
CTGGGTGGGGGGCTGGTGCCGGGCGGCGTGGTGTTGATCGGGGGAGATCCCGGCATCGGCAAGTCCACCTTGCTATTGCA
GACCTTGTGTCATATCGGCGGTATGCGCAAGGCCGTCTATGTCTCAGGTGAAGAGTCCGCACAGCAGATCGCCATGCGTG
CCAAACGATTGGGCCTTGACGCCGGTCCGGTCGAGCTGCTGGCGGAAATCAGCCTCGAAAAGATCTTGGCTATCCTGCAG
CAGCACAAGCCCGAAGTGGCTGTTATCGACTCGATACAGACCTTGTACTCCGAGGCCTTGCAATCTGCGCCTGGTTCCGT
GGCGCAAGTCCGGGAATGCTCCGCCCAGCTGACGCGTTTCGCCAAGCAGACCGGTGTGACCATGATTTTGGTCGGGCACG
TGACCAAGGAGGGATCTCTCGCCGGACCACGCGTCCTGGAGCATATAGTCGATACCGTACTGTATTTCGAAGGGGGCCAG
AACTCCAGCTTCCGGCTGGTGCGCGCCTTCAAAAACCGTTTTGGCGCCGTGAACGAACTGGGCGTGTTCGCCATGACCGA
GAAGGGGCTGCGCGAGGTCAGCAATCCTTCAGCGTTATTCCTGTCGCATCATGCAGAACAGGTTGCAGGTTCCTGCATCA
CCGTAACGCAGGAAGGGAGCAGGCCGCTCCTGGTGGAAGTGCAGGCACTGGTGGACGAGGCGCATGCGCCCAATCCCAAG
CGCCTGTGCGTCGGCCTGGAACAGAACCGGCTTGCCATGCTGCTGGCCGTCCTGCACCGCCATGCCGGCGTAGCCTGCTT
TGACCAGGACGTATTCATCAATGCCGTTGGGGGCGTCAGGATCAGCGAGCCTGCTGTGGATCTCGCCGTGCTGCTTGCAA
TTGTTTCCTCACTAAAAAACAAACCCCTGAACAATAAATTGATCGTATTCGGCGAGGTTGGCCTGGCAGGCGAGGTCCGC
CCGGTGCAGCGCGGCCAGGAGCGCTTGCGGGAGGCCGCCAAGCTCGGCTTCACCCACGCCATCGTTCCCAAGGCCAACTT
GCCCAAGCAAGTGATCGATGGCTTGGACGTCAAAGGAGTGGAGCGCCTCGACCAGGCTTTGCAGCTGTTGCGTAACGGGT
GA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(79-218)

Subunit ChlI of Mg-chelatase (ChlI)

(343-426)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q1H1N7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

49.345

100

0.499
  radA Streptococcus pneumoniae Rx1

47.903

100

0.479
  radA Streptococcus pneumoniae D39

47.903

100

0.479
  radA Streptococcus pneumoniae R6

47.903

100

0.479
  radA Streptococcus pneumoniae TIGR4

47.903

100

0.479
  radA Streptococcus mitis NCTC 12261

47.682

100

0.477
  radA Streptococcus mitis SK321

49.415

94.26

0.466

Multiple sequence alignment